BIOSYNTHESIS AND CHARACTERIZATION OF INTRACELLULAR TIO2NANOPARTICLES BY LACTOBACILLUS SP: AND ITS POTENTIAL APPLICATION IN DECOLOURIZATION OF METHYL ORANGE DYES
Keywords:
TiO2 nanoparticle, Lactobacillus, Decolourisation of dyesAbstract
Objective: Nanotechnology is emerging as one of the most important and revolutionizing areas in the research field. Nanoparticles are produced by various methods like physical, chemical, mechanical and biological. Biological methods of microorganisms are often preferred because they are clean, non-toxic, safe, biocompatible and environmentally acceptable.
Methods: In the present studies isolation and characterization of Lactobacillus species (LAB) from Curd was done with MRS agar (Hi Media, India. M614). All the isolates were subjected to cell morphology, physiology and an array of biochemical characterization (BCT). The confirmed LAB was used for the TiO2 nanoparticles synthesis. The TiO2 nanoparticles were then characterized by Fourier Transform Infra-Red Spectroscopy (FTIR), Scanning Electron Microscope (SEM).
Results: The SEM images of samples revealed that the nanoparticles were spherical, irregularly shaped with no definite morphology. Biosynthesized TiO2 nanoparticles were ranged in size from 20 to 50 nm.
Conclusion: The presence of TiO2 nanoparticle was confirmed by face centered cubic structure of synthesized TiO2 nanoparticle was confirmed by FTIR pattern.
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