PHYTOCHEMICAL ANALYSIS, HEPATOPROTECTIVE AND HPTLC ANALYSIS OF FRACTIONS OF ERYTHROXYLUM MONOGYNUM METHANOLIC LEAF EXTRACT ON PARACETAMOL INDUCED HEPATIC DAMAGE
Keywords:
Erythroxylum monogynum, Hepatoprotection, Paracetamol, Silymarin, HPTLCAbstract
Objective: To investigate the hepatoprotective activity of fractions of E. monogynum methanolic leaf extract against paracetamol induced hepatic damage and perform HPTLC analysis of active fraction.
Methods: Fractions derived from methanolic extract of leaves of E. monogynum (Pet. ether, chloroform and hydroalcoholic) were screened for hepatoprotective activity. Doses of 100mg/kg, 200mg/kg of different fractions administered for seven days and on 8th day toxicity by paracetamol was induced. Levels of biochemical markers along with histopathological changes were monitored to evaluate the extent of hepatoprotection after 24 hrs of toxicity induction.
Results: A significant decrease in the biochemical parameters was evident by the hydroalcoholic fraction as compared to the toxic group which is also confirmed by histopathological changes observed. HPTLC analysis of hydroalcoholic fraction confirmed the presence of rutin, a flavonoidal glycosides besides other phytochemicals.
Conclusion: The results of the present study indicates the significant hepatoprotective activity by the hydroalcoholic fraction of E. monogynum against paracetamol induced toxicity.
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Kamisan FH, Yahya F, Ismail NA, Din SS, Mamat SS, Zabidi Z, et al. Hepatoprotective activity of methanol extract of Melastoma malabathricum leaf in rats. J Acupunct Meridian Stud 2013;6(1):52-5.
Russmann S, Gerd A, Grattagliano I. Current concept of mechanisms in drug induced hepatotoxicity. Curr Med Chem 2009;16:3041–53.
Sheetal V, Singh SP. Current and future status of Herbal medicine. Veterinary World 2008;11:347-50.
Parrotta J. A Healing plants of peninsular India. CAB International; 2001. p. 279-80.
Kirtikar KR, Basu BD. Indian medicinal plant. 2nd ed, Vol I. Dehradun: International Book Distributors; 1987. p. 415.
Sabeena SH, Namdeo AG. Heptoprotective effects of leaves of Erythroxylum monogynum Roxb. on paracetamol induced toxicity. Asian Pac J Trop Biomed 2013;3(11):877-81.
Trease GE, Evans WL. Pharmacognosy. 16th ed. London: Bailliere Tindall Ltd; 2009. p. 60-75.
Jafri MA, Jalis Subhani M, Kalim javed, Surender Singh. Hepatoprotective activity of leaves of Cassia occidentalis against Paracetamol and ethylalchohol intoxofication in rats. J Ethnopharmacol 1999;66:355-61.
Slater TF, Sawyer BC. The stimulatory effects of carbon tetrachloride and other halogenoalkanes or peroxidative reactions in rat liver fraction in vitro. Biochem J 1971;123:805-14.
Morgan MS, Depirre JW, Mannervik B. Levels of glutathione, gulathione reductase and glutathione S-transferase activities in rat lung and liver. Biochem Biophy Acta 1979;582:67-78.
Misera HP, Fridocich. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for SOD. J Biol Chem 1972;3170-5.
Mythili S, Arunachalam S. High performance thin layer chromatography profile of Cassytha filiformis. Asian Pac J Trop Med 2012:S1431-5.
Yen FL, Wu TH, Lin LT, Lin CC. Hepatoprotective and antioxidant effects of Cuscuta chinensis against acetaminophen induced hepatotoxicity in rats. J Ethnopharmacol 2007;111:123-8.
Sivakrishnan S, Kottaimuthu A. Hepatoprotective acitivity of ethanolic extract of aerial parts of Albizia procera Roxb (Benth) against paracetamol induced liver toxicity. Int J Pharm Pharm Sci 2014;6:233-8.
Sathya A, Siddhuraju P. Protective effect of bark and empty pod extracts for Acacia auriculiformis against paracetamol intoxicated liver injury and alloxan induced type II diabetes. Food Chem Toxicol 2013;56:162-70.
Domitrovic R, Jakovac H, Marchesi VV, Knezevic SV, Cvijanovic O, Tadic Z, et al. Differential hepatoprotective mechanism of rutin and quercetin in CCl4 intoxicated BALB/Cn mice. Acta Pharmacol Sin 2012;33:1260-70.