VITRO ANTIOXIDANT PROPERTIES AND WOUND HEALING ACTIVITY OF HYDROETHANOLIC TURMERIC RHIZOME EXTRACT (ZINGIBERACEAE)
Keywords:
Hydroethanolic extract, Turmeric rhizome, Excision wound, Incision wound, Dead space, RatAbstract
Objective: In the present study, we evaluated the ethno pharmacological effect of the different doses of Turmeric rhizome hydroethanolic extract ointment (TRO), using in vitro antioxidant activity and in vivo excision, incision and dead space wound models in Wistar rats.
Methods: The antioxidant activity and acute toxicity studies were performed. In excision wound model, period of epithelization time and wound area were monitored on days 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 using graph paper. Additionally, histological evaluation was carried out on days 3, 7, 14 and 21. Incision wound modle and dead space, were perfomed for breaking strength value and hydroxyproline content.
Results: The results of wound contraction and skin breaking strength a significant increased showed in wound contraction and breaking strength value rate, in all treatment groups with TRO, especially in high dose of TRO. Thus, results of histopathological evaluation showed that TRO accelerate wound healing process specifically in re-epithelization period, angiogenesis and collagen disposition in all treatment groups.
Conclusion: Data revealed that ointment formulation prepared with hydroethanolic extract of Turmeric rhizome had remarkable wound healing activities.
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