OPTIMIZATION OF CULTURE CONDITIONS OF STREPTOMYCES CARPATICUS (MTCC-11062) FOR THE PRODUCTION OF ANTIMICROBIAL COMPOUND
Keywords:
Streptomyces carpaticus (MTCC-11062), Optimization, Antimicrobial compoundAbstract
Objective: To improve the antimicrobial compound productivity of Streptomyces carpaticus (MTCC-11062) by optimizing its physical and chemical parameters
Methods: Streptomyces carpaticus (MTCC-11062) was isolated from Visakhapatnam sea coast of Bay of Bengal and was screened for its antimicrobial activity by using agar well diffusion method. To improve the production of antimicrobial compound the medium composition and physical parameters were optimized and its productivity was studied against Bacillus cereus (MTCC 430) Escherichia coli (MTCC 443), Candida albicans (MTCC 227) obtained from MTCC, Chandigarh, India.
Results: Optimum growth of mycelium and antimicrobial compound production occurred at pH 7.2, agitation 180 rpm and temperature 300C with glucose 10g/L, soyabean meal 2.5g/L, K2HPO4 2g/L, MgSO4 1g/L, NaCl 7.5g/L and trace salts.
Conclusion: The optimization of cultural conditions proposed in this paper has effetely improved the antimicrobial compound productivity of Streptomyces carpaticus (MTCC-11062).
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Blunt JW, Prinsep MR. Marine natural products. J Nat Prod Rep 2006;23:26-78.
Gunasekaran M, Thangavel S. Isolation and screening of actinomycetes from marine sediments for their potential to produce antimicrobials. Int J Life Sci Biotechnol and Pharma Res 2013;2(3):115-26.
Meiying Z, Zhicheng Z. Identification of marine actinomycetes S-216 strain and its biosynthetic conditions of antifungal antibiotic. J Xiamen Univ Nat Sci 1998;37:109-14.
Isoken HO, Ntsikelelo M, Leonard M, Elvis N, Ezekiel G, David AA, Ademola OO, Anthony IO. In vitro time-kill studies of antibacterial agents from putative marine Streptomyces species isolated from the Nahoon beach, South Africa. Afr J Pharm and Pharmacol 2010;4(12):908-16.
Hou Y, Lia F, Wangd S, Qina S, Quan Fu, Wang Q. Intergeneric conjugation in holomycin-producing marine Streptomyces sp. strain M095. J Microbiol Res 2006;163:96-104.
Usha N, Masilamani S. Bioactive compounds produced by Streptomyces strain. Int J Pharm and Pharm Sci 2013;5 (1):176-8.
Sujatha PK, Raju B, Ramana TKV. Studies on a new marine Streptomycetes BT408 producing polyketide antibiotic SBR22 effective against methicillin resistant Staphylococcus aureus. J Microbiol Res 2005;160:119-26.
Selvakumar D. Marine Streptomyces as a novel source of bioactive substances. World J Microbiol and Biotechnol 2010;26 (12):2123-39.
Gopi R, Ramakrishna, Rajagopal. Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites. Egyptian J Biol 2011;13:21-9.
Kumar S, Kannabiran. Diversity and Optimization of process parameters for the growth of Streptomyces VITSVK9 spp. isolated from Bay of Bengal. Ind J Nat Environ Sci 2010;1(2):56-65.
Sambamurthy K, Ellaiah P. A. new streptomycin producing neomycin (B and C) complex Streptomyces marinensis. J Hind Antibio 1974;17:24-8.
Barry AL, Ballows EA, Hawsler W. Thornsberry C. Susceptibility tests:diffusion test procedure. In Manual of Clinical Microbiology, 4thedn eds. J Jr and Shadomy HI;1985. p. 978–87.
Pridham TG, Gottlieb D. The utilization of carbon compounds by some Actinomycetales as an aid for species determination. J Bacteriol 1948;56:170-14.
Brückner R, Titgemeyer F. Carbon catabolite repression in bacteria:choice of the carbon source and autoregulatory limitation of sugar utilization. J FEMS Microbiol Lett 2002;209:141-8.
Postma PW, Lengeler JW, Jacobson GR. Phosphoenolpyruvate: carbohydratephosphotransferase systems of bacteria. J Microbiol Rev 1993;57:543-94.
Dills SS, Apperson A, Schmidt MR, Saier MH Jr. Carbohydrate transport in bacteria. J Microbiol Rev 1980;44:385-418.
Saier MH Jr, Crasnier M. Inducer exclusion and the regulation of sugar transport. J Res Microbiol 1996;147:482-9.
Omura S, Nakagawa A, Yamada H, Hata T, Furusaki A, Watanabe TC. Structure and biochemical properties of Kanamycin A, B, C and D. J Chem Pharm Bull 1973;21:931-34.
Ueki M, Abe K, Hanafi M, Shibata K, Tanaka T, Tanig uchi M. UK-2A, B, C, D. Novel antifungal antibiotics from Streptomyces sp.517–02 I. Fermentation Isolation and Biogical Properties. J Antibiot 1996;49:639–43.
Reichenbach H, Gerth K, Irschikunze HB, Hofle G. Myxobacteria:A source of new antibiotics. J Trends Biotechnol 1988;6:115-21.
Young MD, Kempe LL, Badert FG. Effects of phosphate, glucose, and ammonium on cell growth and lincomycin production by Streptomyces lincolnensis in chemically defined Media. J Biotechnology and Bioengineering 1985;27:327-33.
Williams WK, Edward K. Development of a Chemically Defined Medium for the Synthesis of Actinomycin D by Streptomyces parvulus. J Antimicrob Agents Chemother 1977;11(2):281-90.
Abbas AS, Edwards C. Effects of metals on Streptomyces coelicolor growth and actinorhodin production. J Appl Environ Microbiol 1990;56 (3):675-80.
James PDA, Edwards C, Dawson M. The effects of temperature, pH and growth rate on secondary metabolism in Streptomyces thermoviolaceus grown in a chemostat. J Gen Microbiol 1991;137:1715-1720.
Oskay M. Effects of some environmental conditions on biomass and antimicrobial metabolite production by Streptomyces sp, KGG32. Int J Agric Biol 2011;13:317–24.
Srinivasan MC, Laxman RS, Deshpande MV. Physiology and nutritional aspects of actinomycetes:an overview. World J of Microbiology and Biotechnology 1991;7:171-84.
Sole M, Francia A, Rius N, Lorén JG. The role of pH in the glucose effect†on prodigiosin production by non-proliferating cells of Serratia marcescens. J Letters in Applied Microbiology 1997;25:81-4.
Augustine SK, Bhavsar SP, Kapadnis BP. A non-polyene antifungal antibiotic from Streptomycetes albidoflavus PU 23. J Biosic 2005;30:201-11.