PHYTOCHEMICAL SCREENING AND IN VITRO ANTIBACTERIAL ASSESSMENT OF DIFFERENT INDIGOFERA ARRECTA SOLVENT EXTRACTS AGAINST SELECTED PATHOGENIC MICROORGANISMS
Keywords:
Phytochemical, Antibacterial, Plants, Indigo feraarrecta, MedicineAbstract
Objective: The current study was done to analyze qualitatively the presence of pharmacological compounds and the antibacterial activity of different extracts of Indigo feraarrecta.
Methods: The plant was extracted using hydromethanolic solvent. The extracted compounds were dissolved in different solvents according to their polarity and then concentrated using a rotar vapor with a water bath at 40oC. The antibacterial assessment was done using well diffusion method and the phytochemicals analysed using standard procedures with minor adjustments.
Results and discussion: The study demonstrated thatthe plant contains very important pharmacological compounds. The extracts tested had different inhibitions on the microorganisms tested. Ethyl acetate extract inhibited the growth of all the selected pathogenic organisms while the water extract inhibited the growth of three out of the five microorganisms tested. Hexane and butanol inhibited only Bacillus cereus out of the five microorganisms used in the study. The penicillin which was used as the positive control inhibited the growth of all the organisms, while dimethylsulfoxide(DMSO) did not inhibit the growth of any of the organisms.. One-way analysis of variance (ANOVA) showed there was significant difference in the microorganisms zones of inhibition (P<0.05).
Conclusion: The results obtained in this research are a scientific justification of the plant's use in the treatment of various diseases. The results have revealed thatthe plant has great potency in the treatment against infections caused by all the bacteria tested. However, further research needs to be done to isolate the active compounds, identify their structural formula, their mode of action and their effect in the in vivo environment.
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