PHYTOCHEMICAL SCREENING, BOTANICAL STUDY AND DNA FINGERPRINTING OF PRUNUS AMYGDALUS BATSCH UMM ALFAHM†CULTIVAR CULTIVATED IN EGYPT
Keywords:
Botanical study, Prunus amygdalus, DNA fingerprinting, Phytochemical screeningAbstract
Objective: The present study was designed to throw light on the phytochemical screening, macro and micro morphological studies, as well as, DNA fingerprinting of Prunus amygdalus Batsch Umm alfahm cultivar with the aim of plant authentication.
Methods: Phytochemical screening of the stems, leaves and pericarps was carried out using standard procedures. DNA fingerprinting was carried out using randomly amplified polymorphic DNA (RAPD) using ten primers for the analysis. In addition, seed protein was analyzed using continuous polyacrylamide gel electrophoresis in vertical slab apparatus in the presence of sodium dodecyl sulfate (SDS-PAGE).
Results: Phytochemical investigation of P. amygdalus revealed positive results for the presence of steam volatile substances and saponins in leaves, while pericarp contains traces of saponins. Whereas, sterols and/or triterpenoids, tannins, carbohydrate and/or glycosides, and flavonoids were detected in leaves and stems and as traces in pericarp.
Conclusion: From the present study, macro and micro morphological characters, as well as, DNA fingerprinting can be considered as the identifying parameters to authenticate the plant.
Downloads
References
Egyptian Ministry of Agriculture statistics; 2009. p. 354.
Namli S, IÅŸikalan Ç, AkbaÅŸ F, BaÅŸaran D. Improved in vitro rooting of almond (Amygdalus communis) cultivar Nonpareil â€. Plant Omics J 2011;4:14-8.
Hartwell JL. Plants used against cancer: a survey. Lloydia; 1971. p. 30–4.
Duke JA, Wain KK. Medicinal plants of the world, computer index; 1981.
Shahidi F, Zhong Y, Wijeratne S, Ho C. Nuts Composition: Phytochemicals and Health Effects. Naturaceutical science and technology. 9th Edition: CRC Press; 2009. p. 127-41.
Brain KR, Turner TD. Practical evaluation of phytopharmaceuticals. 1st ed. Bristol: Wright-Scientechnica; 1975. p. 144.
Ciulei I. Methodology for analysis of vegetable drugs. UNIDO Romania; 1981. P. 17-25.
Harborne JB. Phytochemical methods: a guide to modern technique of plant analysis. London: Chapman and Hall; 1992.
Evans WC, Trease GE. Trease and Evans pharmacognosy. WB London: Saunders Ltd; 2002. p. 32, 33, 95-9, 512, 547.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 32nd ed. Nirali Prakashan; 2005.
Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 1987;19:11-5.
Ruzin S. Plant microtechnique and microscopy. New York: Oxford University Press; 1999.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990;18:6531–5.
Stegmann H, Burgermeister W, Shah AA, Francksen HE, Krogerrecklenfort E. Gel electrophoresis and isoelectric focusing. Braunschweig West Germany: Institut fur Biochemie Biologische Bundesanstlt: Messeweg; 11, D-3300; 1988.
Rohlf FJ. Numerical Taxonomy and Multivariate Analysis. Version 2.1. New York: Applied Biostatistics Inc; 2000.
Al-Rawashdeh IM. Genetic variability in a medicinal plant Artemisia judaica using random amplified polymorphic DNA (RAPD) markers. Int J Agric Biol 2011;13:279-82.
Badr A, Morcy W, Abdelfattah S, Shams S, Shehab A. Genetic diversity in Artemisia monosperma and Artemisia judaica populations in Egypt based on morphological, karyological and molecular variations. J Med Plants Res 2012;6:66-78.