THERAPEUTIC POTENCY OF SWEET ORANGE JUICE OVER LIVER GENOTOXICITY INDUCED BY PESTICIDE LANNATE IN RATS
DOI:
https://doi.org/10.22159/ijpps.2020v12i11.39332Keywords:
Lannate, Micronucleus, Fragmentation, Orange juice, Carbamate pesticidesAbstract
Objective: The aim of the present investigation was to study the potential effect of sweet orange juice against liver genotoxicity induced by lannate.
Methods: adult 36 female rats were divided into 6 groups: group C (control group), group L (lannate group) injected intraperitoneal (i. p.) with 1 mg/kg b. wt. lannate for one day, group J (orange juice group) orally administered a dose of 0.1 ml/10 g b. wt. of orange juice for 48 h, group J+L received the orange juice prior to lannate, group J with L received lannate in continuous with the orange juice and group L+J received lannate prior to the orange juice. Tested parameters were DNA fragmentation, micronucleus, histopathology examination and biochemical analysis.
Results: it was found that, the intake of lannate caused high DNA fragmentation and significant increase (P<0.001) in the number of micronucleated polychromatic erythrocytes in the bone marrow. Furthermore, lannate exhibited some pathological changes in the liver tissues as well as a significant (P<0.001) decrease in the total antioxidative capacity (TAC) and a significant increase in the total oxidative capacity (TOC). On the other hand, orange juice administration of all treatments (pre-treatment, continuous and post-treatment) gave some amelioration against liver damage induced by lannate. While the best results were evidenced in the continuous treatment group where the juice could attenuate liver DNA fragmentation and significantly decreased (P<0.001) the number of micronucleated polychromatic erythrocytes. In addition, it improved the induced degenerative histopathological changes as well as ameliorated the changes occurred in TAC and TOC significantly (P<0.001).
Conclusion: the antigenotoxic impact of orange juice against lannate was therapeutic and hence can counteract the poisonous effect of the pesticide in people who exposed to it.
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Meng X, Li Y, Li S, Gan RY, Li HB. Natural products for prevention and treatment of chemical-induced liver injuries. Compr Rev Food Sci F 2018;17:472-95.
Ojha A, Yaduvanshi SK, Pant SC, Lomash V, Srivastava N. Evaluation of DNA damage and cytotoxicity induced by three commonly used organophosphate pesticides individually and in mixture, in rat tissues. Environ Toxicol 2013;28:543–52.
Rasolonjatovoa MA, Cemeka M, Cengizb MF, Ortaça D, Konuka HB, Karamana E, et al. Reduction of methomyl and acetamiprid residues from tomatoes after various household washing solutions. Int J Food Prop 2017;20:2748–59.
World Health Organization WHO. Methomyl. IPCS, Environmental health criteria. Geneva; 1996. p. 33.
Seleem AA. Teratogenicity and neurotoxicity effects induced by methomyl insecticide on the developmental stages of Bufo arabicus. Neurotoxicol Teratol 2019;72:1–9.
Dong MH, Krieger RI, Ross JH. Calculated reentry interval for table grape harvesters working in california vineyards treated with methomyl. Bull Environ Contam Toxicol 1992;49:708-14.
World Health Organization and International Programme on Chemical Safety. The WHO recommended classification of pesticides by hazard and guidelines to classification 2009. World Health Organization; 2010.
Van Scoy AR, Yue, Deng MX, Tjeerdema RS. Environmental fate and toxicology of methomyl. Rev Environ Contam Toxicol 2013;222:93–109.
Patil JA, Patil AJ, Govindwar SP, Sontakke AV. Protection of liver Injury by vitamin c, e and gsh after methomyl toxicity in rat. J Pharm Chem Biol Sci 2016;3:506-17.
Yi MQ, Liu HX, Shi XY, Liang P, Gao XW. Inhibitory effects of four carbamate insecticides on acetylcholinesterase of male and female carassius auratus in vitro. Comp Biochem Physiol 2006;143:113-6.
Guanggang X, Diqiu L, Jianzhong Y, Jingmin G, Huifeng Z, Mingan S, et al. Carbamate insecticide methomyl confers cytotoxicity through DNA damage induction. Food Chem Toxicol 2013;53:352-8.
Bolognesi C, Peluso M, Degan P, Rabboni R, Munnia A, Abbondandolo A. Genotoxic effects of the carbamate insecticide, methomyl II. In vivo studies with pure compound and the chemical formulation lannate 25. Environ Mol Mutagen 1994;24:235-42.
Wei LY, Chao JS, Hong CC. Assessment of the ability of prpoxur, methomyl, and aldicarb, three carbamate insecticides, to induce micronuclei in vivo in BALB/C mice. Environ Mol Mutagen 1997;29:386-93.
Lin CM, Wei LY, Wang TC. The delayed genotoxic effect of N-nitroso Npropoxur insecticide in mammalian cells. Food Chem Toxicol 2007;45:928-34.
da Silvaa SAV, Clementea A, Rochaa J, Direito R, Marquesa HC, Sepodesa B, et al. Anti-inflammatory effect of limonin from cyclodextrin (un)processed orange juices in in vivo acute inflammation and chronic rheumatoid arthritis models. J Funct Food 2018;49:146-53.
