ANTIOXIDANT, FREE RADICAL SCAVENGING ACTIVITY AND GC-MS STUDIES ON PEDILANTHUS TITHYMALOIDES (L.) POIT
Keywords:
Pedilanthus tithymaloides, Antioxidant, FTC, TBA, FRAP, ABTS assays, GC-MSAbstract
Objective: To evaluate the methanolic extract of the leaves of Pedilanthus tithymaloides for total phenol, total flavonoid, total antioxidant and free radical scavenging ability and detect the phytoconstituents using GC-MS.
Methods: The total phenols were quantified using Folin-Ciocalteu method. Aluminium chloride method and Phosphomolybdenum method were used to quantify total flavonoid and total antioxidant contentrespectively. In addition to the above, Ferric thiocyanate assay, the thiobarbituric acid assay,Ferric Reducing Antioxidant Power assay and ABTS assay were performed to know the antioxidant potency of the methanolic extract of leaves of Pedilanthus tithymaloides. The phytoconstituents was detected using GC-MS.
Results: The leaves of Pedilanthus tithymaloides recorded a phenolic content of 10.98±0.08 mg TAE/g DW, flavonoid content of 11.49±0.15 µg QE/g DW and total antioxidant content of 6.64±0.05 mg TAE/g DW. The study also revealed significant free radical scavenging ability of the plant leaves as assessed by FTC, TBA, FRAP and ABTS assays. The structural elucidation by GC-MS analysis revealed five different compounds, includingthree esters, an amine and an alkaloid.
Conclusion: The study proves the anticipative potential ability of Pedilanthus tithymaloides, suggesting its exploitation in pharmaceutical applications.
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