ANTIBACTERIAL ACTIVITY OF FRESH WATER CRAB AND SNAIL AND ISOLATION OF ANTIBACTERIAL PEPTIDES FROM HAEMOLYMPH BY SDS – PAGE
Keywords:
Antimicrobial, Innate immunity, Haemolymph, TLC, SDS PAGE, Snail, CrabAbstract
Objective: The present study was undertaken to characterize antimicrobial molecules from the fresh water snail and crab.
Methods: Collection of haemolymph, preparation of extracts, antimicrobial activity, TLC analysis, SDS PAGE analysis.
Results: The result of the present investigation reported that the fresh water snail (Pomacea insularium) and crab (Callinectes sapidus) having remarkable antimicrobial activity in methanol, di-ethyl ether and water extracts. Antimicrobial activity was high in di-ethyl ether extracts of the snail against Streptococcus sp. (37.16±0.76 mm) and methanol extract of crab against E. coli (32.16±0.28 mm). The MIC of extracts ranges between 5 µl to 30 µl methanol extract of both snail and crab inhibited the growth of organisms at very low concentration. Biomolecules from the extract was separated by TLC. The molecular mass of the peptide was determined by SDS PAGE. Peptides from snail and crab haemolymph were ranges in 9 to 110 and 40 to 100 kDa respectively.
Conclusion: The present findings suggest that fresh water crab and snail having good antimicrobial activity against pathogenic microbes. Therefore they can be used to treat many pathogenic infections.
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