Okwu DE, Emenike IN. Evaluation of the phytonutrients and vitamins content of citrus fruits. Int J Mol Med Adv Sci 2006;2:1-6.
Mehl F, Marti G, Boccard J, Debrus B, Merle P, Delort E, et al. Differentiation of lemon essential oil based on volatile and non-volatile fractions with various analytical techniques: a metabolomic approach. Food Chem 2014;143:325–35.
Roger GDP. Encyclopedia of medicinal plants, Education and Health. Library editorial safeliz S. L. Spain 2005;153-154:265-7.
Alam MA, Subhan N, Rahman MM, Uddin SJ, Reza HM, Sarker SD. Effect of citrus flavonoids, naringin and naringenin, on metabolic syndrome and their mechanisms of action. Adv Nutr 2014;5:404-17.
Aschoff JK, Kaufmann S, Kalkan O, Neidhart S, Carle R, Schweiggert RM. In vitro bioaccessibility of carotenoids, flavonoids, and vitamin c from differently processed oranges and orange juices [Citrus sinensis (L.) Osbeck]. J Agric Food Chem 2015;63:578-87.
Graciela OR, Fredy Y, Betzabe SF, Lilibeth C. Volatile fraction composition of Venezuelan sweet orange essential oil (Citrus sinensis (L.) Osbeck). Ciencia 2003;11:55-60.
Kangralkar VA, Gavimath CV, Venkatesh V, Gowri B, Vinay RH, Priya M. Protective effect of essential oils of citrus reticulata on isoniazid induced hepatotoxicity in wistar rats. Int J Pharm Appl 2010;1:59-61.
Sarkar P, Thirumurugan K. Modulatory functions of bioactive fruits, vegetables and spices in adipogenesis and angiogenesis. J Funct Foods 2019;53:318-36.
Fakhr IMI, El-Dardery M, Mostafa IY, Zayed SMAD. Short term toxicity studies of 'Dursban' and 'Gardona' in mice. Egypt Med Assoc 1982;65:71.
Fahmy MA. Assessment of the genotoxicity of the insecticides: 'Trichlorphon' 'Malathion' and 'Sevin' in the mouse. PhD Thesis, Department of Zoology, Faculty of Science, Ain Shams University, Egypt; 1990.
Amer SM, Fahmy MA, Donya SM. Cytogenetic effect of some insecticides in mouse spleen. J Appl Toxicol 1996;16:1-3.
Franke SIR, Pr D, Erdtmann B, Henriques JAP, Silva JD. Influence of orange juice over the geotoxicity induced by alkylating agents: an in vivo analysis. Mutagenesis 2005;20:279-83.
Bortner CD, Oldenburg NBE, Cidlowski JA. The role of DNA fragmentation in apoptosis. Trends Cell Biol 1995;5:21-6.
Adler ID. Cytogenetic tests in mammals. Venitt S, Parry JM. (eds) Mutagenicity testing (a practical approach). IRI Press: Oxford; 1984. p. 275-306.
Banchroft JD, Stevens A, Turner DR. Theory and practice of histological techniques. Fourth Ed. Churchil Livingstone, New York: London, San Francisco, Tokyo; 1996.
Flohe L, Gunzler WA. Oxygen radicals in biological systems. Methods Enzymol 1984;105:114-212.
Cao G, Alessio H, Cutler R. Oxygen radical absorbance capacity assay for antioxidants. Free Radic Biol Med 1993;14:303-11.
Chan YH. Biostatistics102: quantitative data–parametric and non-parametric tests. Singapore Med J 2003;44:391-6.
Süsse H, Müller H. Pesticide analysis by micellar electrokinetic capillary chromatography. J Chromatography A 1996;730:337-43.
Hammam FM, Abd El Mottaleb EM. Studies of the genotoxic and histopathological effects of the organophosphorous insecticide 'Profenofos' on white rats. Egypt J Hosp Med 2007;29:685-706.
Bonatti S, Bolognesi C, Degan P, Abbondandolo A. Genotoxic effects of the carbamate insecticide methomyl. I. in vitro studies with pure compound and the technical formulation "Lannate 25". Environ Mol Mutagen 1994;23:306-11.
Heikal TM, Mossa ATH, Khalil WKB. Protective effects of vitamin c against methomyl-induced injures on the testicular antioxidant status and apoptosis-related gene expression in rat. J Anal Toxicol 2014;5:1-7.
Niesink RJM, Vries JD, Hollinger MA. Toxicology: principles and applications. C RC Press: INC; 1996.
Fenech M, Ferguson L. Vitamins/minerals and genomic stability in humans. Mutat Res 2001;475:1-6.
Halliwell B. Vitamin C and genomic stability. Mutat Res 2001;475:29-35.
Edenharder R, Krieg H, K€ottgen V, Platt KL. Inhibition of clastogenicity of benzo[a]pyrene and of its trans-7,8-dihydrodiol in mice in vivo by fruits, vegetables, and flavonoids. Mutat Res 2003;537:169-81.
