• Muhammad A. Rajput Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan
  • Rafeeq A. Khan Department of Basic Medical Sciences, College of Medicine-Jeddah, King Saud bin Abdulaziz University for Health Sciences
  • Zeeshan Feroz Department of Basic Sciences, College of Science and Health Professions-Riyadh, King Saud bin Abdulaziz University for Health Sciences


Trachyspermum ammi L, Diazepam, Hole board test, Passive avoidance test


Objective: The anxiolytic activity of the methanol extract of Trachyspermum ammi. L was investigated by hole board and passive avoidance response test.

Methods: The study was conducted using hole board and passive avoidance response tests. 21 male albino mice and rats for each hole board test and passive avoidance response test were used respectively. In both the experiments animals were equally divided into three groups; control, given 2% gum tragacanth (10 ml/kg), standard given diazepam (1 mg/kg) and test group given 50 mg/kg extract of Trachyspermum ammi. L. The data were subjected to analysis by taking mean and standard error to the mean using one sample t-test.

Results: The extract of Trachyspermum ammi. L revealed a decrease of 17.43 counts/3 minutes in number of head dips (hole board test) as compared to control, which was almost comparable to diazepam. The decrease in compartment change time (passive avoidance response test) of Trachyspermum ammi L treated animals was 107.2 seconds as compared to control. All of the changes were statistically highly significant.

Conclusion: The Methanol extract of Trachyspermum Ammi. L showed anxiolytic activity may be due to the presence of thymol in it, which has the mechanism similar to that of benzodiazepines but further studies are needed to reach a final conclusion.



Download data is not yet available.


Rauniar GP, Deo S, Bhattacharya SK. Evaluation of anxiolytic activity of tensarin in mice. Kathmandu Univ Med J 2007;18:188-94.

Velotis, Calvin M. Neuroimaging in anxiety disorders. In: Geuze E, Vermetten E, Westenberg HGM, eds. Anxiety disorder research. Nova science publishers; 2005. p. 22.

Kapoor LD. Handbook of Ayurvedic medicinal plants. Boca Raton FL, CRC Press; 1990. p. 102.

Thangam C, Dhananjayan R. Anti-inflammatory potential of the seeds of carum copticum Linn. Indian J Pharmacol 2003;35:388-91.

Hammond JA, Fielding D, Bishop SC. Prospects for plant anthelmintics in tropical veterinary medicine. Vet Res Commun 1997;21:13-28.

Malhotra SK, Vijay OP. Ajowan. In: Peter KV, eds. Hand book of herbs and spices volume 2. Woodhead publishing limited, Cambridge, UK; 2004. p. 107-16.

Parthasarathy VA, Chempakam B, Zachariah TJ. Ajowan. In: Zachariah TJ, eds. Chemistry of spices: CAB International; 2008. p. 312.

Nagalakshmi S, Shankaracharya NB, Naik JP, Rao LJM. Studies on chemical and technological aspects of Ajowan (Tachyspermum ammi (L.) syn. Carum copticum Hiern) seeds. J Food Sci Technol 2000;37:277-81.

National advisory committee for laboratory animal research. Guidelines on the care and use of animals for scientific purposesâ€; 2004.

Chopra RN, Chopra IC, Handa KL, Kapur LD. editors. Indigenous drugs of India 2nd ed. UN. Dhur and Sons, Kolkata; 1958. p. 51-595.

Washington DC. National research council. Guide for the care and use of laboratory Animals national academy Press; 1996. p. 1-7.

Hossain MS, Ahmed M, Islam A. Hypolipidemic and hepatoprotective effects of different fractions of ethanolic extract of immature leaves of Mangifera indica (Linn.) in alloxan induced diabetic rats. IJPSR 2010;1:132-8.

Hejazian SH, Morowatisharifabad M, Mahdavi SM. Relaxant effect of carum copticum on intestinal motility in Ileum of rat. World J Zool 2007;2:15-8.

Madhu A, Keerthi PHV, Singh J, Shivalinge GKP. To evaluate the anti-epileptic activity of aqueous root extract of Hemidesmus Indicus in rats. Arch Pharm Sci Res 2009;1:43-7.

Martinez AL, Dominguez F, Orozco S, Chavez M, Salgado H, Gonzalez M, et al. Neuropharmacological effects of an ethanol extract of the Magnolia dealbata zucc. Leaves in mice. J Ethnopharmacol 2006;106:250-5.

Takeda H, Tsuji M, Matsumiya T. Changes in head-dipping behavior in the hole-board test reflect the anxiogenic and/or anxiolytic state in mice. Eur J Pharmacol 1998;350:21-9.

Xueqin S, Wenqiang Li, Yongfeng Y, Jingping Z, Chengdi J, wei Li, et al. The nuclear factor-к β inhibitor pyrrolidine dithiocarbamate reduces polyinosinic-polycytidilic acid-induced immune response in pregnant rats and the behavioral defects of their adult offspring. Behav Brain Funct 2011;7:50.

Richens A, Perucca E. General principles in drug treatment of epilepsy. In: Laidlaw J, Richens A, Chadwick D, eds. Textbook of epilepsy 4th ed. Edinburgh: Churchill Livingstone Communications; 1993. p. 495-59.

Garcia DA, Bujons J, Vale C, Sunol C. Allosteric positive interaction of thymol with the GABAA receptor in primary cultures of mouse cortical neurons. Neuropharmacol 2006;50:25-35.

Hajhashemi V, Rabbani M, Ghanadi A, Davari E. Evaluation of antianxiety and sedative effects of essential oil of Ducrosia anethifolia in mice. Clinics 2010;65:1037-42.

Satou T, Murakami S, Matsuura M, Hayashi S, Koike K. Anxiolytic effect and tissue distribution of inhaled Alpinia zerumbet essential oil in mice. Nat Prod Commun 2010;5:143-6.



How to Cite

Rajput, M. A., R. A. Khan, and Z. Feroz. “EVALUATION OF ANXIOLYTIC ACTIVITY OF METHANOL EXTRACT OF TRACHYSPERMUM AMMI L”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 69-71, https://journals.innovareacademics.in/index.php/ijpps/article/view/3676.



Original Article(s)