• Kiran Kemkar Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411 038, Maharashtra, India
  • Sathiyanarayanan L. Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411 038, Maharashtra, India
  • Arulmozhi Sathiyanarayanan Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411 038, Maharashtra, India
  • Kakasaheb Mahadik Bharati Vidyapeeth University, Poona College of Pharmacy, Pune 411 038, Maharashtra, India




6-shogaol, Mixed micelles, Factorial designs, Breast cancer


Objective: Ginger oleoresin (GO) plays an important role on the attenuation of complications associated to the cancer which is attributed to 6-shogaol (6-SGL). The major challenge in using 6-SGL for therapeutic applications is its poor aqueous solubility, low stability in GI and low bioavailability. Considering the potent anticancer nature of 6-SGL and its synergistic activity with other constituents in GO, there is a need to develop a suitable drug delivery system.

Methods: Thus in the present study, 6-SGL rich GO (6-SRGO) was incorporated into mixed micelles using phospholipid (Soya Lecithin) as a carrier. The prepared 6-SRGO loaded mixed micelles (6-SRGO-LMM) were characterized physically and chemically using Fourier transform infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC) and further evaluated for stability study, in vitro release study, in vitro cytotoxicity study and in vivo anticancer activity in comparison with 6-SRGO.

Results: The composition such as, drug content (86.27±1.56), encapsulation efficiency (81.55±1.05) and particle size (356.11±4.07) were optimized using 32 factorial design. FTIR and DSC study confirm that the 6-SGL from 6-SRGO was entrapped in the core of phospholipid by self-assembly method to form mixed micelles. The 6-SRGO-LMM exhibited significant in vitro (GI50-23.2 μg/ml) and in vivo anticancer activity in comparison with 6-SRGO.

Conclusion: We have developed and investigated mixed micelles composed of phospholipids (soya lecithin S80) and SCH as an effective nanocarrier for the delivery of a natural lipophilic anticancer bioactive 6-SGL from 6-SRGO.


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Kelly S, Kevin M, Aranee C, Luis A, Vondel R, Chen L, et al. Microencapsulation of ginger (Zingiber officinale) extract by spray drying technology. Food Sci Technol 2016;70:119-25.

Shukla Y, Singh M. Cancer preventive properties of ginger: a brief review. Food Chem Toxicol 2007;45:683–90.

Bothiraja C, Kapare H, Pawar A, Shaikh K. Development of plumbagin-loaded phospholipid–Tween® 80 mixed micelles: formulation, optimization, effect on breast cancer cells and human blood/serum compatibility testing. Ther Delivery 2013;4:1247–59.

Oriania V, Alvimb I, Consolia L, Molinac C, Pastored G, Hubingera M. Solid lipid microparticles produced by spray chilling technique to deliver ginger oleoresin: structure and compound retention. Food Res Int 2016;80:41–9.

Singh G, Kapoor Singh P, Heluani C, Lampasona M, Catalan C. Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale. Food Chem Toxicol 2008;46:3295–302.

Lawal T, Slover C, Lee V, Mahady G. Ginger (zingiber officinale roscoe, zingiberaceae) extract and 10-gingerol enhance the activity of clarithromycin against resistant helicobacter strains. Planta Med 2016;82:5.

Jain P, Das D, Singhai AK. Alternative herbal drugs used for treating hair disease. Asian J Pharm Clin Res 2016;9:110-2.

Karale P, Karale M. An overview on plants with anti-inflammatory potential. Int J Curr Pharm Res 2017;9:1-4.

Tyagi R, Sharma G, Jasuja ND, Menghani E. Indian medicinal plants as an effective antimicrobial agent. J Crit Rev 2016;3:69-71.

Chen H, Lishuang L, Soroka D, Warin R, Parks T, Hu Y, et al. Metabolism of [6]-shogaol in mice and in cancer cells. Drug Metab Dispos 2012;40:742–53.

Wu H, Hsieh M, Lo C, Liu C, Sang S, Ho C, et al. 6-Shogaol is more effective than 6-gingerol and curcumin in inhibiting 12-O-tetradecanoylphorbol 13-acetate-induced tumor promotion in mice. Mol Nutr Food Res 2010;54:1296-306.

Kim J, Lee S, Park H, Yang J, Shin T, Kim Y, et al. Cytotoxic components from the dried rhizomes of Zingiber officinale roscoe. Arch Pharm Res 2008;31:415-8.

Surh Y, Lee E, Lee J. Chemoprotective properties of some pungent ingredients present in red pepper and ginger. Mutat Res 1998;402:259–67.

Nikam A, Sathiyanarayanan L, Mahadik K. Mapping pharmaco-kinetic and tissue distribution profile of [6]-shogaol from ginger oleoresin. Int J Pharm Pharm Sci 2013;5:185-9.

Kumar P, Paul W, Sharma C. Green synthesis of gold nanoparticles with Zingiber officinale extract: Characterization and blood compatibility. Process Biochem 2011;46:2007-13.

Uthumpa C, Indranupakorn R, Asasutjarit R. Development of nanoemulsion formulations of ginger extract. Advs Appl Mater Electro Eng 2013;684:12-5.

Ratcharin N, Wongtrakul P, Indranupakorn R. Preparation of zingiber officinale extract loaded solid lipid nanoparticles. Biomat Appls 2012;506:389-92.

Akula NP. Preparation and evaluation of shampoo powder containing herbal ingredients. Asian J Pharm Clin Res 2015;8:266-70.

