ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES OF OCIMUM GRATISSIMUM L.
DOI:
https://doi.org/10.22159/ijpps.2017v9i12.22678Keywords:
Ocimum gratissimum, essential oil, antibacterial, antifungalAbstract
The development of antibiotic resistance in microorganisms is a global challenge for the clinicians, pharmacist and research scientists leading to development of new medicinal formulations that are effective and easily consumable. The plant yielding essential oil with chief constituent as eugenol has been identified as an important compound with strong inhibition of bacteria, and storage fungi. Ocimum gratissimum is an aromatic shrub occurring in warm tropical regions has been used in traditional medicine in India to cure various ailments in general and as an antimicrobial agent in particular. The literature surveyed reveals that the plant oil exhibit strong potentiality against gram negative and gram positive bacteria along with diverse human and plant fungi. As a natural product the plant oil is safer as food preservatives and stored grain protectant and has wide application in cosmetics and perfumery industries. This comprehensive review is attempted to provide the valuable information on antimicrobial activities of the plant essential oil that will be used to explore and develop a standard therapeutics system for the management of clinical and multiple drug resistant microorganisms.
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Brown ED, Wright GD. Antibacterial drug discovery in the resistance era. Nature 2016;529:336–43.
Adeola SA, Folorunso OS, Okedeyi OO, Ogungbe BF, Babatimehin OB, Thanni OZ. Antioxidant and antimicrobial activities of the volatile oil of Ocimum gratissimum and its inhibition on partially purified and characterized extracellular protease of Salmonella enteritidis. Am J Drug Dis Dev 2014;4:180-93.
Mohammadpour N, Emami SA, Asili J. Identification of volatile oil components of Nepeta binaludensis Jamzad by GC-MS and 13C-NMR methods and evaluation of its antimicrobial activity. J Essent Oil-Bear Plants 2013;16:102-7.
Shukla S, Hegde S, Kumar A, Chaudhary G, Tewari SK, Upreti DK, et al. Chemical composition and antibacterial activity of essential oil from leaves of Justicia adhatoda against methicillian resistant and sensitive strain along with their clinical isolates. J Essent Oil-Bear Plants 2017;20:116-22.
Hossain MA, Kabir MJ, Salehuddin SM, Rahman SMM, Das AK, Singha SK, et al. Antibacterial properties of essential oils and methanol extracts of sweet basil Ocimum basilicum occurring in Bangladesh. Pharm Biol 2010;48:504-11.
Kumar S, Pandey S. An ethnobotanical study of local plants and their medicinal importance in Tons river area, Dehradun, Uttarakhand. Indian J Trop Biodiversity 2015;23:227-31.
Maryam I, Huzaifa U, Hindatu H, Zubaida S. Nanoencapsulation of essential oils with enhanced antimicrobial activity: a new way of combating antimicrobial Resistance. J Pharma Phytochem 2015;4:165-70.
Vadhana P, Singh BR, Bharadwaj M, Singh SV. The emergence of herbal antimicrobial drug resistance in clinical bacterial isolates. Pharm Anal Acta 2015;6:434.
Tak Jun-Hyung, Isman MB. Penetration-enhancement underlies synergy of plant essential oil terpenoids as insecticides in the cabbage looper, Trichoplusiani. Sci Rep 2017;7:424-32.
Swamy MK, Akhtar MS, Sinniah UR. Antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review. J Evidence-Based Complementary Altern Med 2016. http://dx.doi.org/10.1155/2016/3012462
George M, Britto SJ. Phytochemical, antioxidant and antibacterial studies on the leaf extracts of curcuma amada Roxb. Int J Curr Pharm Res 2016;8:32-8.
Tyagi R, Sharma G, Jasuja ND, Menghani E. Indian medicinal plants as an effective antimicrobial agent. J Crit Rev 2016;3:69-71.
Hu Qing-Ping, Cao Xin-Ming, Hao Dong-Lin, Zhang, Liang-Liang. Chemical composition, antioxidant, DNA damage protective, cytotoxic and antibacterial activities of Cyperus rotundus rhizomes essential oil against foodborne pathogens. Sci Rep 2017;7:45231.
Osuntokun OT, Ogunleye AJ. Prospects of essential oils in drug discovery. Adv Cytol Pathol 2017;2:00010.
