IN VITRO ANTIOXIDANT AND IN VIVO ANTI-INFLAMMATORY ACTIVITY OF THE AERIAL PART OF BLUMEA ERIANTHA DC
DOI:
https://doi.org/10.22159/ijpps.2018v10i7.25588Keywords:
Blumea eriantha DC, Anti-inflammatory, AntioxidantAbstract
Objective: Objective of the present study was to carry out in vivo anti-inflammatory and in vitro antioxidant activity of methanol extract of aerial part of the Blumea eriantha DC belonging to family Asteraceae.
Methods: The shade dried aerial part of B. eriantha (0.5 kg) was powdered and extracted with methanol (1.5 x 3L) at room temperature (24h x 3). After filtration combined all the three extracts and were concentrated on rotary evaporator under reduced pressure at 40 °C, thereby providing crude methanol extract which was subsequently employed for further studies. Anti-inflammatory effect was studied by carrageenan-induced paw edema model in rats at dose level 100, 200, and 400 mg/kg. Acute oral toxicity study and in vitro antioxidant potential of the extract was also studied. The in vitro antioxidant activity of methanol extract of aerial part of Blumea eriantha DC was evaluated against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) and hydroxyl (OH) radicalscavenging and reducing power assays.
Results: The results indicate that methanol extract of Blumea eriantha (BEME, 400 mg/kg) exhibited significant inhibition (p<0.001) of increase in paw edema at 5th h. IC50 value of BEME showed significant antioxidant activity. The extract exhibits promising free radical scavenging effect of DPPH, H2O2, OH and reducing power in a dose-dependent manner up to 100µg/ml concentration while the reference standard Ascorbic acid demonstrated more scavenging potential than the methanol extract of Blumea eriantha The methanol extract was found to be safe at the dose of 2000 mg/kg.
Conclusion: The results of the experimental study confirmed that methanol extract of Blumea eriantha DC possesses significant anti-inflammatory and antioxidant activity.
Downloads
References
Honmore V, Kandhare A, Kadam P, Bodhankar S. Isolates of Alpinia officinarum Hance as COX-2 inhibitors: evidence from anti-inflammatory, antioxidant and molecular docking studies. Int Immunopharmacol 2016;33:8-17.
Bihani GV, Rojatkar SR, Bodhankar SL. Investigation of in vivo analgesic and anti-inflammatory activity in rodents and in vitro antioxident activity of extracts of whole plant of Cyathocline purpurea. Int J Pharm Pharm Sci 2014;6:492-8.
Bhagyasri Y, Lavakumar M, Divya Sree MS, Ashok Kumar CK. An overview on anti-inflammatory activity of Indian herbal plants. Int J Res Pharm Nano Sci 2015;4:1-9.
Pattewar AM, Dawalbaje AB, Gundale PB, Kavtikar PG, Yerawar PP, et al. Phytochemistryical and anthelmintic studies on Blumea lacera burm. f. Indo Global J Pharm Sci 2012;2:390-6.
Singh UP, Parthasarathy R. Comparative antidiarrhoel activity of ethanolic extract of root of Blumea lacera and Blumea eriantha DC on experimental animals. J Pharm Biomed Sci 2012;17:1-4.
Khurshid J, Sukalyan K, Abdul B. Evaluation of antimicrobial and cytotoxic activities of the mehanolic and petroleum ether extract of Blumea lacera Burm. f in Bangladesh. J Pharmacogn Phytochem 2014;2:104-8.
Gore M, Desai NS. Characterization of phytochemicals and evaluation of anti-cancer potential of Blumea eriantha DC. Physiol Mol Biol Plants 2014;20:475-86.
OECD, Test No. 425: Acute Oral Toxicity: Up-and-down Procedure. OECD Guidelines for the Testing of Chemicals, Section 4: Health Effects, OECD Publishing, Paris, OECD Publishing; 2008.
Kandhare AD, Bodhankar SL, Mohan V, Thakurdesai PA. Acute and repeated dose (28 d) oral toxicity study of glycosides based standardized fenugreek seed extract in laboratory mice. Regul Toxicol Pharmacol 2015;72:323-34.
Kandhare AD, Bodhankar SL, Mohan V, Thakurdesai PA. Acute and repeated doses(28 d) oral toxicity study of Vicenin-1, a flavonoid glycoside isolated from fenugreek seeds in laboratory mice. Regul Toxicol Pharmacol 2016;81:522-31.
Ruikar AD, Khatiwora E, Ghayal N, Misar A, Mujumdar A, Puranik V, et al. Studies on aerial parts of Artemisia pallens wall for phenol, flavonoid and evaluation of antioxidant activity. J Pharm Bioallied Sci 2011;3:302-5.
