• Surender S. Yadav Maharshi Dayanand University, Rohtak
  • Kuldeep Dahiya Maharshi Dayanand University, Rohtak
  • Showkat A. Ganie Maharshi Dayanand University, Rohtak
  • Surender K. Gulia Maharshi Dayanand University, Rohtak


Aegle marmelos, Antibacterial property, Disc diffusion, Traditional medicine


Objective: To investigate the antibacterial property of Aegle marmelos (L.) Correa leaf extracts.

Methods: The antibacterial property of A. marmelos crude methanolic and chloroform leaf extracts was evaluated through agar disc diffusion method. Two Gram-positive (Bacillus subtilis, Staphylococcus aureus) and three Gram-negative bacteria (Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli) were used for the study.

Results: The zone of inhibitions obtained from the agar diffusion disc method showed that methanolic and chloroform leaf extracts exhibited maximum (15 mm) activity against P. aeruginosa and 18 mm against S. aureus, respectively. Both extracts were partially active against B. subtilis and K. pneumonia. The activity was maximum at higher concentration (100mg/ml) and decreased gradually with the decrease in the concentration of the extracts against all the test pathogens.

Conclusions: From this study, it can be concluded that A. marmelos extracts may be a potential antibacterial agent against both Gram-positive and Gram-negative bacteria. The antibacterial property may be attributed to different phytoconstituents present in the crude methanolic and chloroform extracts.



Download data is not yet available.

Author Biographies

Surender S. Yadav, Maharshi Dayanand University, Rohtak

Department of Botany

Kuldeep Dahiya, Maharshi Dayanand University, Rohtak

Department of Botany

Showkat A. Ganie, Maharshi Dayanand University, Rohtak

Department of Botany

Surender K. Gulia, Maharshi Dayanand University, Rohtak

Department of Botany


Barbour EK, Al-Sharif M, Sagherian VK, Habre AN, Talhouk RS, Talhouk SN. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J Ethnopharmacol 2004;93:1-7.

Ramesh A, Hyma B. Traditional indian medicine in practice in an Indian metropolitan city. In: Geographical aspects of health and diseases in India. New Delhi: Akhtar and Learmonth Concept Publishing Co; 1985.

Eloff JN. Antibacterial activity of 27 Southern African members of the combretaceae. South Afr J Sci 1999;95:148-52.

Dhankhar S, Ruhil S, Balhara M, Dhankhar S, Chhillar AK. Aegle marmelos (Linn.) Correa: a potential source of phytomedicine. J Med Plants Res 2011;5(9):1497-1507.

Singh S. Standardization of processing technology of bael (Aegle marmelos Correa). Thesis, Doctor of philosophy in horticulture, College of Agriculture CCS, HAU, Hisar; 2000.

Okeke MI, Iroegbu CU, Eze EN, Okoli AS, Esimone CO. Evaluation of extracts of the root of Londolphia owerrience for antibacterial activity. J Ethnopharmacol 2001;78:119-27.

Mukherjee PK, Giri SN, Pal M, Saha BP. Antibacterial efficiency of Nelumbo nucifera (Nympheaceae) rhizome extract. Ind Drugs 1996;32:274-6.

Venkatesan D, Karrunakarn CM, Selva SK, Palani PTS. Identification of phytochemical constituents of Aegle marmelos responsible for antimicrobial activity against selected pathogenic organisms. Ethnobot Leaflets 2009;13:1362-72.

Mujeeb F, Bajpai P, Pathak N. Phytochemical evaluation, antimicrobial activity and determination of bioactive components from leaves of Aegle marmelos. Bio Med Res Int 2014;1-11.

Ajaiyeoba E. Phytochemical and antibacterial properties of Parkia biglobosa and Parkia bicolor leaf extracts. Afr J Biomed Res 2002;5(1):125-9.

Mamtha B, Kavitha K, Srinivasan KK, Shivananda PG. An in vitro study of the effect of Centella asiatica (Indian pennywort) on enteric pathogens. Ind J Pharmacol 2004;36(1):41.

Zablotowicz RM, Hoagland RE, Wagner SC. Effect of saponins on the growth and activity of rhizosphere bacteria. Adv Exp Med Biol 1996;405:83-95.

Hemandez NE, Tereschuk ML, Abdala LR. Antimicrobial activity of flavonoids of medicinal plants from Taf´ı del Valle (Tucum´an Argentina). J Ethnopharmacol 2000;73(1-2):317-22.

Epand RF, Savage PB, Epand RM. Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ceragenins). Biochim Biophys Acta 2007;1768(10):2500-09.

Kothari S, Mishra V, Bharat S, Tonpay SD. Antimicrobial activity and phytochemical screening of serial extracts from leaves of Aegle marmelos (linn.). Acta Pol Pharm Drug Res 2011;68(5):687-92.

Tortora GJ, Funke BR, Case CL. Microbiology: An Introduction. Benjamin Cummings, San Francisco; 2001. p. 88.

Kostova I, Dinchev D. Saponins in Tribulus terrestris –chemistry and bioactivity. Phytochem Rev 2005;4:111-37.

Ordonez RM, Ordonez AA, Sayago JE, Moreno MIN, Isla MI. Antimicrobial activity of glycosidase inhibitory protein isolated from Cyphomandra betacea Sendt. fruit. Peptides 2006;27:1187-91.



How to Cite

Yadav, S. S., K. Dahiya, S. A. Ganie, and S. K. Gulia. “ANTIBACTERIAL ACTIVITY OF AEGLE MARMELOS (L) CORREA”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 462-4,



Short Communication(s)