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
Kalemba D, Kunicka A. Antibacterial and antifungal properties of essential oils. Curr Med Chem 2003;10:813-29.
Sharifi-Rad J, Sureda A, Tenore GC, Daglia M, Sharifi-Rad M, Valussi M, et al. Biological activities of essential oils: from plant chemoecology to traditional healing systems. Molecules 2017;22:70.
Yap PSX, Yiap BC, Ping HC, Lim SHE. Essential oils, a new horizon in combating bacterial antibiotic resistance. Open Microbiol J 2014;8:6-14.
Silva VA, da Sousa JP, Pessôa HDF, de Freitas AFR, Coutinho HDM, Alves LBN, et al. Ocimum basilicum: antibacterial activity and association study with antibiotics against bacteria of clinical importance. Pharm Biol 2016;54:863-7.
Laurence BH. Labiatae oils-mother nature's chemical factory. XIth Intial Congr. Essent. Oils, Fragrances-and Flavours. New Delhi; 1989.
Svendsen AB, Scheffer JJC. Isolation and analysis of essential oils. In: Verghese J. Essential–Oils. Synthite, Kolenchery, India; 1986.
Alexander P. Phytochemical screening and mineral composition of the leaves of Ocimum gratissimum (Scent leaf). Int J Appl Sci Biotechnol 2016;4:161-5.
Macdonald IO, Oludare AS, Olabiyi A. Phytotoxic and anti-microbial activities of flavonoids in Ocimum gratissimum. Life Sci J 2010;7:45-8.
Koba K, Sanda K, Guyon C, Raynaud C, Millet J, Chaumont JP, et al. chemical composition and in vitro cytotoxic activity of essential oils from two tropical Lamiaceae: Aeollanthus pubescens Benth and Ocimum gratissimum L. J Essent Oil Bear Plants 2007;10:60-9.
Matasyoh LG, Matasyoh JC, Wachira FN, Kinyua MG, Thairu MAW, Mukiama TK. Antimicrobial activity of essential oils of Ocimum Gratissimum L. from different populations of Kenya. Afr J Tradit Complementary Altern Med 2008;5:187-93.
Mith H, Yayi-Ladékan E, Kpoviessi SDS, Bokossa IY, Moudachirou M, Daube G, et al. Chemical composition and antimicrobial activity of essential oils of Ocimum basilicum, Ocimum canum and Ocimum gratissimum in function of harvesting time. J Essent Oil Bear Plants 2016;19:1413-25.
Nakamura CV, Ueda-Nakamura T, Bando E, Melo AFN, Cortez DAG, Dias F, et al. Antibacterial activity of Ocimum gratissimum L. essential oil. Memdo Inst Oswaldo Cruz 1999;94:675-8.
Joshi RK. Chemical composition, in vitro antimicrobial and antioxidant activities of the essential oils of Ocimum gratissimum, O. Sanctum and their major Constituents. Indian J Pharm Sci 2013;75:457-62.
Singh P, Jayaramaiah RH, Agawane SB, Vannuruswamy G, Korwar AM, Anand A, et al. Potential dual role of eugenol in inhibiting advanced glycation end products in diabetes: proteomic and mechanistic insights. Sci Rep 2016;6:18798.
Katara A, Pradhan CK, Singh P, Singh V, Ali M. Volatile constituents and antimicrobial activity of aerial parts of Ocimum gratissimum Linn. J Essent Oil Bear Plants 2013;16:283-8.
Marchese A, Ramona B, Erika C, Ilkay EO, Maria D, Seyed FN, et al. Antimicrobial activity of eugenol and essential oils containing eugenol: a mechanistic viewpoint. Crit Rev Microbiol 2017;43:668-89.
Pradeepa M, Kalidas V, Geetha N. Qualitative and quantitative phytochemical analysis and bactericidal activity of Pelargonium graveolens L’Her. Int J Appl Pharm 2016;8:7-11.
Hemalatha S, Akther T, Khan MS. Extraction of flavonoid from various parts of Couroupita guianensis and its efficacy against pathogenic bacteria. Asian J Pharm Clin Res 2017;10:354-8.
Pandey S, Shukla A, Pandey S, Pandey A. Morphology, chemical composition and therapeutic potential of Somlata (Sarcostemma acidum Wight and Arn.). Pharma Sci Monit 2017;8:54-60.