Pituanan BS, Surini S. Fast-disintegrating tablet formulation of ginger (zingiber officinale rosc.) extract using co-processed excipient of pre-gelatinized cassava starch-acacia gum. Int J Appl Pharm 2017;9:77-84.

Kulthe S, Inamdara N, Choudhari Y. Mixed micelle formation with hydrophobic and hydrophilic pluronic block copolymers: implications for controlled and targeted drug delivery. Colloids Surf B 2011;88:691–6.

Chiappetta D, Hocht C, Opezzo J. Intranasal administration of antiretroviral loaded micelles for anatomical targeting to the brain in HIV. Nanomedicine 2013;8:223–37.

Jones M, Leroux J. Polymeric micelles-a new generation of colloidal drug carriers. Eur J Pharm Biopharm 1999;48:101-11.

Yoncheva K, Calleja P, Agueros M. Stabilized micelles as delivery vehicles for paclitaxel. Int J Pharm 2012;436:258–64.

Gao H, Cao S, Chen C. Incorporation of lapatini b into lipoprotein-like nanoparticles with enhanced water solubility and antitumor effect in breast cancer. Nanomedicine 2013;8:1429–42.

Gao Z, Tian L, Hu J. Prevention of metastasis in a 4T1 murine breast cancer model by doxorubicin carried by folate conjugated pH-sensitive polymeric micelles. J Controlled Release 2011;152:84–9.

Li X, Zhang Y, Fan Y. Preparation and evaluation of novel mixed micelles as nanocarriers for intravenous delivery of propofol. Nanoscale Res Lett 2011;6:275.

Tong S, Xiang B, Dong D. Enhanced antitumor efficacy and decreased toxicity by self-associated docetaxel in phospholipid-based micelles. Int J Pharm 2012;434:413–9.

Muthu M, Kulkarni S, Liu Y. Development of docetaxel-loaded vitamin E TPGS micelles: formulation optimization, effects on brain cancer cells and biodistribution in rats. Nanomedicine 2012;7:353–64.

Saxena V, Hussain M. Poloxamer 407/TPGS mixed micelles for delivery of gambogic acid to breast and multidrug resistant cancer. Int J Nanomed 2012;7:713–21.

Nikam A, Sathiyanarayanan L, Mahadik K. Validation of reversed-phase high-performance liquid chromatography method for simultaneous determination of 6-, 8-, and 10-shogaol from ginger preparations. Int J Pharm Pharm Sci 2013;5:432-7.

Liu J, Zhao X. Design of self-assembling peptides and their biomedical applications. Nanomedicine 2011;6:1621–43.

Sathiyanarayanan L, Arulmozi S, Chidhambarnathan N. Anti carcinogenic activity of Leptadenia reticulataI against dalton’s ascitic lymphoma. Iran J Pharmacol Toxicol 2006;6:133–6.

Unnikrishnan M, Kuttan R. Tumor reducing and anti-carcinogenic activity of selected species. Cancer Lett 1990;51:85-9.

Jiang-nan Y, Yuan Z, Li W, Min P, Shan-shan T, Xia C, et al. Enhancement of oral bioavailability of the poorly water-soluble drug silybin by sodium cholate/phospholipid-mixed micelles. Acta Pharmacol Sin 2010;31:759–64.

Rupp C, Steckel H, Muller B. Mixed micelle formation with phosphatidylcholines: the influence of surfactants with different molecule structures. Int J Pharm 2010;387:120–8.

Prasad S, Giri A. Antitumor effect of cisplatin against murine ascites Dalton’s lymphoma. Indian J Exp Biol 1994;32:155-62.

Bhattarai S, Tran V, Duke C. Stability of [6]-gingerol and [6]-shogaol in simulated gastric and intestinal fluids. J Pharm Biomed Anal 2007;45:648–53.

Brigger I, Dubernet C, Couvreur P. Nanoparticles in cancer therapy and diagnosis. Adv Drug Delivery Rev 2002;54:631–51.

Tulini F, Souza V, Echalar-Barrientos A, Thomazini M, Pallone E, Favaro-Trindade C. Development of solid lipid microparticles loaded with a proanthocyanidin rich cinnamon extract (Cinnamomum zeylanicum): Potential for increasing anti-oxidant content in functional foods for diabetic population. Food Res Int 2016;85:10–8.

Koo O, Rubinstein I, Onyuksel H. Camptothecin in sterically stabilized phospholipid micelles: a novel nanomedicine. Nanomedicine 2005;1:77–84.

Mehta A, Arora N, Gaur S, Singh B. Acute toxicity assessment of choline by inhalation, intraperitoneal and oral routes in Balb/c mice. Regul Toxicol Pharmacol 2009;54:282–6.

Clarkson B, Burchenal J. Preliminary screening of antineoplastic drugs. Prog Clin Cancer 1965;1:625-9.

Price V, Greenfield R. Anemia in cancer. Adv Cancer Res 1958;5:199-200.

Hogland H. Hematological complication of cancer chemotherapy. Semin Oncol 1982;9:95-102.

Fenninger L, Mider G. Energy and nitrogen metabolism in cancer. Adv Cancer Res 1954;2:229-53.



How to Cite

Kemkar, K., S. L., A. Sathiyanarayanan, and K. Mahadik. “6-SHOGAOL RICH GINGER OLEORESIN LOADED MIXED MICELLES ENHANCES IN VITRO CYTOTOXICITY ON MCF-7 CELLS AND IN VIVO ANTICANCER ACTIVITY AGAINST DAL CELLS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 10, no. 1, Jan. 2018, pp. 160-8, doi:10.22159/ijpps.2018v10i1.23077.



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