Vijayalaxmi KK, Venu R. In vivo anticlastogenic effects of L-ascorbic acid in mice. Mutat Res 1999;438:47-51.
Cooke MS, Evans MD, Podmore ID, Herbert KE, Mistry N, Mistry P, et al. Novel repair action of vitamin C upon in vivo oxidative DNA damage. FEBS Lett 1998;439:363-7.
Schwedhelm E, Maas R, Troost R, Boger RH. Clinical pharmacokinetics of antioxidants and their impact on systemic oxidative stress. Clin Pharmacokinet 2003;42:437-59.
Yu MH. Environmental toxicology: impacts of environmental toxicants on living systems. 1st edn. Lewis Publishers, USA; 2000. p. 255.
Tassaneeyakul W, Vannaprasaht S, Yamazoe Y. Formation of omeprazole sulphone but not 5-hydroxymeprazone is inhibited by grapefruit juice. Br J Clin Pharmacol 2000;49:139-44.
Edenharder R, Sager JW, Glatt H, Muckel E, Platt KL. Protection by beverages, fruits, vegetables, herbs, and flavonoids against genotoxicity of 2-acetylaminofluoreno and 2-amino-1-methyl-6-phenylimidazol [4,5-6] pyridine (PhIP) in metabolically competent V79 cells. Mutat Res 2002;521:57-72.
Kelly C, Jewell C O’Brien NM. The effect of dietary supplementation with the citrus limonoids, limonin and nomilin on xenobiotic-metabolizing enzymes in the liver and small intestine of the rat. Nutr Res 2003;23:681-90.
Doostdar H, Burke D, Mayer RT. Bioflavonoids: selective substrates and inhibitors for cytochrome P450 CYP1A and CYP1B1. Toxicol 2000;144:31-8.
Abalea V, Cillard J, Dubos MP, Sergent O, Cillard P, Morel I. Repair of iron-induced DNA oxidation by the flavonoid myricetin in primary rat hepatocyte cultures. Free Radic Biol Med 1999;26:1457-66.
Heddle JA, Cimino MC, Hayashi M, Romagna F, Shelby MD, Tucker JD, et al. Micronuclei as an index of cytogenetic damage: past, present, and future. Environ Mol Mutagen 1991;18:277-91.
Peluso M, Munnia A, Bolognesi C, Parodi S. 32P-postlabeling detection of DNA adducts in mice treated with the herbicide roundup. Environ Mol Mutagen 1998;31:55-9.
Hassanane MM, Kassem SM, Safinaz E, Eltoukhy SE. Orange juice reduced genetic and biochemical alterations induced by herbicide Glyphosate in mice. Cell Biol 2014;2 Suppl a14:1-7.
Proteggente AR, Pannala AS, Paganga G, Van Buren L, Wagner E, Wiseman S, et al. The antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin C composition. Free Radical Res 2002;36:217-33.
Del Caro A, Piga A, Vacca V, Agabbio M. Changes of flavonoids, vitamin C and antioxidant capacity in minimal lyprocessed citrus segments and juices during storage. Food Chem 2004;84:99-105.
Sentandreu E, Navarro JL, Sendra JM. Effect of technological processes and storage on flavonoids content and total cumulative fast-kinetics and cumulative slow-kinetics antiradical activities of citrus juices. Eur Food Res Technol 2007;225:905-12.
Tabart J, Kevers C, Pincemail J, Defraigne JO, Dommes J. Comparative antioxidant capacities of phenolic compounds measured by various tests. Food Chem 2009;113:1226-33.
Khan SM. Protective effect of black tea extract on the levels of lipid peroxidation and antioxidant enzymes in liver of mice with pesticide induced liver injury. Cell Biol Funct 2006;24:332-72.
Alharbi FK. Effect of methomyl on fetal development and histological changes in female rats. Egypt J Chem Environ Health 2017;3:110-23.
Mansour SA, Ali AR, Mohamd RI. Ameliorating effect of green tea, sage, and their mixture against methomyl-induced physiological, biochemical, and histopathological alterations in male rats. Egypt Pharmaceut J 2018;17:223-36.
Ke ZL, Pan Y, Xu XD, Nie C, Zhou ZQ. Citrus flavonoids and human cancers. J Food Nutr Res 2015;3:341-51.
Chelly M, Chelly S, Ben Salah H, Athmouni K, Bitto A, Sellami H, et al. Characterization, antioxidant and protective effects of edible Rumex roseus on erythrocyte oxidative damage induced by methomyl. J Food Meas Charact 2020;14:229-43.
Ogunro PS, Ogungbamigbe TO, Ajala MO, Egbewale BG. Total antioxidant status and lipid peroxidation in HIV-1 infected patients in a rural area of south western Nigeria. Afr J Med Sci 2005;24:221-5.
Chabane K, Khene MA, Zaida F, Ainouz L, Giaimis J, Mameri SS, et al. Subacute and subchronic methomyl exposure induced toxic effects on intestines via oxidative stress in male albino rats: biochemical and histopathological study. Drug Chem Toxicol 2020. https://doi.org/10.1080/01480545.2020.1727496.
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