POTENTIAL HEALING EFFECTS OF HIBISCUS SABDARIFFA L. FLOWERS ON ARTHRITIS
Keywords:
Hibiscus sabdariffa L, Nil, Erythrocyte sedimentation rate (ESR), C-reactive protein level (CPR) andthe serumm copper levelAbstract
Objective: The present study has been focused to assess the anti-inflammatory activity and healing effects of the aqueous extract of Hibiscus sabdariffa L. Flowers on induced arthritis in mice and compare it with meloxicam (Mobic®), one of the most conventional drugs used to treat arthritis.
Methods: The water extract of Hibiscus sabdariffa was administered orally at a dose of 300 mg/kg, 400 mg/kg and 500 mg/kg body weight for 14 days after induction of arthritis with incomplete fruend's adjuvant.
Results: (T1, T2 and T3) showed a significant increase in body weight when compared with T4, negative and positive control groups. A significant decrease in the levels of RBC and Hb was observed in all groups subjected to arthritic (T1,T2,T3,T4 and T5) when compared to the negative group (T6). The administration of the aqueous extract of Hibiscus sabdariffa L. flowers to arthritic mice in (T1, T2 and T3) improved the levels of Hb and RBC to near normal. A significant reduction (P≤0.01) in spleen weight,WBC, ESR, CPR and serum copper level was found at all treatment groups with the water extract of Hibiscus sabdariffa in comparison with the groups treated with meloxicam, the positive and the negative.(T1, T2 and T3) reveled a significant (P≤0.01) reduction of the inflammation in comparison with the other treatment groups (T4, T5). A better activity was observed at 500mg/kg body weight in mice.
Conclusion: The water extracts of Hibiscus sabdariffa L. flowers reveled a significant anti-inflammatory activity in albino mice at a dose of 300, 400 and 500 mg/kg body weight. Whereas 500 mg/kg in mice proved to possess better healing effects
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References
Heinrich M, Gibbons S. Ethnopharmacology in drug discovery: an analysis of its role and potential contribution. J Pharm Pharmacol 2001;53:425–32.
Tori Hudson. ND hibiscus hawthorn and the heart. Nat Med J 2011;3:7.
Onyenekwe PC, Ajani EO, Ameh DA, Gamaniel KS. Antihypertensive effect of roselle (Hibiscus sabdariffa) calyx infusion in spontaneously hypertensive rats and a comparison of its toxicity with that in wistar rats. Cell Biochem Funct 1999;17(3):199-206.
H Mozaffari-Khosravi, B-A Jalali-Khanabadi, M Afkhami-Ardekani, F Fatehiand M Noori-Shadkam. The effects of sour tea (Hibiscus sabdariffa) on hypertension in patients with type II diabetes. J Hum Hypertens 2009;23:48–54.
Herrera-Arellano A, Miranda-Sanchez J, Avila-Castro P. Clinical effects produced by a standardized herbal medicinal product of Hibiscus sabdariffa on patients with hypertension. A randomized, double-blind, Lisinopril-controlled clinical trial. Planta Med 2007;73:6-12.
Odigie IP, Ettarh RR, Adigun SA. Chronic administration of aqueous extract of Hibiscus sabdariffa attenuates hypertension and reverses cardiac hypertrophy in 2K-1C hypertensive rats. J Ethnopharmacol 2003;86(2-3):181-5.
Herrera-Arellano S, Flores-Romero MA. Chavez-Soto Effectiveness and tolerability of a standardized extract from Hibiscus sabdariffa in patients with mild to moderate hypertension: a controlled and randomized clinical trial. J Tortoriello Phytomed 2004;11:374–82.
Kim MS, Kim JK, Kim HJ, Moon SR. Hibiscus extract inhibits the lipid droplet accumulation and adipogenic transcription factors expression of 3T3-L1 preadipocytes. J Altern Complement Med 2003;9(4):499-504.
Gurrola-Diaz C, Garcia-Lopez P, Sanchez-Enriquez S. Effects of Hibiscus sabdariffa extract powder and preventive treatment (diet) on the lipid profiles of patients with metabolic syndrome (MeSy). Phytomed 2010;17:500-5.
Kuriyan R, Kumar D, Rajendran R, Kurpad A. An evaluation of the hypolipidemic effect of an extract of Hibiscus sabdariffa leaves in hyperlipdemic Indians: a double blind, placebo controlled trial. BMC Compl Alt Med 2010;10:27.
Chen CC, Hsu JD, Wang SF. Hibiscus sabdariffa extract inhibits the development of atherosclerosis in cholesterol-fed rabbits. J Agric Food Chem 2003;27:51(18):5472-7.
Suboh SM, Bilto YY, Aburjai TA. Protective effects of selected medicinal plants against protein degradation, lipid peroxidation and deformability loss of oxidatively stressed human erythrocytes. Phytother Res 2004;18(4):280-4.
