ISOLATION AND SCREENING OF ALKALI TOLERANT TRICHODERMA SPP AS BIOCONTROL AGENT FOR ALKALINE AGRICULTURAL SOIL
Keywords:
Trichoderma spp, Alkaline pH, Tolerance, Biomass, BiocontrolAbstract
Objective: The main aim of the study was to screen twenty isolates of Trichoderma spp isolated from high alkaline agricultural soil and non agricultural soil and to test them invitro for their pH levels tolerance and biomass production.
Methods: All the twenty Trichoderma spp isolates were assessed for growth and biomass yield at different alkaline pH 9, 10, 11 on nutrient medium PCA and PCB. The growth were measured in terms of mycelial weight (cell biomass) and sporulation. The most tolerant isolates were further studied for their biocontrol activity against phytopathogen isolated from same location.
Results: Significant variations in the growth of the isolates were recorded with increasing pH. Different isolates of same species also varies in their growth and biomass yield at pH 7, 9, 10, and 11. The isolates were screened for highest growth at pH 11 in terms of biomass yield. 12 isolates showed prominent biomass yield at pH 10.0, but only 5 isolates were able to grow prominently at pH 11
Conclusion: The study concluded that Trichoderma spp isolated from alkali soil with a high pH of more than 11 was only able to grow under high pH range of 9, 10, 11 and also exhibited maximum antagnostic activity for the pathogens studied.
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References
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