Ganapaty S, Chandrashekhar V, Chitme H, Narsu ML. Free radical scavenging activity of gossypin and nevadensin: an in vitro evaluation. Indian J Pharm 2007;39:281-3.
Al-Saghir J, Al-Ashi R, Salloum R, Saliba NA, Talhouk RS, FR Homaidan FR. Anti inflammatory properties of salograviolide a purified from lebanese plant Centaurea ainetensis. BMC Complement Altern Med 2009;9:36-5.
Kamble H, Kandhare A, Bodhankar S, Mohan V, Thakurdesai P. Effect of low molecular weight galactomannans from fenugreek seeds on animal models of diabetes mellitus. Biomed Aging Pathol 2013;3:145-51.
Honmore V, Kandhare A, Zanwar A, Rojatkar S, Bodhankar S, Natu A. Artemisia pallens alleviates acetaminophen induced toxicity via modulation of endogenous biomarkers. Pharm Biol 2015;53:571-81.
Kandhare A, Raygude K, Ghosh P, Bodhankar S. The ameliorative effect of fisetin, a bioflavonoid, on ethanol-induced and pylorus ligation-induced gastric ulcer in rats. Int J Green Pharma 2011;5:236-43.
Ghule AE, Kandhare AD, Jadhav SS, Zanwar AA, Bodhankar SL. Omega-3-fatty acid adds to the protective effect of flax lignan concentrate in pressure overload-induced myocardial hypertrophy in rats via modulation of oxidative stress and apoptosis. Int Immunopharmacol 2015;28:751-63.
Gosavi TP, Ghosh P, Kandhare AD, Kumar VS, Adil M, Rajmane AR, et al. Therapeutic effect of H. pylori nosode, a homeopathic preparation in healing of chronic H. pylori infected ulcers in laboratory animals. Asian Pac J Trop Dis 2012;2:S603-S11.
Adil M, Visnagri A, Kumar VS, Kandhare AD, Ghosh P, Bodhankar S. Protective effect of naringin on sodium arsenite induced testicular toxicity via modulation of biochemical perturbations in experimental rats. Pharmacologia 2014;5:222-34.
Adil M, Kandhare AD, Ghosh P, Venkata S, Raygude KS, Bodhankar SL. Ameliorative effect of naringin in acetaminophen-induced hepatic and renal toxicity in laboratory rats: role of FXR and KIM-1. Ren Fail 2016;38:1007-20.
Goswami S, Kandhare AD, Zanwar AA, Hegde MV, Bodhankar SL, Shinde S, et al. Oral L-glutamine administration attenuated cutaneous wound healing in Wistar rats. Int Wound J 2016;13:116-24.
Visnagri A, Kandhare AD, Kumar VS, Rajmane AR, Mohammad A, Ghosh P, et al. Elucidation of ameliorative effect of Co-enzyme Q10 in streptozotocin-induced diabetic neuropathic perturbation by modulation of electrophysiological, biochemical and behavioral markers. Biomed Aging Pathol 2012;2:157-72.
Tambewagh UU, Kandhare AD, Rojatkar SR. Anti-inflammatory and antioxidant potential of guaianolide isolated from Cyathocline purpurea: role of COX-2 inhibition. Int Immunopharmacol 2017;52:110-9.
Saraswathi KY, Muthal A, Kandhare A, Rojatkar S, Bodhankar S. Study of methanolic extract of Artemisia pallens wall on endurance of laboratory animals. Pharmacologia 2014;5:298-309.
Bhilare NV, Dhaneshwar SS, Sinha AJ, Kandhare AD, Bodhankar SL. Novel thioester prodrug of N-acetylcysteine for odor masking and bioavailability enhancement. Curr Drug Delivery 2016;13:611-20.
Ghaisas M, Navghare V, Takawale A, Zope V, Deshpande A. In vitro antioxidant activity of Tectona grandis Linn. Pharmacologyonline 2008;3:296-5.
Patil M, Kandhare A, Bhise S. Pharmacological evaluation of ameliorative effect of aqueous extract of Cucumis sativus L. fruit formulation on wound healing in Wistar rats. Chronicles Young Sci 2011;2:207-13.
Chanda S, Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: an overview. Afr J Microbiol Res 2009;3:981-96.
Oktay M, Gülçin I, Küfrevioglu OI. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. Food Sci Technol 2003;36:263-71.
Masferrer JL, Zweifel BS, Manning PT, Hauser SD, Leahy KM, Smith WG, et al. Selective inhibition of inducible cyclooxygenase2 in vivo anti-inflammatory and non-ulcerogenic. Proc Natl Acad Sci USA 1994;91:3228-32.
Smith JA. Neutrophils, host defense, and inflammation: a double-edged sword. J Leukoc Biol 1994;56:672-86.
Published
How to Cite
Issue
Section