Mbata T, Saikia A. Antibacterial activity and phytochemical screening of crude ethanolic extract of leaves of Ocimum gratissimum L on listeria monocytogenes. Internet J Microbiol 2007;4:1-6.
Nguefack J, Nguikwie SK, Fotio D, Dongmo B, Amvam Zollo PH, Leth V, et al. Fungicidal potential of essential oils and fractions from Cymbopogon ainsliecitratus, Ocimum gratissimum and Thymus vulgaris to control Alternaria padwickii and Bipolaris oryzae, two seed-borne fungi of rice (Oryza sativa L.). J Essent Oil Res 2007;19:581-7.
Nweze EI, Eze EE. The justification for the use of Ocimum gratissimum L in herbal medicine and its interaction with disc antibiotics. BMC Complementary Altern Med 2009;9:37.
Pandey S, Shukla A, Pandey S, Pandey A. An overview of resurrecting herb ‘Sanjeevani’ (Selaginella bryopteris) and its pharmacological and ethnomedicinal uses. J Pharm Innovation 2017;6:11-4.
Gulabkunverha. Charaka samhita: an English, Hindi and Gujarati translation. Jamnagar (India): Shree Gulabkunerba Ayurvedic Society; 1949.
Ainslie W. Materia Indica. Vol. 1. London: Longman Rees Orme Brown and Green; 1826.
Ahonkhai I, Ba A, Edogun O, Mu U. Antimicrobial activities of the volatile oils of Ocimum bacilicum L. and Ocimum gratissimum L. (Lamiaceae) against some aerobic dental isolates. Pak J Pharm Sci 2009;22:405-9.
Sartoratto A, Machado ALM, Delarmelina C, Figueira GM, Duarte MCT, Rehder VLG. Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Braz J Microbiol 2004;35:275-80.
Olamide SO, Agu GC. The assessment of the antimicrobial activities of Ocimum gratissimum (Wild Basil) and Vernonia Amygdalina (Bitter Leaf) on some enteric pathogen causing dysentery or diarrhea in patients. Int J Eng Sci 2013;2:83-96.
Ilori M, Sheteolu AO, Omonigbehin EA, Adeneye AA. Antidiarrhoeal activities of Ocimum gratissimum (Lamiaceae). J Diarrhoeal Dis Res 1996;14:283-5.
Akinyemi KO, Oladapo O, Okwara CE, Ibe CC, Fasure KA. Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity. BMC Complementary Altern Med 2005;5:6.
Amengialue OO, Edobor O, Egharevba AP. Antibacterial activity of extracts of Ocimum gratissimum on bacteria associated with diarrhea. Bayero J Pure Appl Sci 2013;6:143-5.
Okechukwu OI, Tahir F. Phytochemical and antimicrobial screening of the leaf extract of Ocimum gratissimum L. J Econ Taxon Bot 2008;32:171-7.
Mishra RP, Javed I. In vitro activity of medicinal plants against some bacterial and fungal isolates. Asian J Pharm Clin Res 2015;8:225-30.
Mann A. Phytochemical constituents and antimicrobial and grain protectant activities of clove basil (Ocimum gratissimum L.) grown in Nigeria. Int J Plant Res 2012;2:51-8.
Akinyemi KO, Mendie UE, Smith ST, Oyefolu AO, Coker AO. Screening of some medicinal plants used in south-west Nigerian traditional medicine for anti-Salmonella typhi activity. J Herb Pharmacother 2005;5:45-60.
Adebolu TT, Oladimeji SA. Antimicrobial activity of leaf extracts of Ocimum gratissimum on selected diarrhoea causing bacteria in southwestern Nigeria. Afr J Biotech 2005;4:682-4.
Matasyoh LG, Matasyoh JC, Wachira FN, Kinyua MG, Thairu MAW, Mukiama TK. Chemical composition and antimicrobial activity of the essential oil of Ocimum gratissimum L. growing in Eastern Kenya. Afr J Biotech 2007;6:760-5.
Ishiwu CN, Umenwanne CP, Obiegbuna JE, Uchegbu NN. In vitro assessment of the anti bacterial effect of extracts of Ocimum Gratissimum and Carica Papaya leaves. Int J Appl Sci Technol 2014;4:171-7.