Tseng TH, Hsu JD, Lo MH. Inhibitory effect of Hibiscus protocatechuic aid on tumorpromotion in mouse skin. Cancer Lett 1998;126(2):199-207.
Tseng TH, Kao TW, Chu CY. Induction of apoptosis by hibiscus protocatechuic acid in human leukemia cells via reduction of retinoblastoma (RB) phosphorylation and Bcl-2 expression. Biochem Pharmacol 2000;60(3):307-15.
Najla Gooda Sahib, Nazamid Saari, Amin Ismail, Alfi Khatib, Fawzi Mahomoodally, Azizah Abdul Hamid. Plants' metabolites as potential antiobesity agents. Sci World J Published; 2012.
Porter BB, Park N, Richardson C, Vainio K. Rheumatoid arthritis of the result of synovectomy. J Bone Joint Surg 1974;56:427-37.
Hafstrom I, Ringertz B, Spangberg A. Avegan diet free gluten improves the signs and symptoms of rheumatoid arthritis. Rheumatol 2001;40(10):1175-9.
Grigor C, Capell H, Stirling A. Effect of a treatment strategy of tight control for rheumatoid arthritis (the TICORA study): a single-blind randomised controlled trial. Lancet 2004;364:263–9.
Sumaia Awad Elkariem Ali, Abdelwahab Hassan Mohamed, Galal Eldin Elazhari Mohammed. Fatty acid composition, anti-inflammatory and analgesic activities of Hibiscus sabdariffa Linn. Seed J Adv Vet Anim Res 2014;1(2):50-7.
Marri Praveen, M Janarthan. Evaluation of anti arthritic activity of aqueous extract of Hibiscus Platinifolius in albino rats. IJRPB 2013;1(6):815-8.
Rasool M, Marylatha L, Varalakshmi P. Effect of Withania somnifera on lysosomal acid hydrolases in adjuvant-induced arthritis in rats. Pharm Pharmacol Commun 2000;6:187–90.
Glen EM, Bowman BJ, Ronloff NA, Seely RJ. A major contributory cause of arthritis in adjuvant inoculated rats. Granulocytes. Agents Actions 1977;7:265-82.
Citation H Rehman. hemolytic anemia following mycoplasma infection. Int J Hematol 2008;4:1.
Allar S, O'Driscoll J, Laurie A. Salmonella osteomyelitis in aplastic anaemia after anti-lymphocytic globulin and steroid treatment. J Clin Pathol 1977;2:174-5.
Mowat G. Haematological abnormalities in rheumatoid arthritis. Semin Arthritis Rheum 1971;1:195-9.
Joe B, Wilder RL. Animal models of rheumataiod arthritis. Mol Med 1999;5:367-9.
Carlson BC, Jansson AM, Larsson A, Bucht A, Lorentzen JC. The endogenous adjuvant squalene can induce a chronic T-cell mediatyed arthritis in rats. Am J Pathol 2000;156:2057-65.
Soodabeh Saeidnia, Azadeh Manayi, Ahmad Reza Gohari, Mohammad Abdollahi. The story of beta-sitosterol-a review. Eur J Med Plant 2014;4(5):590-609.
Grant NH, Alburn HE, Kryzanauskas C. Stabilization of serum albumin by anti-inflammatory drugs. Biochem Pharmacol 1970;19:715-22.
McConkey B, Crockson RA, Crockson AP, Nilkinson AR. The effect of some anti inflammatory drugs on the acute-phase proteins in rheumatoid arthritis. Q J Med 1973;32:785-91.
Thompson D, Pepys MB, Wood SP. "The physiological structure of human C-reactive protein and its complex with phosphocholine". Structure 1999;7(2):169–77.
Revnic F. The Significance of serum ceruloplasmin in diagnosis of rheumatoid arthritis. Toxicol Lett 1995;(78 Suppl 1):70-70.
White AG, Scudder P, Dormandy TL, Martin VM. Copper-an index of erosive activity? Rheumatol 1978;17:3-5.
Abdel-Monem Ateya, Zeinab I, El Sayed, Mona Fekry. Chemical Constituents, Cytotoxicity, Anti-oxidant, Hypoglycemic and antihypertensive activities of egyptian hibiscus trionum. Aust J Basic Appl Sci 2012;6(3):756-66.
Marcela B Oliveira, Daniela Cm Vieira, Thalita M Fza, Hrida Rn Salgado, Marlus Chorilli. Microbiological control of moisturizing mask formulation added of hibiscusflowers, assai palm, black mulberry and papaw glycolic extracts. Int J Pharm Pharm Sci 2013;5(1):342-5.
Sawatzky D, Willoughby D, Colville-Nash P, Rossi A. The involvement of the apoptosis-modulating proteins Erk 1/2, Bcl-xL, and Bax in the resolution of acute inflammation in vivo. Am J Pathol 2006;168:33-41.
Sridhar C, Krishnaraju AV, Subbaraju GV. Antiinflammatory constituents of teramnus labialis. Indian J Pharm Sci 2006;68:111-4.