INVESTIGATION OF CYTOSTATIC ACTIVITY OF THE METHENAMINE SILVER IN VITRO AND IN VIVO
Keywords:methenamine silver nitrate, HUT 102 cells, lymphocytes, leucosis, cytostatic activity, bovine leukemia, silver hexamethylenetetramine
Background: Developing of novel pharmaceutic agents is an actual topic for chemotherapy. Heavy metals possess a significant cytotoxic properties and some of them are widly introduced in medical practice, e.g. cisplatin.
Aim and Objective of this study was to investigate the cytostatic effects of prospective silver-based preparation on viral bovine leukemia model in vivo and lymphoblast cells in vitro.
Material and Methods: Silver salt of methenamine was suggested as prospective substance and tested was tested on black-motley cattle with bovine leukemia to reveal influence on hematological parameters. Preparation was applied in form of 0.5% solution in different doses and was administered as single intravenous injection. Blood parameters were determined by triple hematological monitoring. Investigation of cytostatic activity was conducted on the leukemic cells of HUT-102 line in Vitro in concentration range of 0.03-3.0 mg/ml.
Results: The results showed normalization of blood parameters in dose-dependent manner after intravenous injection of methenamine silver salt. It was revealed a pronounced inhibitory effect of silver-based drug on the leukemic cells both in vitro and in vivo. Control blood test one month after experimental therapy confirmed the stability of the achieved results.
Conclusion: The tested drug showed a moderate cytostatic effect. Methenamine silver nitrate can be considered as prospective pharmacological agent. Bovine leukemia was shown as a convenient model for the development of new treatment of blood diseases. Continuation of the work is of interest for both veterinary and medical pharmacotherapy.
Keywords: methenamine silver nitrate, HUT 102 cells; lymphocytes, leucosis, cytostatic activity, bovine leukemia, silver hexamethylenetetramine.
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