KINETICS AND MODELING OF TANNASE PRODUCTION USING ASPERGILLUS FOETIDUS IN BATCH FERMENTATION
Keywords:
Aspergillus foetidus, Tannase, Kinetics and Modeling, Redgram huskAbstract
Objective: To produce tannase enzyme using Aspergillus foetidus with red gram husk as substrate in batch fermentor and investigate the suitable un structured kinetic model for the system.
Methods: The present study was done via two steps. At first to study the maximum production of tannase enzyme by Aspergillus foetidus (MTCC 3557) using red gram husk as a substrate in a modular ferment or followed by to develop the kinetic model of tannase production.
Results: The maximum tannase activity and biomass concentration were found to be 157.26 U/ml and 7.12 g/l respectively at the end of 96 hours of fermentation. The biomass yield coefficient (YX/S) and the product yield coefficient (YP/S) were found to be 0.23 g of biomass/g of substrate and 21.2 U/g of substrate respectively. Logistic model, Luedeking-Piret model and substrate utilization kinetic model were found to represent closely the experimental data of growth kinetics, product formation kinetics and substrate utilization kinetics respectively.
Conclusion: Tannase enzyme production was studied using A. foetidus with redgram husk as substrate by modular fermenter and suitable models were predicted. The kinetic parameters were also estimated by fitting the data to the model using the Lineweaver-Burk method.
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References
Lekha PK, Lonsane BK. Production and application of tannin acyl hydrolase; state of the art. Adv Appl Microbiol 1997;44:215–60.
Aguilar CN, RodrÃguez R, Gutiérrez-Sánchez, Augur C, Favela-Torres E, Prado-Barragan LA, et al. Microbial tannases: advances and perspectives. Appl Microbiol Biotechnol 2007;76:47–59.
Deschamps AM, Otuk G, Lebeault JM. Production of tannase and degradation of chestnut tannins by bacteria. J Ferment Technol 1983;61:55-9.
Aoki K, Shinke R, Nishira H. Purification and some properties of yeast tannase. Agr Biol Chem 1976;40(1):79-85.
Bajpai B, Patil S. Tannin acyl hydrolase (EC 3.1.1.20) activity of Aspergillus, Penicilium, Fusarium and Trichoderma. World J Microbiol Biotechnol 1996;12:217-20.
Bradoo S, Gupta R, Saxena RK. Screening of extracellular tannase producing fungi: development of a rapid and simple plate assay. J Gen Appl Microbiol 1996;42:325-9.
Bhat TK, Singh B, Sharma OP. Microbial degradation of tannins–a current perspective. Biodegradation 1998;9:343-57.
Chae SK, Yu TJ. Experimental manufacture of acron wine by fungal tannase. Hanguk Sipkum Kwahakhoechi 1983;15:326–32.
Mohan SK, Viruthagiri T, Arunkumar C. Statistical Optimization of Process parameters for the production of tannase by Aspergillus flavus under submerged fermentation. 3 Biotech 2014;4(2):159–66.
Coggon P, Sanderson GW. Manufacture of instant tea. Patent Ger Offen 1973;110:54-8.
Mohan Kuppusamy, Viruthagiri Thangavelu, Arunkumar Chockalingam. Optimization of submerged fermentative production of Tannase by Aspergillus flavus. Int J Chem Tech Res 2012;4(4):1461-7.
Couto SR, Sanroman MA. Application of solid-state fermentation to food industry-a review. J Food Engg 2006;76(3):291–302.
Lin Y, Tanaka S. Ethanol fermentation from bio mass resources: current state and prospectus. Appl Microbiol Biotechnol 2006;69:627-42.
Mondal KC, Banerjee D, Jana M, Pati BR. Calorimetric assay method for determination of the tannase activity. Anal Biochem 2001;295:168-71.
Narasimha G, Sridevi A, Viswanath B, Subhosh Chandra M, Rajasekar reddy B. Nutrient effects on production of cellulytic enzymes by A. niger. Afr J Biotechnol 2005;5(5):472-6.
Haggerman AE, Butler LG. Protein precipitation method for determination of tannins. J Agric Food Chem 1978;26:809–12.
James Bailey, David Ollis. Biochemical Engineering Fundamentals, 2nd ed; 2010.
Luedeking R, Piret EL. A Kinetic study of the lactic acid fermentation. J Biochem Microbiol Technol Eng 1959;67(6):1393-412.
Weiss RM, Ollis DF. Extracellular microbial polysaccharides: Substrate, biomass and Product kinetic equations for batch xanthan gum fermentation. Biotechnol Bioengg 1966;22:859-73.