• Viveksarathi Kunasekaran Annamalai University
  • Kannan Krishnamoorthy Annamalai University


Solid lipid nanoparticles, Rasagiline mesylate, Compatibility studies, Isothermal stress testing, Scanning electron microscopy


Objective: Compatibility study is an important element that should be performed at the early development stage of stable and effective solid dosage form.

Methods: The compatibility studies of drug with different polymers and surfactant were investigated by using different methods Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC) and Isothermal Stress Testing (IST). The compatibility study of Rasagiline mesylate was performed with Chitosan, Stearic acid and Poloxamer 407. Drug and polymer mixtures were prepared in 1:1 ratio and the compatibility study was performed at25°C±2/60%±5RHand 40°C±2/75%±5RHfor one month. The resulting compatible drug and excipients were used in the formulation of solid lipid nanoparticles by Microemulsion method.

Results: FTIR Spectroscopy results suggest that there is good compatibility between the drug and polymers. DSCresults showed that interaction between Rasagiline mesylate and Chitosan,the other excipients were compatible with RM and the IST study results clearly indicate the stable nature of the Rasagiline mesylate. The prepared RM-SLNs formulation shows a mean particle size within the nanometre range. SEM confirmed the size and shape of RM-SLNs. The results of zeta potential indicates a narrow size distribution. The RM-SLNs were found to be with acceptable morphometric properties, narrow size distribution, high entrapment efficiencyand good stability after 3 months of storage at different conditions (25°C±2/60%±5RH and 40°C±2/75%±5RH).

Conclusion: The FTIR, DSC and IST results shown that Stearic acid and Poloxamer 407were found to be compatible with Rasagilinemesylate. The Rasagiline mesylate loaded nanoscale solid lipid particles was successfully formulated with compatible excipients and it was found to be stable after accelerated stabilitystudies.



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Aulton ME. Pharmaceutics: the scientific principles of dosage form design. 2nd edition. Churchill Livingstone; 2002. p. 136.

Akhilesh Vikarm Singh, Lila Kanta Nath. Evaluation of compatibility of lamivudine with tablet excipients and a novel synthesized polymer. J Mater Environ Sci 2011;2(3):243-50.

Bogdan Tit, Adriana Fulias, Geza Bandur, Eleonora Marian, Dumitru Tit. Compatibility study between ketoprofen and pharmaceutical excipients used in solid dosage forms. J Pharm Biomed Anal 2011;56:221-7.

Picciochi R, Diogo HP, Da Piedade MEM. Thermochemistry of paracetamol. J Therm Anal Cal 2010;9:391-401.

Tita B¸ Fulias A¸ Szabadai Z, Rusu G, Bandur G, Tit D. Compatibility study between ibuprofen and excipients in their physical mixtures. J Therm Anal Cal 2011;105:517-27.

Neto HS, Noak CS, Matos JR. Thermal analysis and compatibility studies of prednicarbate with excipients used in semi solid pharmaceutical form. J Therm Anal Cal 2009;97:367-74.

Tatsuyoshiwakasawa, Kyoko sano, Yutaka hirakura, Toshimasatoyooka, Satoahikitamura. Solid-state compatibility studies using a high throughput and automated forced degradation system. Int J Pharm 2008;355:164-73.

Carlson E, Chandler W, Galdo I, kudla T, Ta C. Automated integrated forced degradation and drug-excipients compatibility studies. J Assoc Lab Autom 2005;10:374-80.

Karin Liltorp, Trine Gorm Larsen, Birgitte Willumsen, Rene Holm. Solid state compatibility studies with tablet excipients using non thermal Methods. J Pharm Biomed Anal 2011;55:424-8.

Corvi Mora P, Cirri M, Mura P. Differential scanning calorimetry as a screening technique in compatibility studies of DHEA extended release formulations. J Pharm Biomed Anal 2006;42:3-10.

Harding L, Qi S, Hill G, Reading M, Craig DQM. The development of microthermal analysis and photo thermal microspectroscopy as novel approaches to drug-excipient compatibility studies. Int J Pharm 2008;354:149-57.

Ceschel GC, Badiello R, Ronchi C, Maffei P. Degradation of components in drug formulations: a comparison between HPLC and DSC methods. J Pharm Biomed Anal 2003;32:1067-72.

Lira AM, Araujo ASS, Bas lio IDJ, Santos BLL, Santana DP, Macedo RO. Compatibility studies of lapachol with pharmaceutical excipients for the development of topical formulations. Thermochim Acta 2007;457:1-6.

Serajuddin ATM, Thakur AB, Ghoshal RN, Fakes MG, Ranadive SA, Morris KR, Varia SA. Selection of solid dosage form composition through drug excipient compatibility testing. J Pharm Sci 1998;88:696-704.

Rascol O, Brooks DJ, Melamed E, Oertel W, Poewe W, Stocchi F, et al. Rasagiline as an adjunct to levodopa in patients with Parkinson’s disease and motor fluctuations (LARGO, lasting effect in adjunct therapy with rasagiline given once daily, study): a randomised, double-blind, parallel-group. Lancet 2005;365:947-54.

