HISTOPATHOLOGICAL ALTERATIONS INDUCED BY COMMON KRAIT BUNGARUS CAERULEUS VENOM ON HEPATIC, RENAL AND CARDIAC TISSUES OF ALBINO MICE
Keywords:
Bungarus caerulus, Cardiac tissue, Hepatic tissue, Histology, Krait, Renal tissue, VenomAbstract
Objective: Bungarus caeruleus (common krait) is a venomous snake in Bangladesh as well as whole over the Indian subcontinent and causing a significant number of mortality due to most of the snakebites in this region. The present study was aimed to evaluate the extinct of damage caused by intraperitoneal introduction of krait crude venom at a sublethal dose on liver, kidney and heart tissues of rodent animal.
Methods: Twelve mice were divided mainly into two groups as control and envenomated group. Lyophilized Bungarus caeruleus venom was dissolved in 0.9% NaCl solution and injected intraperitoneally as dose equivalent to 1/2 LD50 (0.08 mg/Kg) in the mice of envenomared groups. The animals were sacrificed after 24 hour of envenoming for histological study.
Results: Results were indicated inflammatory cellular infiltration, vacuolation in hepatocytes, congestion in the central vain and hemorrhage in hepatic tissue. Renal tissue showed inflammatory infiltration, cytoplasmic vacuolation, glomerular and vessels congestion, shrinking of glomeruli, hemorrhage as well as necrosis of proximal tubules. Cardiac tissue also presented heart-hemorrhage, multifocal areas of myocardial fiber along with separation of the muscle fibers of envenomated groups compared with regular and compact organization of their control respectively.
Conclusion: From these observations, we can be concluded that such injury could be considered among the factors that lead to death caused by Bungarus caeruleus envenoming and by considering the site and the mode of action of this poison on tissue level, this findings may contribute for the discovery of antivenom related valuable pharmaceutical products.
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