IN VITRO ANTIOXIDANT AND RADICAL SCAVENGING ABILITIES OF AQUEOUS METHANOLIC EXTRACTS OF CASSIA OBTUSA L. PLANT PARTS (CAESALPINIACEAE)
Keywords:
Cassia obtusa, Antioxidant, Aqueous methanolic extract, Free radical scavenging activityAbstract
Objective: The present study was carried out to examine the in vitro antioxidant activity of the aqueous methanolic extracts of Cassia obtusa plant parts.
Methods: The activity was assessed using various in vitro assay models viz., ferric reducing antioxidant power, DPPH•, OH•, O2-, ABTS•+ scavenging activity and lipid peroxidation inhibiting activities. The results were substantiated with well known natural as well as synthetic antioxidants.
Results: Among the samples analyzed, C. obtusa leaf extracts determined (P<0.05) effective antioxidant power in terms of all the assays tested.
Conclusion: Based on these findings, C. obtusa leaf can be considered as a potent source of nutraceutical contributor which could offer protection and ameliorate oxidative stress-induced physiological malfunctions.
Downloads
References
Aruoma OI. Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem Soc 1998;75:199-212.
Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature 2000;408:239-47.
Thenmozhi K, Karthika K, Manian S, Paulsamy S. Studies on in vitro antioxidant potential of pod and seed parts of Bauhinia malabarica Roxb. Asian J Biomed Pharm Sci 2014;4(32):48-56.
Karthika K, Thenmozhi K, Paulsamy S, Manian S. Quantification of phytochemicals and antioxidant potential of various solvent extracts of certain species of Acanthaceae. Int J Green Pharm 2014;8(1):58-64.
Neergheen VS, Bahorun T, Jen LS, Aruoma OI. Bioefficacy of Mauritian endemic medicinal plants: assessment of their phenolic contents and antioxidant potential. Pharm Biol 2007;45:9-17.
Sekar M, Rajendra Prasad KJ, Siddhuraju P, Janardhanan K. New anthraquinones from Cassia obtusa. Fitoterapia 1999;70(3):330-2.
Kirtikar KR, Basu BA. Indian Medicinal Plants. 2nd edition. Periodical Experts Book Publication, New Delhi; 1991. p. 856-60.
Blios MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;26:1199-200.
Klein SM, Cohen G, Cederbaum AI. Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochem 1991;20:6006-12.
Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971;44:276-7.
Taga MS, Miller EE, Pratt DE. Chia seeds as a source of natural lipid antioxidants. J Am Oil Chem Soc 1984;61:928-31.
Siddhuraju P, Manian S. The antioxidant activity and free radical scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds. Food Chem 2007;105:950-8.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26:1231-7.
Pulido R, Bravo L, Sauro-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem 2000;48:3396-402.
Mandal P, Misra TK, Ghosal M. Free radical scavenging activity and phytochemical analysis in the leaf and stem of Drymaria diandra Blume. Int J Integr Biol 2009;7(2):80-4.
Siddhuraju P, Mohan PS, Becker K. Studies on the antioxidant activity of Indian laburnum (Cassia fistula L.): A preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. Food Chem 2002;79:61-7.
Gutteridge JMC. Free radicals in disease processes: A compilation of cause and consequence. Free Radic Res Com 1993;19:141-58.
Sowndhararajan K, Jince Mary Joseph, Dharmar Rajendrakumaran, Sellamuthu Manian. In vitro antioxidant characteristics of different parts of Melothria maderaspatana (L.) Cogn. Int J Pharm Sci 2010;2(3):111-23.
Yen GC, Duh PD, Chuang DY. Antioxidant activity of anthraquinones and anthrone. Food Chem 2000;70:437-41.
Yang JX, Guo J, Yuan JF. In vitro antioxidant properties of rutin. Lebensm Wiss Technol 2008;41:1060-6.
Singh V. Monograph on Indian subtribe Cassiinae (Caesalpiniaceae), Scientific Publisher, India; 2001. p. 1-10.
Chung KT, Wong TY, Huang YW, Lin Y. Tannins and human health: a review. Crit Rev Food Sci Nutr 1998;38:421–64.
Gulcin I, Zubeyr Huyut, Mahfuz Elmastas, Hassan Y Aboul-Enein. Radical scavenging and antioxidant activity of tannic acid. Arabian J Chem 2010;3:43-53.
Perry G, Raina AK, Nonomura A, Wataya T, Sayre LM, Smith MA. How important is oxidative damage? Lessons from Alzheimer’s disease. Free Radic Biol Med 2000;28:831-4.
Arts IC, Hollman PC, Feskens EJ, Bueno de Mesquita HB, Kromhout D. Catechin intake might explain the inverse relation between tea consumption and ischemic heart disease: The zutphen elderly study. Am J Clin Nutr 2001;74:227-32.
Takabe W, Niki E, Uchida K, Yamada S, Satoh K, Noguchi N. Oxidative stress promotes the development of transformation: Involvement of a potent mutagenic lipid peroxidation product, acrolein. Carcinog 2001;22:935-41.
Atmani D, Chaher N, Berboucha M, Ayouni K, Lounis H, Boudaoud H, Debbache N, Atmani D. Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chem 2009;112:303-9.
Mathew S, Abraham TE. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food Chem Toxicol 2006;44:198-206.
Middleton P, Stewart F, Al-Qahtani S, Egan P, Orourke C, Abdulrahman A, et al. Antioxidant, antibacterial activities and general toxicity of Alnus glutinosa, Fraxinus excelsior and Papaver rhoeas. Int J Prod Res 2005;4:81-6.
Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchand NT, Hartz Feld PW, Riechel TL. High molecular weight plant polyphenolics (tannins) as biological antioxidants. J Agric Food Chem 1998;46:1887-92.
Zhu QY, Hackman RM, Ensunsa JL, Holt RR, Keen CL. Antioxidative activities of oolong tea. J Agric Food Chem 2002;50:6929–34.