COMPARATIVE STUDY OF THE ANTINOCICEPTIVE ACTION OF AMITRIPTYLINE WITH FLUOXETINE AND EVALUATION OF THEIR PROBABLE MECHANISM OF THIS ACTION IN ALBINO MICE
DOI:
https://doi.org/10.22159/ijpps.2018v10i11.27706Keywords:
Antinociceptive, Amitriptyline, Fluoxetine, Morphine, Naloxone, YohimbineAbstract
Objective: The aim of our study was to compare the anti-nociceptive action of amitriptyline with fluoxetine and evaluation of their probable mechanism of anti-nociceptive action by observing their individual interactions with morphine, naloxone, yohimbine, and ondansetron.
Methods: Albino mice weighing 25-35 grams were taken and divided into 12 groups. Group A-Control(distilled water), Group B-amitriptyline 20 mg/kg, Group C-fluoxetine 20 mg/kg, Group D-morphine 5 mg/kg, Group E-amitriptyline 20 mg/kg+ morphine 5 mg/kg, Group F-amitriptyline 20 mg/kg+ naloxone 3 mg/kg, Group G-amitriptyline 20 mg/kg+ yohimbine 2 mg/kg, Group H-amitriptyline 20 mg/kg+ ondansetron 0.1 mg/kg, Group I-fluoxetine 20 mg/kg+morphine 5 mg/kg, Group J-fluoxetine 20 mg/kg+ naloxone 3 mg/kg, Group K-fluoxetine 20 mg/kg+ yohimbine 2 mg/kg and Group L-fluoxetine 20 mg/kg+ ondansetron 0.1 mg/kg. Hot plate method and acetic acid writhing test were used to assess central and peripheral analgesic activity respectively.
Results: Both the amitriptyline and fluoxetine-treated animals showed significantly increased reaction time in a hot plate (p<0.05) and a significant decrease in the number of wriths in acetic acid writhing test (p<0.05), when compared with control. Animals in amitriptyline group showed significantly higher reaction time and less number of wriths when compared to fluoxetine group. Morphine increased, while naloxone, yohimbine and ondansetron decreased the reaction time in a hot plate. In the acetic acid writhing test, a number of wriths significantly decreased when co-treated with morphine and increased when co-treated with naloxone, yohimbine and ondansetron.
Conclusion: It is concluded that amitriptyline is a better antinociceptive agent than fluoxetine. Their central and peripheral mechanism of antinociception both involve opioidergic, serotonergic and noradrenergic pathway.
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