HYDROALCOHOLIC EXTRACTION OF SHOOT CULTURES FROM NOTHAPODYTE NIMMONIANA AND ITS ANTI-PROLIFERATIVE ANALYSIS

Arun Sharma; Ankita Rajata; Udayabanu Malairaman; Hemant Sood

doi:10.22159/ijpps.2018v10i6.25726

Authors

Arun Sharma Department of Pharmacy, Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, India 173234
Ankita Rajata Department of biotechnology and bioinformatics, Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, India 173234
Udayabanu Malairaman Department of Pharmacy, Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, India 173234
Hemant Sood Department of biotechnology and bioinformatics, Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, India 173234

DOI:

https://doi.org/10.22159/ijpps.2018v10i6.25726

Keywords:

Nothapodytes nimmoniana, Phytochemical, Plant tissue Culture, Anti-cancer activity, Camptothecin, HPLC

Abstract

Objective: The important restraints in plant-derived medicine are the convenience of active composites which depends on diverse topographical situations, active compound build-up pattern, environmental circumstances and genetic makeup of the plant. The presented work was aimed to describe an efficient technique for fast propagation of Nothapodytes nimmoniana which is an imperative source of Camptothecin.

Methods: Multiple shoots were successfully regenerated from the explants N. nimmoniana using Murashige and Skoog (MS) medium accompanied with IBA+KN+GA3 (1:3:2) mg/l. the hydro-alcoholic extract was obtained by soxhlet extraction for field grown roots (fgRE), shoots (fgSE) and in vitro plantlets (ivPL) and further subjected to phytochemicals analysis. HPLC analysis was performed to determine the percentage of camptothecin (CAM). Anti-proliferative studies were carried out followed by Acridine orange/Ethidium bromide staining. Extracts were also estimated for reactive nitrogen species (RNS) and reactive oxygen species (ROS).

Results: Preliminary qualitative chemical analysis of the extracts displayed the presence of alkaloids, carbohydrates, steroids, saponins, terpenoids and phenolics compounds. HPLC analysis revealed the presence of CAM in all the extracts. ivPL exhibited lowest anti-proliferative activity in contrast to other extract and percentage of ROS and RNS was also less. Although ivPL did not exhibited prominent anti-tumour effect, but in vitro accumulation of CAM in 2-months old shoots provides an alternative resource to 5 y-old field grown plants.

Conclusion: This work delivers a potential to lead to further increase the potency of CAM in in vitro shoots of two months old as an alternative to the field grown plants for treating cancers.

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Author Biography

Arun Sharma, Department of Pharmacy, Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, India 173234

Department of Pharmacy, Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, India-173234.

References

Ramesha BT, Amna T, Ravikanth G, Gunaga RP, Vasudeva R, Ganeshaiah KN, et al., Prospecting for camptothecines from Nothapodytes nimmoniana in the Western Ghats, South India: identification of high-yielding sources of camptothecin and new families of camptothecines. J Chromatog Sci 2008;46:362-8.

Shaanker UR, Ramesha BT, Ravikanth G, Gunaga R, Vasudeva R, Ganeshaiah KN. Chemical profiling of Nothapodytes nimmoniana for camptothecin, an important anticancer alkaloid: towards the development of a sustainable production system. Bioactive molecules and medicinal plants. Springer-Verlag, Berlin and Heidelberg; 2008. p. 197-213.

Dancey J, Eisenhauer E. Current perspectives on camptothecins in cancer treatment. Br J Cancer 1996;74:327-38.

Moertel CG, Schutt AJ, Reitemeier RJ, Hahn RG. Phase II study of camptothecin (NSC-100880) in the treatment of advanced gastrointestinal cancer. Cancer Chemother Rep 1972;56:95-101.

Manikandan M, Kannan K. Study on in vivo release and in vivo absorption of camptothecin-loaded polymeric nanoparticles: level a in vitro-in vivo correlation. Asian J Pharm Clin Res 2016;9:71-4.

Srivastava V, Negi AS, Kumar JK, Gupta MM, Khanuja SP. Plant-based anticancer molecules: a chemical and biological profile of some important leads. Bio Med Chem 2005;13:5892-908.

Yamazaki Y, Sudo H, Yamazaki M, Aimi N, Saito K. Camptothecin biosynthetic genes in hairy roots of Ophiorrhiza pumila: cloning, characterization and differential expression in tissues and by stress compounds. Plant Cell Physiol 2003;44:395-403.

Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol 2005;100:72-9.

Haisel D, Hofman P, Vagner M, Lipavska H, Ticha I, Schafer C, et al. Ex vitro phenotype stability is affected by in vitro cultivation. Bio Plant 2001;44:321-4.

Rajta A, Popli D, Kotvi P, Sood H. Optimization of culture conditions for the production and germination of artificial seed in an important medicinal plant, nothapodytes nimmoniana. Int J Innov Res Sci Eng 2017;3:70-7.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plants 1962;15:473-97.

Shanthi G, Saridha D, Mariappan V. Pharmacognostical studies on morinda tinctoria. Roxb. Int J Pharm Pharm Sci 2012;4:636-8.

Lokesh P, Balasubramanya S, Anuradha M. Cost effective quantification of camptothecin and a comparative study of its content in Nothapodytes foetida and Ophiorrhiza mungos sourced from selected geographical locations. Ori Pharma Experiment Med 2014;14:87-92.

Aswini A, Soundhari C. Production of camptothecin from endophytic fungi and characterization by high-performance liquid chromatography and anticancer activity against colon cancer cell line. Asian J Pharm Clin Res 2018;11:166-70.

Mokhtar MM, Shaban HM, Hegazy ME, Ali SS. Evaluating the potential cancer chemopreventive efficacy of two different solvent extracts of seriphidium herba-alba in vitro. Bull Facult Pharma Cairo University 2017;1:195-201

Sharma A, Mehta V, Parashar A, Malairaman U. Combinational effect of paclitaxel and clotrimazole on human breast cancer: proof for synergistic interaction. Synergy 2017;5:13-20.

Wiseman H, Halliwell B. Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer. Biochem J 1996;313:17-29.