ISOLATION AND CHARACTERIZATION OF STIGMASTEROL FROM CHLOROFORM FRACTION OF AERIAL PART OF ARGEMONE MEXICANA L
Keywords:Phytosterols, Argemone mexicana, Stigmasterol, Isolation, Papaveraceae
Objective: Phytosterols are group of steroidal alcohol play important roles in structural component in the cell membrane and play a role in membrane stability. There are almost 22 different sterols are found yet and the major phytosterols include ÃŸ-sitosterol, campesterol and stigmasterol. The objective of this study was to isolate and characterize the bioactive principles from the chloroform fraction of Argemone Mexicana L.
Methods: The isolation was done using column chromatography using gradient elution with different mobile phases. The isolated compound was subjected to spectral analysis. Structure elucidation was carried out on basis of spectral analysis.
Results: The chemical investigation of the chloroform fraction of aerial parts of belonging to the family Papaveraceae led to the isolation of stigmasterol. The isolated compounds were characterized using various spectroscopic data as well as chemical studies.
Conclusion: From the spectral characteristics, the isolated compound from the chloroform fraction of aerial parts was confirmed to be stigmasterol. This is the first ever report of these stigmasterol compound from the chloroform fraction of aerial parts of Argemone mexicana.
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