STUDY OF IN VITRO GLUCOSE UPTAKE ACTIVITY OF ISOLATED COMPOUNDS FROM HYDRO ALCOHOLIC LEAF EXTRACT OF CARDIOSPERMUM HALICACABUM LINN
Keywords:
Nil, Cardiospermum halicacabum, Diabetes Mellitus, Glucose uptake Assay, Nil, MTT Assay, L-6 skeletal muscle cellAbstract
Objective: Herbal medicines have long been used effectively in the treatment of Diabetes Mellitus. Cardiospermum halicacabum(C. halicacabum) has been used for several centuries for the treatment of various ailments. The present work was undertaken to study the effect of isolated bioactive compounds from leaf of C. halicacabum on glucose uptake in L-6 cell lines.
Methods: The hydro alcoholic leaf extract of C. halicacabum was subjected to preliminary phytochemical screening and isolated eleven compounds were tested for cytotoxicity by MTT assay. Compound 1 and 8 were selected for further glucose uptake assay based on cytotoxicity concentration CTC50 value. Structure of compound 1 and 8 was identified by NMR technique.
Results: Phytochemical analysis showed the presence of alkaloids, flavonoids, tannins and terpenoids. Compound 1 showed moderate cytotoxic activity to L6 cell line when compared to compound 8. Compounds 1 and 8 were identified as Kaempferol-3-o-α-l-rhamnoside and Apigenin-7-o-β-d-glucuronide. In in vitro glucose uptake assay Kaempferol-3-o-α-l-rhamnoside showed increase in glucose uptake by 11.38 ± 2.04% and Apigenin-7-o-β-d-glucuronide by 15.97 ± 2.16% over control.
Conclusion: Kaempferol-3-o-α-l-rhamnoside and Apigenin-7-o-β-d-glucuronide isolated from C. halicacabum leaf extract enhance the glucose uptake significantly. They exhibit hypoglycemic activity and stimulate glucose uptake in L-6 skeletal muscle cells. This study can bring a promising role for this plant in the management of Diabetes mellitus.
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References
Shoback, David G Gardner, Dolores. In: Greenspan's basic & clinical endocrinology. McGraw-Hill Medical. 9thed. New York; 2011. p. 17.
Maritim AC, Sanders RA, Watkins JB. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol 2003;17(1):24-38.
Ramachandran S, Sri Divya P. Antidiabetic activity of polyherbal extract on alloxan induced diabetic rats. Eur J Biol Sci 2013;5(2):64-7.
Ghanshyam Y, Vipin Kumar G, NishiT, Pragati K. Locomotor activity of methanolic extract ofSaracaindica bark. Adv Bio Res 2013;7(1):01-03.
Grover JK, Yadav S, Vats V. Medicinal plants of India with antidiabetic potential. J Ethanopharmacol 2002;81:81-100.
Mohammed B, Abderrahim Z, Hassane M, Abdelkhaleq L. Medicinal plants with potential antidiabetic activity: a review of ten years of herbal medicine research. Int J Diabetes 2006;14:1-25.
Gopalakrishnan C, Dhananjayan R, Kameswaran L. Studies on the pharmacological actions of Cardiospermumhalicacabum. Indian J Physiol Pharmacol 1976;20:203-6.
Klip A, Ishiki M. Recent development in the regulation of glucose transporter–4 traffic. In: New signals, locations and partners. Endocrinology; 2005. p. 5071-8.
Veeramani C, Pushpavalli G, Pugalendi KV. Antihyperglycaemic effect of Cardiospermumhalicacanum Linn. Leaf extract on STZ induced diabetic rats. J Appl Biomed 2008;6:19-26.
Chisholmm J, Hopkins CY. Fatty acids of the seed oil of Cardiospermum halicacabum. Can J Chem 1985;36:1537-40.
Boonmars T, Khunkitti W, Sirthithaworn P, Fujimake Y. In vitro anti parasitic activity of extract of Cardiospermum halicacabum against third-stage larvae of strongyloidesstercoralis. Parasitol Res 2005;97(5):417-9.
