ISOLATION AND SCREENING OF MARINE MICROALGAE CHLORELLA SP. _PR1 FOR ANTICANCER ACTIVITY
Keywords:
Chlorella sp _PR1, Anticancer, FACS, IC50 valueAbstract
Objective: The objective of the present study includes isolation, characterization and screening of anticancerous activity against B16F10 cell line using isolated marine microalgae Chlorella sp._PR1.
Methods: In this study, marine microalgae Chlorella sp._PR1 isolated and cultured using f/2 medium and anticancerous activity was assayed using MTT assay.
Results: The DMSO extract of Chlorella sp._PR1 was exhibit anticancerous activity against murine melanoma B16F10 cell line. The extract exhibit reduction of cell viability up to 56% with 2µg/ml concentration. IC50 were calculated and was found that Chlorella sp._PR1 need 5.5 μg/ml of the compounds to reduce the murine melanoma B16F10 cell viability by 50%. Fluorescence activated cell sorting (FACS) analysis revealed that Chlorella sp._PR1 extract (8 µg/ml) brought significant inhibition (p<0.01) of the G0-G1 and the S phase. The extract did not seem to affect the G2-M phase.
Conclusion: DMSO extract of Chlorella sp._PR1 (5.5 µg/ml) was found to be potent against murine melanoma B16F10 cell line.
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References
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