Orly Weinreb, Silvia Mandel, Orit Bar-Am, Merav Yogev-Falach, Yael Avramovich-Tirosh, Tamar Amit, et al. Multifunctional neuroprotective derivatives of rasagiline as anti-alzheimer’s disease drugs. Neurother: J Am Soc Exper Neuro Ther 2009;6:163-74.

Youdim MB, Maruyama W, Naoi M. Neuropharmacological, neuroprotective and amyloid precursor processing properties of selective MAO-B inhibitor anti-Parkinsonian drug, rasagiline. Drugs Today 2005;41:369-91.

Blandini, Armentero MT, Fancellu R, Blaugrund E, Nappi G. Neuroprotective effect of rasagiline in a rodent model of Parkinson’s disease. Exper Neuro 2004;187:455-9.

David R, P Guay. Rasagiline (TVP-1012): a new selective monoamine oxidase inhibitor for parkinson's disease. Am J Geri Pharmacother 2006;4(4):331-46.

Irene A Malaty, Hubert H Fernandez. Role of rasagiline in treating Parkinson’s disease: effect on disease progression. Ther Clin Risk Manage 2009;5:413-9.

Lifeng Qi, Zirong Xu, Xia Jiang, Caihong Hu, Xiangfei Zou. Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr Res 2004;339:2693-700.

Santanu Mallik, Mahendra D. Kshirasagar, Vipin S Ani. Studies on physical/chemical compatibility between drugs with various pharmaceutical excipients. Der Pharm Let 2011;3(5):173-8.

Cotton ML, Wu DW, Vadas EB. Drug-excipient interaction study of enalaprilmaleate using thermal analysis and scanning electron microscopy. Int J Pharm 1987;40:129-42.

Doijad RC, Manvi FV, Godhwani DM, Joseph R, Deshmukh NV. Formulation and targeting efficiency of cisplatin engineered solid lipid nanoparticles. Indian J Pharm Sci 2008;70(2):203-7.

Mayur K, Padhi BK, Chougule M, Mishra A. Methotrexate loaded solid lipid nanoparticles for topical treatment of psoriasis: formulation and clinical implication. Drug Deliv Tech 2002;5:1-13.

Viveksarathi K, Kannan K. Multi criteria decision making to select the best method for the preparation of solid lipid nanoparticles of rasagiline mesylate using analytic hierarchy process. J Adv Pharm Technol Res 2014;5(3):1-7.

Barnabas Wilson, Malay Kumar Samanta, Kumaraswamy Santhi, Kokilampal Perumal, Sampath Kumar, Nallupillai Paramakrishnan, et al. Poly (n-butylcyanoacrylate) nanoparticles coated with polysorbate 80 for the targeted delivery of rivastigmine into the brain to treat Alzheimer's disease. Brain Res 2008;1200:159-68.

Kashanian S, Azandaryani AH, Derakhshandeh K. New surface-modified solid lipid nanoparticles using N-glutarylphosphatidylethanolamine as the outer shell. Int J Nanomed 2011;6:2393-401.

Zhinan Mei, Huabing Chen, Ting Weng, Yajiang Yang, Xiangliang Yang. Solid lipid nanoparticle and microemulsion for topical delivery of triptolide. Eur J Pharm Biopharm 2009;56:189-96.

Shuyu Xie, Luyan Zhu, Zhao Dong, Yan Wang, Xiaofang Wang, Wen Zhong Zhou. Preparation and evaluation of ofloxacin-loaded palmitic acid solid lipid nanoparticles. Int J Nanomed 2011;6:547-55.

Sanjay singh, Ajay Kumar dobhal, Achintjain, Jayant Kumar pandit, Subhashis Chakraborty. Formulation and evaluation of solid lipid nanoparticles of a water soluble drug: zidovudine. Chem Pharm Bull 2010;58(5):650-5.

Marcos Fernandez, Sofia Negroa, Karla Slowing, Ana Fernandez-Carballido, Emilia Barcia. An effective novel delivery strategy of rasagiline for Parkinson’s disease. Int J Pharm 2011;419:271-80.

Viveksarathi K, Rajarajan R, Kannan K, Manavalan R. Dosage form design and evaluation of Eperisone hydrochloride matrix film coated extended release tablets. Int J Pharm Pharm Sci 2012;4(2):575-81.

Laith Hamza Samein. Preparation and evaluation of nystatin loaded-solid-lipid nanoparticles for topical delivery. Int J Pharm Pharm Sci 2014;6(2):592-7.

Rajan K Verma, Sanjay Garg. Selection of excipients for extended release formulations of glipizide through drug-excipient compatibility testing. J Pharm Biomed Anal 2005;38:633-44.



How to Cite

Kunasekaran, V., and K. Krishnamoorthy. “COMPATIBILITY STUDIES OF RASAGILINE MESYLATE WITH SELECTED EXCIPIENTS FOR AN EFFECTIVE SOLID LIPID NANOPARTICLES FORMULATION”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 1, Jan. 2015, pp. 73-80,



Original Article(s)