ISOLATION AND PURIFICATION OF ANTIBACTERIAL PRINCIPLE FROM AVICENNIA MARINA L IN METHANOL
Keywords:
Avicennia marina Stem, Methanol, 2-propenoicacid, 3-(4-hydroxy-3-methoxyphenyl)- Antibacterial activityAbstract
Objective: The antibacterial principle of Avicennia marina L stem extract was determined by agar well diffusion method followed by GC-MS,1H NMR and 13C NMR.
Methods: Methanol was used as the solvent for the isolation of bioactive principle from the stem of Avicennia marina L. Agar well diffusion method was used to screen the antibacterial activity and FRAP method was employed to determine the antioxidant activity for raw and crude extract as well as the purified compound. GC-MS followed by 1H NMR and 13C NMR were used to elucidate the compound responsible for the antibacterial and antioxidant activity.
Results: The degree of antioxidant and antibacterial activity differs between raw extract, crude extract and the pure compound. The antioxidant activity is more crude extract (p<0.05) and the antibacterial activity is more of pure compound (p<0.05) than the standard antibiotic gentamicin. GC-MS followed by 1H NMR and 13C NMR revealed that the compound is 2-propenoicacid, 3-(4-hydroxy-3-methoxyphenyl)- also called ferulic acid.
Conclusion: The stem extract in methanol shows potential antibacterial and antioxidant activity. It is due to presence of ferulic acid in methanol extract in 14th fraction.
Â
Downloads
References
Mazid M, Kham TM, Mohammad F. role of secondary metabolites in defense mechanism of plants. Biol Med 2011;3(2):232-49.
Van Etten HD, Mansfield JW, Bailey JA, Farmer EE. Two classes of plant antibiotics: phytoalexins versus phytoanticipins. Plant Cell 1994:6:1191-2.
Sabhat saeed, Asma naim, Perween tariq. In-Vitro antibacterial activity of Peppermint. Pak J Bot 2006;38(3):869-72.
N Sharief Md, A Srinivasulu, P Satya veni, Uma Maheswara Rao. Quantification of phytochemicals and antibacterial activity of fruit extracts of AO. Asian J Pharm Clin Res 2014;7:127-30.
Choudhury SA Sree, SC Mukherjee, P Patnaik, M Bapuji. In Vitro antibacterial activity of extracts of selected marine algae and mangroves against fish pathogens. Asian Fish Sci 2005;18:285-94.
PD Abeysinghe, RP Wanegatunge, RN Pathirana. Evaluation of antibacterial activity of different mangrove plant extracts. Ruhuna J Sci 2006;1:104-12.
Benzie IFF, Strain J. J Ferric reducing/antioxidant power assay direct measure of total antioxidant activity of biological fluids and modified version of simultaneous measurement of antioxidant power and ascorbic acid concentration. Methods Enzymol 1999;29:15-27.
Agoramoothy G, Chandrasekaharan M, Venkateswrulu V, Hsu Mj. Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your eye mangrove from India. Braz J Microbiol 2007;38:739-42.
Benerjee D, Chakrabarti S, Hazra AK, Banerjee S, Ray J, Mukherjee B. Antioxidant activity and total phenolics of some mangrove in Sundurbans. Afr J Biotechnol 2008;7:805-10.
Ughari JH, Elmahmood AM, Mazara S. Studies on the antibacterial activity of root extracts of Carica papaya L. Afr J Microbiol Res 2007;37-41.
R Shanmugapriya, T Ramanathan, G Renugadevi. Phytochemical characterization and antimicrobial efficiency of mangrove plants avicennia marina and avicennia officinalis. Int J Pharm Biol Arch 2012;3(2):348-51.
N Sharief Md, A Srinivasulu, P Satya veni, Uma Maheswara Rao. Quantification of tannins, antioxidant capacity and
antibacterial ativities of stem extracts of Avicennia marina L. Int J Biol Med Res 2014;5(1):3869-73.
Proestos C, Boziaris IS, Nychas GJE, Komaitis M. Analysis of flavonoids and phenolic acids in Greek aromatic plants: investigation of their antioxidant capacity and antimicrobial activity. Food Chem 2006;95:664-71.
Roman Merkl, Iveta Hradkova, Vladimir Filip, Jam smi drkal. Antimicrobial and antioxidant properties of phenolic acids alkyl esters. Czech J Food Sci 2010;28(4):275–9.
Christina E, Maddox Lisa M, Laur Li Tian. Antibacterial activity of phenolic compounds against the phytopathogen xylella fastidiosa. Curr Microbiol 2010;60:53–8.
MJ Alves, ICFR Ferreira, HJC Froufe, RMV Abreu, A Martins M. Pintado. Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. J Appl Microbiol 2013;15(2):346-57.
V Vinod Prabhu, C Guruvayoorappan. Phytochemical screening of the methanolic extracts of mangrove Avicennia marina (Forssk) Vierh. Der Pharm Sin 2012;3(1):64-70.
Sun Yu, Ding Yi, Lin Wen-han. Isolation and identification of compounds from marine mangrove plant Avicennia marina. J Peeking University (Health Sci) 2009;41(2):221-5.
Ozhinjampara r Mahendran, mohamed kreir, Helge weingart, Niels fert ig, Mathias Winterhalter. Permeation of antibiotics through Escherichia coli Ompf and ompc porins: screening for influx on a single-molecule level. J Biomol Screening 2010;15:302-7.
Ian-Zhi Li, Hiroshi Nikaido. Efflux-mediated drug resistance in bacteria: an update. Drugs 2009;69:1555–623.