A NOVEL RAPID RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF BALOFLOXACIN IN TABLETS

Authors

  • M. Sindhusri Jawaharlal Nehru technological University, Hyderabad (JNTUH)
  • T. Swetha Jawaharlal Nehru technological University, Hyderabad (JNTUH)
  • A. Ramadevi Jawaharlal Nehru technological University, Hyderabad (JNTUH)
  • A. Ashok Kumar Jawaharlal Nehru technological University, Hyderabad (JNTUH)

Keywords:

Nil, Balofloxacin, Method development, Validation

Abstract

Objective: To develop a rapid, accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method and validate as per ICH guidelines for the quantitative estimation of Balofloxacin in tablets.

Methods: The optimized method uses a reverse phase column, Enable Make C18G (250 X 4.6 mm; 5μ), a mobile phase of triethylammonium phosphate buffer (pH 2.3): acetonitrile in the proportion of 20:80 v/v, flow rate of 1.0 ml/min and a detection wavelength of 280 nm using a UV detector.

Results: The developed method resulted in Balofloxacin eluting at 2.6 min. Balofloxacin exhibited linearity in the range 2.5-7.5μg/ml. The precision is exemplified by relative standard deviation of 1.08%. Percentage Mean recovery was found to be in the range of 98â€102, during accuracy studies.

Conclusion: A rapid, accurate, precise and linear isocratic RP-HPLC method was developed and validated for the quantitative estimation of Balofloxacin in tablets as per ICH guidelines and hence it can be used for routine analysis in various pharmaceutical industries.

 

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Author Biographies

M. Sindhusri, Jawaharlal Nehru technological University, Hyderabad (JNTUH)

Department of Pharmaceutical Analysis and Quality assurance, Student

T. Swetha, Jawaharlal Nehru technological University, Hyderabad (JNTUH)

Department of Pharmaceutical Analysis and Quality assurance, Student

A. Ramadevi, Jawaharlal Nehru technological University, Hyderabad (JNTUH)

Department of Pharmaceutical Analysis and Quality assurance, Student

A. Ashok Kumar, Jawaharlal Nehru technological University, Hyderabad (JNTUH)

Professor and Head of the department of Pharmaceutical Analysis and Quality assurance

 

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Published

01-02-2015

How to Cite

Sindhusri, M., T. Swetha, A. Ramadevi, and A. A. Kumar. “A NOVEL RAPID RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF BALOFLOXACIN IN TABLETS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 2, Feb. 2015, pp. 319-22, https://journals.innovareacademics.in/index.php/ijpps/article/view/3473.

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