ANTIBACTERIAL ACTIVITY OF SELECTED MEDICINAL PLANT IN TRADITIONAL TREATMENT OF WOUND INFECTION IN SOUTHEAST INDIA
Keywords:
Pithecellobium dulce, Wound healing, Antibacterial activity, Herbal extractsAbstract
Objective: The wound healing potential of fruit peel of Pithecellobium dulce Benth, a plant traditionally used for wound treatment against wound- infecting bacteria, was assessed using disc diffusion and microtitre plate methods.
Methods: P. dulce fruit peel was extracted using ethyl acetate, methanol and water and tested for their antimicrobial activities against bacterial isolates from wound infection using disc diffusion and microtitre plate methods.
Results: All the three extracts had antimicrobial activities, among which methanol extract had higher activities against all the tested organisms compared to the ethyl acetate and aqueous extracts. The most susceptible organism to all the extracts was K. pneumonia and S. aureus while the most resistant were P. mirabilis and P. vulgaris.
Conclusion: The potential antibacterial activity of P. dulce plant against both gram-positive and gram-negative bacteria justifies the use of the plant as the wound healing agent. The isolation of active principle of this plant may serve as source and lead for the synthesis of drugs in pharmaceutical industries.
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References
Sharma S, Singh T, Vijayvergia R. Molluscidal activity of some medicinal plants. J Herb Med Toxicol 2009;3(2):155-7.
Martin P. Wound healing–Aiming for perfect skin regeneration. Sci 1991;276(5309):75-81.
Shenoy C, Patil MB, Kumar R, Patil S. Preliminary phytochemical investigation and wound healing activity of Allium cepa Linn. (Liliaceae). Int J Pharm Pharm Sci 2009;2(2):167-75.
Saikat D, Sekhar KB, Ranabir S, Subhash CM. Antidiabetic affect of matured fruits of Diospyros peregrine in Alloxan-induced diabetic rats. Int J Green Pharm 2008;2(2):902-29.
Menghani E, Jain SC, Jain R. Scientific validation of Piper nigrum by HPLC and anti-oxidative assay markers. Asian J Biotechnol 2010;2:133-8.
Anonymous. The Wealth of India: Raw materials, Council of Scientific and Industrial Research, New Delhi; 1969;3:140.
Norrell SA, Messley KE. Microbiology laboratory manual. Principles and applications. Prentce-Hall, Inc. New Jersey; 1997.
Cheesbrough M. Morphology and characterization of E. coli and S. aureus. District Laboratory practice in tropical countries part II. Cambridge University; 2004. p. 157-79.
Sukantha TA, Shubashini K Sripathi, Ravindran NT, Balashanmugam P. Antioxidant and antibacterial activities of Trianthema decandra Linn. Int J Pharm Pharm Sci 2012;4(1):410-3.
Jorgensen JH, Turnidege JD, Washington JA. Antibacterial susceptibility tests: Dilution and disk diffusion method. In: Murray PR, Pfaller MA, Tenover FC, Baron EJ, Yolken RH, ed. Manual of clinical microbiology, 7th ed. Washington, DC: ASM Press; 1999. p. 1526-43.
Govindarajan M, Jebanesan A, Reetha D, Amsath R, Pusphanathan T, Samidurai K. Antibacterial activity of Acalypha indica L. Eur Rev Med Pharm Sci 2008;12;299-302.
Isibor JO, Oseni A, Eyaufe A, Ahmadu T. Incidence of aerobic bacteria and Candida albicans in post-operative wound infections. Afr J Microbiol Res 2008;2:288-91.
Anupurba S, Bhattacharjee A, Garg A, Sen MR. Antimicrobial susceptibility of Pseudomonas aeruginosa isolated from wound infections. Ind J Dermatol 2006;51:286-8.
Howell-Jones RS, Wilson MJ, Hill KE, Howard AJ, Price PE, Thomas DW. A review of the microbiology, antibiotic usage and resistance in chronic skin wounds. J Antimicrobial Chemother 2005;55:143-9.
Dhar S, Saraf R, Singh K, Raina B. Microbiological profile of chronic burn wounds among patients admitted in burn unit. J Med Educ Res 2007;9:182-5.
Clark RAF. Fibrin and wound healing: Ann New York; Acad Sci 2001;936(1):355–67.
Mensah AY, Sampson J, Houghton PJ. Effects of Buddleja globosa leaf and its constituents relevant to wound healing. J Ethnopharmacol 2001;7(2-3):219–26.
Marjorie MC. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12(4):564-82.
Hagermant A, Butler LG. The specificity of proanthocyanidin-protein interactions. J Biol Chem 1981;256:4494-7.
Suhaila N. Darogha. Antibacterial activity of Quercus infectoria extracts against bacteria isolated from wound infection. J Kirkuk Univ Sci Studies 2009;4(1):20-30.
Karodi R, Jadhav M, Rub R, Bafna A. Evaluation of the wound healing activity of a crude extract of Rubia cordifolia L. (Indian madder) in mice. Int J Appl Res Nat Prod 2009;2(2):12-8.
Davis R. Inhibitory and stimulatory systems in Aloe vera. Aloe Today Winter; 1991-1992.
Sharma UK, Singh A, Sharma U, Kumar M, Rai D, Agrahari A. Wound healing activity of Kigelia pinnata bark extract. Asian J Pharm Clin Res 2010;3(4):73-5.
Malik JK, Manvi FV, Nanjware BR, Singh S. Wound healing properties of alcoholic extract of Gymnema sylvestre R. BR leaves in rats, J Pharm Res 2009;2(6):1029-30.
Abubakar Amali Muhammad, Nur Aimi Syarina Pauzi, Palanisamy Arulselvan, Faridah Abas, Sharida Fakurazi. In Vitro Wound Healing Potential and Identification of Bioactive Compounds from Moringa oleifera Lam; 2013.