Sharma SM, Bhadange DG. Antimicrobial potential of lamiaceae members. Int J Pharm Sci 2013;3:324-7.
Adesegun AS, Samuel FO, Anthony OB, Nurudeen OA. Antioxidant and inhibitory properties of essential oil of Ocimum gratissimum against extracellular protease of Escherichia Coli. Iosr J Pharm 2013;3:50-5.
Stanley MC, Ifeanyi OE, Chinedum OK, Chinenye ND. The antibacterial activity of leaf extracts of Ocimum gratissimum and Sida acuta. Int J Microbiol Res 2014;5:124-9.
Kpadonou KBG, Ladekan EY, Kpoviessi DS, Gbaguidi F, Yehouenou B, Quetin-Leclercq J, et al. Chemical variation of essential oil constituents of Ocimum gratissimum L. from Benin, and impact on antimicrobial properties and toxicity against Artemia salina leach. Chem Biodivers 2012;9:139-50.
Nguefack J, Budde BB, Jakobsen M. Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry. Lett Appl Microbiol 2004;39:395-400.
Dubey NK, Tiwari TN, Mandin D, Andriamboavonjy H, Chaumont Jean-Pierre. Antifungal properties of Ocimum gratissimum essential oil (ethyl cinnamate chemotype). Fitoterapia 2000;71:567–9.
Obot MJ, Aluyi HAS. Treatment of superficial mycoses with Ocimum gratissimum. Int J Infect Dis 2002;6:151.
Nwosu MO, Okafor JI. Preliminary studies of the antifungal activities of some medicinal plants against basidiobolus and some other pathogenic fungi. Mycoses 1995;38:191-5.
Braga FG, Lúcia M, Bouzada M, Fabri RL, deOMatos M, Moreira FO, et al. Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil. J Ethnopharmacol 2007;111:396–402.
Nakamura CV, Ishida K, Faccin LC, Filho BPD, Diógenes AG, Cortez DAG, et al. In vitro activity of essential oil from Ocimum gratissimum L. against four Candida species. Res Microbiol 2004;155:579-86.
Fokou JBH, Dongmo PMJ, Boyom FF, Menkem EZ, Bakargna-Via I, Tsague IFK, et al. Antioxidant and antifungal activities of the essential oils of Ocimum gratissimum from Yaoundé and Dschang (Cameroon). J Pharm Pharmacol 2014;2:257-68.
Lemos JdeA, Passos XS, Fernandes OdeFL, de Paula JR, Ferri PH, Souza LKH, et al. Antifungal activity from Ocimum gratissimum L. towards Cryptococcus neoformans. Mem Inst Oswaldo Cruz 2005;100:55-8.
Silva MR, Oliveira JGJr, Fernandes OF, Passos XS, Costa CR, Souza LK, et al. Antifungal activity of Ocimum gratissimum towards dermatophytes. Mycoses 2005;48:172-5.
Sethi S, Prakash O, Chandra M, Punetha H, Pant AK. Antifungal activity of essential oils of some Ocimum species collected from different locations of Uttarakhand. Indian J Nat Prod Resour 2013;4:392–7.
Mohr FBM, Lermen C, Gazim ZC, Gonçalves JE, Alberton O. Antifungal activity, yield, and composition of Ocimum gratissimum essential oil. Genet Mol Res 2017;16. Doi:10.4238/gmr16019542.
Faria TdeJ, Ferreira RS, Yassumoto L, de Souza JRP, Ishikawa NK, Barbosa AdeM. Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi. Braz Arch Biol Technol 2006;49:867-71.
Adjou ES, Kouton S, Dahouenon-Ahoussi E, Mohamed M, Soumanou MM, Sohounhloue DCK. Effect of essential oil from fresh leaves of Ocimum gratissimum L. on mycoflora during storage of peanuts in Benin. Mycotoxin Res 2013;29:29-38.
Bankole SA, Somorin YM. Antifungal activity of extracts of Ocimum gratissimum and Aframomum danielli against molds isolated from stored rice. 10th International Working Conference on Stored Product Protection. ed. Julius-Kühn-Archiv; 2010. p. 425.
