ANTIFUNGAL ACTIVITY OF LIPASE MODIFIED FLAVONOIDS FROM CITRUS LIMETTA
Keywords:
Citrus limetta, Flavonoids, Lipase, Ester, AntifungalAbstract
Objective: The aim of the present study is to bring about enzymatic modification of flavonoids from Citrus limetta in order to increase their antifungal activity.
Methods: Methanolic extraction of flavonoids from citrus peels was carried out and their presence was confirmed by various tests. Crude extract of flavonoids was subjected to enzymatic modification by enzyme lipase in presence of acyl donor tributyrin in order to bring about esterification. Esterification was confirmed using IR spectroscopy and sodium hydroxide- phenolphthalein test. Antifungal activity of the treated sample and control sample were tested against Candida albicans and compared with fluconazole by Kirby Bauer disk diffusion method.
Results: Methanolic extract showed the presence of flavonoids on carrying out various test. While testing enzymatic modification, IR spectra did not show a significant difference between treated and control; however, enhanced esterification was confirmed by sodium hydroxide-phenolphthalein test. On carrying out antifungal activity, treated samples showed a 56.52% increase in zone of inhibition against Canidida albicans as compared to the control and was also found to be more in comparison to fluconazole.
Conclusion: The results signify that enzymatic modification increases the antifungal activity of flavonoids from Citrus limetta.
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References
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