α-GLUCOSIDASE AND α-AMYLASE INHIBITORY ACTIVITY OF INDIGOFERA CORDIFOLIA SEEDS AND LEAVES EXTRACT
Keywords:
Indigofera cordifolia, Nil, Nil, Diabetes mellitusAbstract
Objective: The present study was done to find out the anti-diabetic effect of Indigofera cordifolia seeds and leaves extract on intestinal α–glucosidase, α-amylase enzymes In-vitro.
Methods: In-vitro α–glucosidase and α-amylase assays were performed in order to evaluate anti- diabetic potential of Indigofera cordifolia seeds and leaves extract. The dose depended inhibitory effect of aqueous and methanol extract of seeds and leaves was compared with standard acarbose.
Results: α-Amylase inhibitory activity of aqueous extract of seeds and leaves was found to be 65.12% and 55.02% while methanol extract showed 85.59% and 83.40 % inhibition at 4 mg/ml, respectively. In α–glucosidase inhibitory assay the methanol extract of seeds and leaves show more maltase inhibition (IC50 = 2.57±0.29 and 3.47±0.87 mg/mL, respectively) than sucrase (IC = 50 2.73±0.11 and 3.71± 0.46 mg/mL, respectively), at the same time aqueous extract of seeds and leaves showed more maltase inhibition (IC50 = 3.30±0.94 and 5.97± 0.22 mg/mL, respectively) than sucrase inhibition (IC50 = 4.30±0.16 and 6.56±0.62 mg/mL, respectively). Acarbose (standard) showed more maltase inhibition (IC50 = 9.86±0.12 μg/mL) than sucrase (IC50= 46.46±1.5 μg/mL) and had 93.40% α-amylase inhibition at 50 μg/ml.
Conclusion: Both the methanol and aqueous extract of I. cordifolia seeds and leaves showed strong inhibition against animal α-amylase and α-glucosidase enzyme.
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