ISOLATION AND CHARACTERIZATION OF STIGMASTEROL FROM CHLOROFORM FRACTION OF AERIAL PART OF ARGEMONE MEXICANA L
Keywords:
Phytosterols, Argemone mexicana, Stigmasterol, Isolation, PapaveraceaeAbstract
Objective: Phytosterols are group of steroidal alcohol play important roles in structural component in the cell membrane and play a role in membrane stability. There are almost 22 different sterols are found yet and the major phytosterols include ß-sitosterol, campesterol and stigmasterol. The objective of this study was to isolate and characterize the bioactive principles from the chloroform fraction of Argemone Mexicana L.
Methods: The isolation was done using column chromatography using gradient elution with different mobile phases. The isolated compound was subjected to spectral analysis. Structure elucidation was carried out on basis of spectral analysis.
Results: The chemical investigation of the chloroform fraction of aerial parts of belonging to the family Papaveraceae led to the isolation of stigmasterol. The isolated compounds were characterized using various spectroscopic data as well as chemical studies.
Conclusion: From the spectral characteristics, the isolated compound from the chloroform fraction of aerial parts was confirmed to be stigmasterol. This is the first ever report of these stigmasterol compound from the chloroform fraction of aerial parts of Argemone mexicana.
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References
Chang HM, But PH. Pharmacology and applications of Chinese materia medica. Vol. 1-2. Singapore: World Scientific Publishing; 1986.
Kapoor LD. CRC Handbook of ayurvedic medicinal plants. Boca Raton, Florida: CRC Press; 1990. p. 416.
Farnsworth NR, Akerele O, Bingel AS, Soejarto DD, Guo Z. Medicinal plants in therapy. Bull W H O 1985;63:965-81.
Ibrahim HA, Ibrahim H. Phytochemical screening and toxicity evaluation on the leaves of Argemone mexicana Linn. (Papaveraceae). Int J Appl Sci 2009;3:39-43.
Bhalke RD, Gosavi SA. Anti-stress and antiallergic effect of Argemone mexicana stems in asthma. Arch Pharm Sci Res 2009;1:127–9.
Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants. New Delhi: NISCOM, CSIR; 1956. p. 23.
Ambasta SP. The useful plants of India New Delhi: PID, CSIR; 1986. p. 51.
Sharma J, Gairola S, Gaur RD, Painuli RM. The treatment of jaundice with medicinal plants in indigenous communities of the Sub-Himalayan region of Uttarakhand, India. J Ethnopharmacol 2012;143:262-91.
Bose BC, Vijayvargiya R, Saifi AQ, Sharma SK. Chemical and pharmacological studies on Argemone mexicana. J Pharm Sci 1963;52:1172-5.
Prajapati ND, Purohit SS, Sharma AK, Kumar T. A handbook of medicinal plants. Jodhpur, India: Agrobios; 2003. p. 59-60.
Savithramma N, Sulochana CH, Rao KN. Ethnobotanical survey of plants used to treat asthma in Andhra Pradesh, India. J Ethnopharmacol 2007;113:54-61.
Albuquerque UP, Monteiro JM, Ramosa MA, Amorim ELC. Medicinal and magic plants from a public market in northeastern Brazil. J Ethnopharmacol 2007;110:76-91.
Israilov IA, Yuhusov MS. Alkaloids of four species of Argemone. Chem Nat Compd 1986;22:189-92.
Tripathi PN, Tripathi M, Pandey VB, Singh D. Alkaloids of Argemone mexicana. Orient J Chem 1999;15:185-6.
Chang YC, Hsieh PW, Chang FR, Wu RR, Liaw CC, Lee KH, et al. Two new protopines argemexicaines A and B and the anti-HIV alkaloid 6-acetonyl dihydro chelerythrine from formazan Argemone mexicana. Planta Med 2003;69:148-52.
Chang YC, Chang FR, Khalil AT, Hsieh PW, Wu YC. Cytotoxic benzophenanthridine and benzylisoquinoline alkaloids from Argemone mexicana. Zeitschrift für Naturforschung 2003;58:521-6.
Sukumar D, Nambi RA, Sulochana N. Studies on the leaves of Agremone mexicana. Fitoterapia 1984;55:325-53.
Usman H, Abdulrahman FI, Usman A. Qualitative phytochemical screening and in vitro antimicrobial effects of methanol stem bark extract of Ficus thonningii (moraceae). Afr J Tradit Complementary Altern Med 2009;6:289-95.
Badami RC, Gunstone FD. Vegetable oils. Examination of component acids of Argemone mexicana seed oil by reversed-phase chromatography. J Sci Food Agric 1962;13:255-7.
Rukmini C. New, unusual long chain fatty acid (argemonic acid) from Argemone mexicana. J Am Oil Chem Soc 1975;52:171-3.
Harborne JB, Williams CA. Flavonoids in the seeds of Argemone mexicana: a reappraisal. Phytochemistry 1983;22:1520-1.
Pathak NKR, Biswas M, Seth KK, Dwivedi SPD, Pandey VB. Chemical investigation of Argemone mexicana. Die Pharmazie 1985;40:202.
Rahman W, Ilyas M. Flower Pigments. Flavonoids from Argemone mexicana L. (Papaveraceae). J Org Chem 1962;27:153-5.
Singh S, Singh TD, Pandey VB. Constituents of argemone species. Indian Chem Soc 2011;88:275-6.
Singh S, Pandey VB, Singh TD. Alkaloids and flavonoids of Argemone mexicana. Nat Prod Res 2012;26:16-21.
Bhardwaj DK, Bisht MS, Jain RK, Munyal A. Phenolics from the seeds of Argemone mexicana. Phytochemistry 1982;21:2154-6.
Singh S, Singh A, Jaiswal J, Singh TD, Singh VP, Pandey VB, et al. Antifungal activity of the mixture of quaternary alkaloids isolated from Argemone mexicana against some phytopathogenic fungi. Arch Phytopathol Pflanzenschutz 2010;43:769-74.
Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. 3rd Edn. Chapman and Hall: London; 1998. p. 302.
Li C, Bu PB, Yue DK, Sun YF. Chemical constituents from roots of ficus hirta. China J Chin Mater Med 2006;31:131-3.
Fleischer TC, Sarkodie JA, KOmlaga G, Kuffour G, Dickson A, Mensah MLK. Hypoglycemic and antioxidant activites of the stem bark of Morinda lucida benth in streptozocin induced diabetic rats. Pharmacogn Commun 2011;1:23-9.
Muhit MA, Tareq SM, Apu AS, Basak D, Islam MS. Isolation and identification of compounds from the leaf extract of Dillenia indica linn. Bangladesh Pharm J 2010;13:49-53.
Habib MR, Nikkon F, Rahman M, Haque ME, Karim MR. Isolation of stigmasterol and beta-sitosterol form methanolic extract of root of bark of Calotropis gigantean (Linn.). Pak J Biol Sci 2007;10:4174-6.
Jain PS, Bari SB. Isolation of stigmasterol and gamma sitosterol form petroleum ether extract of woody stem of Abelmsochus manihot. Asian J Biol Sci 2009;2:112-7.