COMPARATIVE QUANTITATIVE PHYTOCHEMICAL ANALYSIS OF THE LEAVES OF SENNA ITALICA COLLECTED FROM DIFFERENT AREAS IN LIMPOPO PROVINCE, SOUTH AFRICA
DOI:
https://doi.org/10.22159/ijpps.2018v10i2.22950Keywords:
Medicinal plants, Phytochemical accumulation, Geographical location, Altitude, Quantitative phytochemical analysis, Senna italicAbstract
Objective: The current study was aimed at comparative quantitative phytochemical analysis of the leaves of S. italica collected from four districts in Limpopo province, South Africa, in order to establish whether geographical location have an effect on the accumulation of phytochemicals within the leaves of the plant species under study.
Methods: The leaves of S. italica were collected from four districts in Limpopo province of South Africa namely; Capricorn, Sekhukhune, Vhembe and Waterberg districts, dried, ground to powder and extracted using different organic solvents. The extracts of the leaf samples from different locations were subjected to quantitative phytochemical analysis for total phenolic content, total tannin content, total flavonoid content and total saponin content using spectrophotometric measurements. The resultant quantities were analysed for statistical differences.
Results: The leaf samples of S. italica from the four districts in Limpopo province showed significant differences (*p<0.05) in their phytochemical quantities, with main data expressed as mean±SD. Total phenolic content was in highest amounts in leaf samples from Waterberg district compared to samples from other districts. Total tannin content was in highest amounts in the Vhembe district leaf samples compared to samples from other districts. Total flavonoid content was in highest amounts in the leaf samples from Waterberg district compared to samples from other districts. Total saponin content was in highest amounts in the Vhembe district leaf samples compared to samples from other districts.
Conclusion: The findings of the study thus suggest that geographical location has an effect on the accumulation of phytochemicals in the leaves of S. italica.
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Ogundipe T, Ajayi GO. History and practice of traditional medicine and its challenges. In: Odugbemi T. editors. A Textbook of Medicinal Plants from Nigeria, Lagos: University of Lagos Press; 2008. p. 19-24.
Van Wyk BE, Van Oudsthoorn B, Gericke N. Medicinal plants of South Africa. Briza Publications (Pretoria); 1997.
Rahmani AH, Aly SM. Nigelia sativa and its active constituents thymoquinone shows pivotal role in the diseases prevention and treatment. Asian J Pharm Clin Res 2015;8:48-53.
Saxena M, Saxena J, Nema R, Singh D, Gupta A. Phytochemistry of medicinal plants. J Pharmacogn Phytochem 2013;1:168-82.
Kiranmayi P. Is bioactive compounds in plants acts as antinutritional factors? Int J Curr Pharm Res 2014;6:36-8.
Nchabeleng L, Mudau FN, Mariga IK. Effects of the chemical composition of wild bush tea (Athrixia phylicoides DC.) growing at locations differing in altitude, climate and edaphic factors. J Med Plants Res 2012;6:1662-6.
Pawson IG, Jest C. The high-altitude areas of the world and their cultures. In: Baker PT. editors. The Biology of High-Altitude Peoples. Cambridge: University Press (UK); 1978. p. 17-45.
Newman DJ, Cragg GM. Natural products as sources of new drugs over the 30 y from 1981 to 2010. J Nat Prod 2012;75:311-35.
Masoko P, Gololo SS, Mokgotho MP, Eloff JN, Howard RL, Mampuru LJ. Evaluation of the antioxidant, antibacterial and antiproliferative activities of the acetone extract of the roots of Senna italica (Fabaceae). ‎Afr J Tradit Complementary Altern Med 2010;7:138-48.
Lekganyane MA, Matsebatlela TM, Howard RL, Shai LJ, Masoko P. The phytochemical, antibacterial and antioxidant activity of five medicinal plants against the wound-infecting bacteria. Afr J Biotechnol 2012;11:13210-9.
Tshikalange TE, Meyer JJM, Husein AA. Antimicrobial activity, toxicity and the isolation of bioactive compounds from plants used to treat sexually transmitted diseases. J Ethnopharmacol 2005;96:515-9.
Tambe VD, Bhambar RS. Estimation of total phenol, tannin, alkaloid and flavonoids in Hibiscus tiliaceus Linn. Wood extracts. Res Rev: J Pharmacogn Phytochem 2014;2:41-7.
Senguttuvan J, Paulsamy S, Karthika K. Phytochemical analysis and evaluation of leaf and root parts of medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pac J Trop Biomed 2014;4:S359-67.
Inbathamizh L, Padmini E. Effect of geographical properties on the phytochemical composition and antioxidant potential of Moringa oleifera flowers. BioMedRx 2013;1:239-47.
Morris T, Stables M, Hobbs A, de Souza P, Colville-Nash P, Warner T, et al. Effects of low-dose aspirin on acute inflammatory responses in humans. J Immunol 2009;183:2089-96.
Ross IA. Medicinal plants of the world: Vol. 1: Chemical Constituents, Traditional and Medicinal uses. 2nd ed. New York: Springer-Science+Busines Media, LLC; 2003.
Mahdavi M, Jouri MH, Salehi MD, Motevalizadeh A. The effects of ecological factors of the site on quality and quantity of the essence from Phlomis cancellata Bunge in Mazandaran province (Iran). Int J Agric Crop Sci 2014;7:1478-84.
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