• Monika P. Department of Biotechnology M. S. Ramaiah Institute of Technology, MSRIT Post, Bengaluru-560054, India
  • Basava Raj B. V. Department of Pharmaceutics M. S. Ramaiah College of Pharmacy, MSRIT Post, Bengaluru 560054, India
  • CHIDAMBARA MURTHY K. N. Division of Research and Patents Central Research Laboratory, M. S. Ramaiah Medical College and Teaching Hospital, MSRIT Post, Bengaluru 560054, India
  • Ahalya N. Department of Biotechnology M. S. Ramaiah Institute of Technology, MSRIT Post, Bengaluru-560054, India
  • Kruthi Gurudev Department of Pharmaceutics M. S. Ramaiah College of Pharmacy, MSRIT Post, Bengaluru 560054, India


Catechin, Flavanoid, Bioavailability, Eudragit L 100, Sustained release


Objective: The aim of the study was to develop sustained release nanocapsules of catechin rich extract (CRE) using Eudragit L 100 as a polymer by emulsion solvent evaporation technique with a major focus on enhancing its bioavailability.

Methods: CRE and the polymer were subjected for physical compatibility studies using Fourier transform infrared spectroscopy (FT-IR). Nanoparticles were evaluated for percentage yield, drug (catechin) entrapment efficiency and further characterized by scanning electron microscopy (SEM),X-Ray diffraction (XRD) and differential scanning calorimetric (DSC) studies. The in vitro release study was carried out for 12 hours at pH 6.8 and pH 7.4 and the results obtained were fit to kinetic models. Concentration of residual methanol was determined by Gas chromatography (GC).

Results: All prepared particles were spherical, non-porous and were in nano-meter range (50-160 nm). The XRD and DSC results suggest that CRE existed in amorphous state in the nanoformulations. The results of in vitro release study for nanocapsules showed the highest drug release of 97.27 % at pH 6.8 (NF1 nanoformulation-1) and low of 67.60 % was observed for (NF3 nanoformulation-2)atpH 7.4. The in vitro release data of nanoformulations showed the highest correlation for Higuchi matrix and Korsmeyer-Peppas, which indicated that drug release occurred via fickian diffusion mechanism. Concentration of methanol, which was used as solvent for the formulation of nanoparticles, was well within the permitted levels, suggesting the safety for oral application.

Conclusion: Based on the results, it may be worth to consider further studies on catechinnanoformulations for pilot studies, in vivo studies for clinical application.


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Jin Ze Xu, Sai Ying Venus Yeung, Qi Chang, Yu Huang, Zhen-Yu Chen. Comparison of antioxidant activity and bioavailability of tea catechins with their epimers. Br J Nutr 2004;91:873–81.

Chidambara Murthy KN, Kim J, Vikram A, Patil BS. Differential inhibition of human colon cancer cells by structurally similar flavonoids of citrus. Food Chem 2012;132(1):27-34.

Agrawal AD. Pharmacological activities of Flavanoids: A Review. Int J Pharm Sci Nanotechnol 2011;4(2):1394-8.

Vanna SannaI mtiaz, Siddiqui A, Mario Sechi, Hasan Mukhtar. Nanoformulation of natural products for prevention and therapy of prostate cancer. Cancer Lett 2013;334:142–51.

Gupta R, Ramteke PW. Antimicrobial activity of Emblicaofficinalis, Saracaindica and Terminalia arjuna against Multi-Drug Resistant (MDR) Bacterial Pathogens. Hacettepe J Biol Chem 2011;39(4):435–7.

Schroeter H, Heiss C, Balzer J, Kleinbongard P, Keen CL, Hollenberg NK, et al. (−)-Epicatechin mediates beneficial effects of flavanol-rich cocoa on vascular function in humans. Proc Natl Acad Sci USA 2006;103:1024–9.

Fraga Cesar G, Oteiza Patricia I. Dietary flavanoids: Role of (-)-epicatechin and related procyanidins in cell signalling. J Free Rad Biol Med 2011;51:813-23.

Zhu Min, Chen Yu, Ronald C Li. Oral absorption and bioavailability of tea catechin. Plant Med 2000;66:444-7.

Kohri T, Nanjo F, Suzuki M. Synthesis of (_)-[4-3H] epi-gallocatechingallate and its metabolic fate in rats after intravenous administration. J Agric Food Chem 2001;49:1042–8.

Tasnima Khushnud, Shaker A, Mousa. Potential Role of Naturally Derived Polyphenols and Their Nanotechnology Delivery in Cancer. Mol Biotechnol 2013;55:78–86.

McNeil SE. Nanotechnology for the biologist. J Leukocyte Biol 2005;78:585-94.

AdlinJino Nesalin J, Anton Smith A. Preparation and evaluation of chitosan nanoparticles containing zidovudin. Asian J Pharm Sci 2012;7(1):80-4.

Jai Prakash B, Gaja Sayyad FJ. Preparation and Evaluation of Nanodispersions of Telmisartan by various techniques. Inventi Impact NDD 2013;2:151-9.

Hu Daode, Liu Liang, Chen Wenjuan, Li Sining, Zhao Yaping. A Novel Preparation Method for 5-Aminosalicylic Acid Loaded Eudragit S100 Nanoparticles Int J Mol Sci 2012;13:6454-68.

Zu Y, Wang D, Zhao X, Jiang R, Zhang Q, Zhao D, et al. A novel preparation method for camptothecin (CPT) loaded folic acid conjugated dextran tumor-targeted nanoparticles. Int J Mol Sci 2011;12:4237–49.

Bharathi M, Sarat Chandra Prasad M, Latha Eswari R, Wasim Raja S, Ravi Teja Allena, Brito Raj S, et al. Preparation and in vitro & in vivo characterization of valsartan loaded eudragit nanoparticles. Der Pharm Sin 2012;3(5):516-25.

Sudheer Nadendh, Snehalatha, Nagaraja TS, Bharathi. Effect of various polymers on swelling and in vitro release of rampril in effervescent system. Int J Pharm Life Sci (IJPLS) 2013;4(4):2621-5.

Kalam MA, Humayun M, Parvez N, Yadav S, Garga. Release kinetics of modified pharmaceutical dosage forms: a review. Cont J Pharm Sci 2007;1:30-5.

Sharma A, Shukla T, Indira M. Design, Development and Evaluation of AceclofenacSustained release matrix tablet. Int J Drug Res 2011;3(1):307–12.

Bharate SS, Bharate SB, Bajaj AN. Interactions and incompatibilities of pharmaceutical excipients with active pharmaceutical ingredients: A comprehensive review. J Excip Food Chem 2010;1:3–26.



How to Cite

P., M., B. R. B. V., C. M. K. N., A. N., and K. Gurudev. “DEVELOPMENT OF SUSTAINED RELEASE NANOCAPSULES OF CATECHIN RICH EXTRACT FOR ENHANCED BIOAVAILABILITY”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 11, Nov. 2014, pp. 331-7,



Original Article(s)