PRELIMINARY PHYTOCHEMICAL AND ANTIBACTERIAL STUDIES ON LEAF EXTRACTS OF ALTERNANTHERA BRASILIANA (L.) KUNTZE
Keywords:
Antibacterial activity, Alternanthera brasiliana, Ecoli, Phytochemical, FlavanoidAbstract
Objectives: The present study was to study the phytochemical and antibacterial properties of Alternanthera brasiliana leaf extracts using three different solvents (hexane, chloroform and methanol) against human pathogenic microorganism.
Methods: Preliminary phytochemical screening of leaf extracts (in different solvents) of A. brasiliana were qualitatively tested using standard procedures. Thin Layer Chromatography (TLC) was performed to detect the phytochemical compounds present in A. brasiliana. Antibacterial activity of crude extract was determined by disc diffusion method. Minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were also performed for the hexane extract.
Results: The preliminary qualitative phytochemical screening of hexane extracts revealed the presence of alkaloids, phenols, flavonoids, saponins, tannins, phytosterols and carbohydrates. In chloroform and methanolic extracts, all the tested phytochemicals were present except flavanoids and saponins. Alkaloids were not present in the chloroform extract. TLC analysis revealed the presence of seven compounds with Rf values ranging from 0.173 to 0.933. Promising antibacterial activity was observed in the hexane extract with an inhibition zone of 22 mm against E. coli. Chloroform and methanolic extracts showed negligible antibacterial activities.
Conclusion: The results revealed that the hexane leaf extract of A. brasiliana alone exhibited potential antibacterial activity specifically against pathogenic E. coli (MTCC 729).
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Karaman L, Sahin F, Gulluce M, Ogutcu H, Sngul M and Adiguzel A. Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. J Ethnopharmacol 2003; 85: 231-5.
Schinor EC, Salvador MJ, Ito IY and Dias DA. Evaluation of the antimicrobial activity of crude extracts and isolated constituents from Chresta scapigera. Brazilian J Microbiol 2007; 38: 145-9.
Chandran RP, Manju S, Vysakhi MV, Shaji PK, Nair GA. In vitro antimicrobial activities of Hygrophila schulli (Buch.-Ham) leaf and root extracts against clinically important human pathogens. Biomed Pharmacol J 2013; 6: 421-8.
Wood-Sheldon J, Balick MJ, Laird SA. Medicinal plants: can utilization and conservation coexist? The New York Botanical Garden, Bronx, New York, USA.
Nino J, Navaez DM, Mosquera OM, Correa YM. Antibacterial, antifungal and cytotoxic activities of eight Asteraceae and two Rubiaceae plants from Colombian biodiversity. Brazilian J Microbiol 2006; 37: 566-70.
Macedo AF, Lage CL, Esquibel MA, de Souza MM, Da Silva KL, Niero R, et al. Preliminary phytochemical and pharmacological studies on plantlets of Alternanthera brasiliana cultured under different spectral quality of lights. J Acta Farm Bonaerense 2004; 23: 515-9.
Brochado CO, Almeida AP, Barreto BP, Costa LP, Ribeiro LS, da C. Pereira RL, et al. Flavonol robinobiosides and rutinosides from Alternanthera brasiliana (Amaranthaceae) and their effects on lymphocyte proliferation in vitro. J Braz Chem Soc 2003; 14: 449-51.
Sanoko R, Speranza G, Pizza C, De Tommasi N. Triterpene saponins from Alternanthera repens. J Phytochem 1999; 51: 1043-7.
Hosamani KM, Ganjihal SS, Chavadi DV. Alternanthera triandra seed oil: A moderate source of ricinoleic acid and its possible industrial utilization. J Indus Crop Prod 2004; 19: 133-6.
Shridhar R, Gollamudi L. Lipid classes, fatty acids, and tocopherols of leaves of six edible plant species. J Agric Food Chem 1993; 41: 61-3.
Bing-Nan Z, Gabor B, Cordell GA. Alternanthin, A C- glycosylated flavonoids from Alternanthera philoxeroides. J Phytochem 1988; 27: 3633-6.
Guerra RN, Pereira HA, Silveira LM, Olea RS. Immunomodulatory properties of Alternanthera tenella Colla aqueous extracts in mice. Braz J Med Bio Res 2003; 36: 1215- 9.
Fang JB, Duan HQ, Zhang YW, Yoshihisa T. Antitumor constituent from Alternanthera hiloxeroides. J Asian Nat Prod Res 2007; 9: 511-5.
Mesa J, Blair S, Saez J, Correa A, Carmona J. In vitro antimalarial evaluation of the plants Alternanthera lanceolata and Pollalesta discolor. J Anales De Quimica 1998; 94: 67-70.
Zavala MA, Perez C, Vergas R, Perez Z. Antidiarrhoeal activity of Waltheria americana, Commelina coelestis and Alternanthera repens. J Ethnopharmacol 1998; 61: 41-7.
Agra MF, Baracho GS, Nurit K, Brasilio IJLD, Coelho VPM. Medicinal and poisonous diversity of the flora of Cariri Paraibano†Brazil. J Ethnopharmacol 2007; 111: 383-95.
Brain KR, Turner TD. Wright-Scientechnica. 1st Ed. Bristol: Practical evaluation of phytopharmaceuticals 1975.
Bauer AW, Kirby WMM, Sherri JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966; 45: 493-6.
Wayne PA. National Committee for Clinical Laboratory Standards 2000. M7-A5.p.
Wink M. Phytochemical diversity of secondary metabolites. J Encyclopedia of Plant and Crop Sci 2004; 915-9.
Banerji A, Chadha MS, Malshet VG. Isolation of 5-hydroxy-36-73’4’-pentamethoxy flavone from Vitex negundo. J Phytochem 1969; 8: 511-2.
Manju S, Chandran RP, Shaji PK, Nair GA. In vitro free radical scavenging potential of Acorus calamus L. rhizome from Kuttanad wetlands Kerala, India. Int J Pharm Pharm Sci 2013; 5: 376-80.
Chandran RP, Manju S, Vysakhi MV, Shaji PK, Nair GA. Antibacterial and antifungal activities of Thespesia populnea leaf extracts against human pathogens. Int J Pharm Tech Res 2014; 6: 290-7.