SPECTROPHOTOMETRIC DETERMINATION OF ARIPIPRAZOLE, CLOZAPINE AND SULPIRIDE BY ION- PAIR EXTRACTIONIN IN TABLETS AND BIOLOGICAL FLUIDS
Keywords:
Aripiprazole, Clozapine, Sulpiride, Ion-pair complex, Bromophenol blue, Bromothymol blueAbstract
Objective: Simple, sensitive and extraction-free spectrophotometric methods have been developed for the determination of three antipsychotics drugs, namely aripiprazole (ARP), clozapine (CLP) and sulpiride (SUP) both in tablets and in biological fluids.
Methods: Two spectrophotometric methods are based on the formation of yellow colored ion-pair complexes between the studied drugs and two sulphonphthalein acid dyes, bromophenol blue (BPB) and bromothymol blue (BTB) with absorption maxima at 408 and 406 nm, respectively.
Results: Molar ratio of formed ion-pair complex was found to be 1:1 as deduced by Jobʼs method. Several parameters such as pH and buffer type, reagent volume, sequence of addition and effect of extracting solvent were optimized to achieve high sensitivity, stability, low blank reading and reproducible results. Various analytical parameters have been evaluated and the results have been validated by statistical data. A proposal for the reaction pathway was postulated.
Conclusion: The proposed methods were successfully applied to the analysis of commercial tablets containing the drugs and the results were in good agreement with those obtained with reported methods. The proposed methods were further applied to the determination of the studied drugs in spiked human serum and urine.
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