ANTIOXIDANT, PHYTOCHEMICAL AND ANTIBACTERIAL ACTION OF HIMALAYAN MEDICINAL HERBS PERISTROPHE BICALYCULATA LEAVES EXTRACT AGAINST RESPIRATORY TRACT PATHOGENS
DOI:
https://doi.org/10.22159/ijpps.2018v10i3.23961Keywords:
Antibacterial activity, Agar well diffusion method, Twofold serial dilution method, Antioxidant, Phytomedicine, Respiratory tract pathogensAbstract
Objective: Evaluate the antioxidant, phytochemicals and antibacterial screening of leaves extracts of Peristrophe bicalyculata.
Methods: The antibacterial activity against the respiratory tract pathogens i.e., Staphylococcus aureus (MTCC 1144), Streptococcus pneumoniae (MTCC 655), Streptococcus pyogenes (MTCC 442), Pseudomonas aeruginosa (MTCC 2474) and Klebsiella pneumoniae (MTCC 4030) was examined by the method agar well diffusion and the minimum inhibitory concentration (MICs) was determined by the method of twofold serial dilution. Broad spectrum antibiotic erythromycin was used as positive control and dimethyl sulphoxide (DMSO) used as negative control. The qualitative method was adapted for phytoconstituents screening and antioxidant activity of plant extract was examined by DPPH free radical scavenging method.
Results: The results showed that the chloroform (CHF) extract has a higher degree of antibacterial potency then the other extract. The zone of inhibition showed by chloroform extract against tested bacteria ranged between 9.3±0.59 mm to 26.6±0.66 mm, respectively. MICs values were recorded between 6.25 mg/ml to 25 mg/ml against all the test organisms. Phytoconstituents analysis of P. bicalyculata extract exposed the presence of alkaloids, flavonoids, glycosides, steroids, saponins and tannins. The methanolic extract of P. bicalyculata % inhibition of DPPH radical is up to 86.33%. The P. bicalyculata (Methanolic extract) gives best antioxidant activity than another extract.
Conclusion: This investigation ropes a good answer to the use of P. bicalyculata as a natural antioxidant and in herbal medicine as a support for the development of new drugs and phytomedicine in the foundation for its use in remedial of respiratory infectious diseases.
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References
Srivastava J, Lambert G, Vietmeyer V. Medicinal plants: an expanding role in development. World Bank Technical Paper 2006;320−2. Doi:10.1596/0-8213-3613-4
Ghani A. In: Traditional Medicine. Jahangirnagar University Savar Dhaka; 1990. p. 15−9.
Gaur RD. Flora of the District Garhwal North-West Himalaya (with Ethno Botanical Uses). Transmedia Publication Srinagar; 1999. p. 234−5.
Ahmad I, Mehmood Z, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol 1998;62:183−3.
Aboaba OO, Smith SI, Olude FO. Antibacterial effect of edible plant extract on Escherichia coli. Pak J Nutr 2006;5:325−7.
Evans WC. Trease and evans pharmacognosy. Harcourt Brace Company India 1996;15:341−1.
Sheng ZW, Gao JH, Bil Y, Zhang WM, Dou HT, Jin ZQ. Antioxidant properties of the banana flower of two cultivars in China using 2,2-diphenyl-1-picrylhydrazyl (DPPH) reducing power 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate (ABTS) and inhibition of lipid peroxidation assays. Afr J Biotechnol 2011;10:4470−7.
Janakiraman N, Johnson M, Sahaya SS. G C-M S analysis of bioactive constituents of Peristrophe bicalyculata (Retz.) nees (Acanthaceae). Asian Pac J Trop Biomed; 2012. p. S46−9.
Nabere O, Hilou A, Guenne S, Konate K, Zerbo P, Meda Nag-Tiero R, et al. Antibacterial and phytochemical studies of three Acanthaceae species used in Burkina Faso traditional medicine. J Appl Pharm Sci 2013;3:49−5.
Giwa OE, Seyifunmi OE, Adewumi BL, Adebote VT, Aladejimokun AO. Screening of antimicrobial ethanolic extract of Peristrophe bicalyculata. Ethnobot Leaflets 2010;14:766−3.
Abdulazeez MA, Ibrahim AD, Ayo JO, Carvalho LGC. Anticancer activities of extracts of Peristrophe bicalyculata (RETZ) NEES. Rom Biotechnol Lett 2013;18:7995−8006.
Selvamohan TV, Ramadas S, Kishore SS. Antimicrobial activity of selected medicinal plants against some selected human pathogenic bacteria. Adv Appl Sci Res 2012;3:3374−1.
Johnley IR, Joshi P. In vitro antioxidant activity of various extracts of the whole plant of Peristrophe bicalyculata forssk. Int J Pharm Drug Anal 2014;2:705−9.
Krishnamoorthy K, Krishnaswamy T, Subramaniam P, Sellamuthu M. Quantification of phytochemicals and in vitro antioxidant potential of various solvent extracts of certain species of Acanthaceae. ‎Int J Green Pharm 2014;8:58−4.
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