POTENTIAL CYTOTOXIC DRUG EFFECTS OF SECONDARY METABOLITES DERIVED FROM SELECTED MEDICINAL PLANTS OF SAVANADURGA FOREST IN KARNATAKA

Authors

  • Ashwatha Reddy Ravikumar Dept. of P. G. Studies & Research in Biotechnology & Bioinformatics, Kuvempu University, Shankaraghatta 577451, Karnataka, India. Nitya Genome Technologies Pvt. Ltd., Bangalore 560003, Karnataka, India
  • Varun Madgaonkar Dept of Biotechnology, PES Institute of Technology, Bangalore 560085, Karnataka, India
  • R. T. VENKATESHA Department of Biotechnology, Dayananda Sagar College of Biological Sciences, Bangalore 560078, Karnataka, India
  • BHARATHI R. Department of Computer Science Engineering, BMS Institute of Technology, Bangalore 560064
  • V. Krishna Murthy Dept of Biotechnology, PES Institute of Technology, Bangalore 560085, Karnataka, India

Keywords:

Brine Shrimp Lethality Assay, Potato Crown Gall Tumor Inhibition Assay, Kirganelia reticulata, Camptothecin, Catharanthus roseus, Withania somnifera, cytotoxicity

Abstract

Objective: To evaluate the potential cytotoxic and antitumor activities of secondary metabolites of selected medicinal plants of Savanadurga forest, Karnataka.

Methods: The soxhlet extracted crude methanolic leaf extracts of nineteen medicinal plants were assessed for their potential cytotoxic and antitumor activities by brine shrimp lethality assay and potato tumor inhibition assay at 100μg/ml respectively.

Results: Kirganelia reticulata and Cissus quadrangularis showed highest cytotoxicity while Flacourtia indica failed to show any inhibitory activity in brine shrimp lethality assay. Kirganelia reticulata exhibited 100% antitumor activity while Albizzia amara failed to show any antitumor activity as tested by crown gall tumor inhibition assay.

Conclusion: Both brine shrimp lethality assay and potato tumor inhibition assay indicated that Kirganelia reticulata seems to be the best anticancer plant.

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References

Duke JA, Vasquez R. Amazonian Ethnobotanical Dictionary. CRS Press: Boca Raton, FL, USA; 1994.

Schultes RE, Raffauf RF. The healing forest: medicinal and toxic plants of the northwest Amazonia. Portland, OR: Dioscorides Press; 1990.

Farnsworth NR. Screening plants for new medicines. In: Biodiversity (Ed E. O. Wilson). National Academy Press, Washington, DC; 1998.

Murali KS, Kavitha A, Harish RP. Spatial patterns of tree and shrub species diversity in Savanadurga state forest, Karnataka. Curr Sci 2003;84(6):808-13.

Nick A, Rali T, Sticher O. Biological screening of traditional medicinal plants from Papua New Guinea. J Ethnopharm 1995;49:147-56.

Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 1982;45:31-4.

Silva TMS, Nascimento RJB, Batista MM, Agra MF, Camara CA. Brine shrimp bioassay of some species of Solanum from Northern Brazil. Brazil J Pharmacol 2007;17:35-8.

Jamil M, Mirza B, Yasmeen B, Khan MA. Pharmacological activities of selected plant species and their phytochemical analysis. J Med Plant Res 2012;6(37):5013-22.

Mirza SH. Minimizing antibiotic resistance–is there a way forward? Infec Dis J Pak 2007;16(3):75-9.

Pimenta LPS, Pinto GB, Takahashi JA, Silva LGF, Boaventura MAD. Biological screening of annonaceous brazilian medicinal plants using Artemia salina (Brine Shrimp Test). Phytomed 2003;10:209-12.

McLauglin JL, Chang CJ, Smith DL. Simple bench-top bioassays (brine shrimp and potato discs) for the discovery of plant antitumor compounds. In: Human Medicinal Agents from Plants. Kinghorn AD and Balandin MF (Eds.) ACS Symposium 534, American Chemical Society, Washington, DC; 1993. p.112-37.

Ashan U, Ansari FL, Ihsan-ul-Haq, Nazir S, Mirza B. Combinatorial synthesis, lead identification, and antitumor study of a chalcone-based positional-scanning library. Chem Biodiv 2007;4:203-14.

Islam MS, Rahman MM, Rahman MA, Qayum MA, Aslam MF. In vitro evaluation of Croton bonplandianum Baill as potential antitumor properties using Agrobacterium tumefaciens. J Agr Technol 2010;6(1):79-86.

Shruthi SD, Rajeshwari A, Govardhan Raju K, Pavani A, Vedamurthy AB, Ramachandra YL. Phytochemical and antioxidant analysis of leaf extracts from Kirganelia reticulata Baill. Int J Pharm Pharm Sci 2012;4(3):608-12.

Pors K, Paniwnyk Z, Teesdale-Spittle P, Plumb JA, Willmore E, Austin CA, et al. Alchemix: a novel alkylating anthraquinone with potent activity against anthracycline-and cisplatin-resistant ovarian cancer. Mol Cancer Ther 2003;2:607-10.

Gali-Muhtasib HU, Younes IH, Krchesy JJ, El-Sabban ME. Plant tannins inhibit the induction of aberrant crypt foci and colonic tumors by 1, 2-dimethylhydrazine in mice. Nutr Cancer 2001;39(1):108-16.

Batra P, Sharma AK. Anti-cancer potential of flavonoids: recent trends and future perspectives. 3Biotech 2013;3(6):439-59.

Published

01-10-2014

How to Cite

Ravikumar, A. R., V. Madgaonkar, R. T. VENKATESHA, B. R., and V. K. Murthy. “POTENTIAL CYTOTOXIC DRUG EFFECTS OF SECONDARY METABOLITES DERIVED FROM SELECTED MEDICINAL PLANTS OF SAVANADURGA FOREST IN KARNATAKA”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 10, Oct. 2014, pp. 238-41, https://journals.innovareacademics.in/index.php/ijpps/article/view/2607.

Issue

Vol 6, Issue 10, 2014

Section

Original Article(s)