Dambolena JS, Zunino MP, López AG, Rubinstein HR, Zygadlo JA, Mwangi JW, et al. Essential oils composition of Ocimum basilicum L. and Ocimum gratissimum L. from Kenya and their inhibitory effects on growth and fumonisin production by Fusarium verticillioides. Innovative Food Sci Emerging Technol 2010;11:410–4.
Fandohan P, Gbenou JD, Gnonlonfin B, Hell K, Marasas WF, Wingfield MJ. Effect of essential oils on the growth of Fusarium verticillioides and fumonisin contamination in corn. J Agric Food Chem 2004;52:6824-9.
Enikuomehin OA, Ikotun T, Ekpo EJ. Evaluation of ash from some tropical plants of Nigeria for the control of sclerotium rolfsii Sacc on wheat (Triticum aestivum L.). Mycopathologia 1998;142:81-7.
Nguefack J, Leth V, Amvam Zollo PH, Mathur SB. Evaluation of five essential oils from aromatic plants of cameroon for controlling food spoilage and mycotoxin-producing fungi. Int J Food Microbiol 2004;94:329-34.
Prakash B, Shukla R, Singh P, Mishra PK, Dubey NK, Kharwar RN. Efficacy of chemically characterized Ocimum gratissimum L. essential oil as an antioxidant and a safe plant based antimicrobial against fungal and aflatoxin B1 contamination of spices. Food Res Int 2011;44:385–90.
Nguefack J, Dongmo JBL, Dakole CD, Leth V, Vismer HF, Torp J, et al. Food preservative potential of essential oils and fractions from Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris against mycotoxigenic fungi. Int J Food Microbiol 2009;131:151–6.
Sessou P, Farougou S, Ahounou S, Hounnankpon Y, Azokpota P, Youssao I, et al. Comparative study of antifungal activities of six selected essential oils against fungal isolates from cheese wagashi in Benin. Pak J Biol Sci 2013;16:1751-7.
Jedlickova Z, Mottl O, Sery V. Antibacterial properties of the Vietnamese cajeput oil and ocimum oil in combination with antibacterial agents. J Hyg Epidemiol Microbiol Immunol 1992;3:303-9.
Orafidiya LO, Adesina JrSK, Igbeneghu OA, Akinkunmi EO, Adetogun GE, Salau AO. The effect of honey and surfactant type on the antibacterial properties of the leaf essential oil of Ocimum gratissimum Linn. against common wound-infecting organisms. Int J Aromather 2006;16:57–62.
Orafidiya LO, Oyedele AO, Shittu AO, Elujoba AA. The formulation of an effective topical antibacterial product containing Ocimum gratissimum leaf essential oil. Int J Pharm 2001;(1, Suppl 2):177-83.
Nguefack J, Tamgue O, Lekagne Dongmo JB, Dakole C, Leth V, Vismer H, et al. Synergistic action between fractions of essential oils from Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris against Penicillium expansum. Food Control 2012;23:377-83.
Iwalokun BA, Gbenle GO, Adewole TA, Smith SI, Akinsinde KA, Omonigbehin EO. Effects of Ocimum gratissimum L essential oil at subinhibitory concentrations on virulent and multidrug-resistant Shigella strains from Lagos, Nigeria. APMIS 2003;111:477-82.
Coutinho HD, Matias EF, Santos KK, Santos FA, Morais-Braga MF, Souza TM, et al. Modulation of the norfloxacin resistance in Staphylococcus aureus by Croton campestris A. and Ocimum gratissimum L. Biomedica 2011;31:608-12.
Silva MKdoN, Carvalho VRDeA, Matias EFF. Chemical profile of essential oil of Ocimum gratissimum L. and evaluation of antibacterial and drug resistance-modifying activity by gaseous contact method. Pharmacogn J 2016;8:4-9.
Biasi-Garbin RP, Otaguiri ES, Morey AT, da Silva MF, Morguette AEB, Lancheros CAC. Effect of eugenol against Streptococcus agalactiae and synergistic interaction with biologically produced silver nanoparticles. Evid Based Complementary Alternat Med 2015. http://dx.doi.org/10.1155/2015/861497.
Arrey TP, Okalebo FA, Ayong LS, Agbor GA, Guantai AN. Anti-malarial activity of a polyherbal product (Nefang) during early and established Plasmodium infection in rodent models. Malaria J 2014;13:456.