Wakko PJ, Gumede B, Smith P, Folb PI. The in vitro and in vivo antimalarial activity of Cardiospermumhalicacabum L. J Ethanoparmacol 2005;99:137-43.
Khunkitti W, Fujimaki Y, Aoki Y. In vitro anti filarial activity of extract of the medicinal plant Cardiospermum halicacabum against brugiapahangi. J Helminthol 2000;73(3):241-6.
Harborne JB. Phytochemical Methods. In: a guide to modern techniques of plant analysis. Chapman and Hall. 3rd ed. New York; 1998. p. 1-150.
Tomer K, Singh V, Sethiya NK, Singh HP, Kumar M, Chandra D. Isolation and characterization of New Lanosteroid from ethanolic extracts of Eclipta alba Linn. J Pharm Res 2009;2(10):1635-7.
Mueen Ahmed KK. Introduction to isolation, identification and estimation of lead compounds from natural products. In: Textbook of industrial pharmacy. Hiremath SR. Chennai, India, Orient Longman Private Ltd; 2008. p. 345-79.
Hota D. Evalution of plant extracts. In: Bioactive medicinal plants. Gene-Tech Books. New Delhi; 2010. p. 86-7.
Patra JK, Gouda S, Sahoo SK, Thatoi HN. Chromatography separation, 1H NMR analysis and bioautography screening of methanol extract of Excoecaria agallocha L. from Bhitarkanika, Orissa, India. Asian Pac J Trop Biomed 2012;50-6.
Ly TN, Shimoyamada M, Kato K, Yamauchi R. Isolation and characterization of some antioxidative compounds from the rhizomes of smaller Galangal (Alpinia officinarum Hance). J Agric Food Chem 2003;51:4924-9.
Francis D, Rita L. Rapid colorometric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. JIM 1986;89:271-7.
Gupta RN, Pareek A, Rathore GS, Suthar M. Study of glucose uptake activity of Helicteres isora Linn. fruits in L-6 cell lines. Int J Diabetes Dev Ctries 2009;29:170-3.
Trinder P. Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J Clin Pathol 1969;22(2):158-61.
Baron AD, Brechtel G, Wallace P, Edelman SV. In: Skeletal muscle. Am J Physiol 1988. p. 769–74.
Shulman GI. Cellular response of insulin resistance. J Clin Invest 2000;106(2):171–6.
Sophie E, Jeremy M. The molecular basis of insulin-stimulated glucose uptake: signaling, trafficking and potential drug targets. J Endocrinol 2009;203:1–18.
Sudharshan Reddy D, Srinivasa Rao A, Pradeep Hulikere A. In vitro antioxidant and glucose uptake effect of Trichodesmaindicum in L-6 cell lines. Int J Pharm Bio Sci 2012;3(4):810–9.
Yarasu N, Smana P, Pavankumar R, Nareshchandra RNBS, Vinil Kumar V. In vitro glucose uptake assay of hydro methanolic leaves extract of Syzygium jambos (L) alston in rat skeletal muscle (L6) cell lines. Indo Am J Pharm Res 2013. p. 7336-41.
Anitha Mary M, Sujith K, Santosh P, Christina AJM. Study of glucose uptake activity of solanum xantohocarpum in L-6 Cell Lines. Euro J Biol Sci 2013;5(3):77-81.
Premkumar N, Soumya K, Dharsana JN, Seena K, Anjana AK. Comparing the effect of antidiabetic activity of Andrographis paniculata, Salacia reticulate and Ocimum sanctum by in vitro screening. Asian J Pharm Clin Res 2012;5(4):146-9.
Gupta RN, Pareek A, Rathore GS, Suthar M. Study of glucose uptake activity of Helicteresisora Linn. fruits in L-6 cell lines. Int J Diabetes Dev Ctries 2009;29:170-3.
Patel MB, Mishra SH. Cell lines in diabetes. Phcog Res 2008;2:188-205.
Defronzo RA, Jacot E, Jequier E, Maeder E, Wahren J, Felber JP. The effect of insulin on the disposal of intravenous glucose: results from indirect calorimetry and hepatic and femoral venous characterization. Diabetes 1981;30:1000-7.