NEW DEVELOPED AND VALIDATED SPECTROSCOPIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TERBINAFINE HYDROCHLORIDE AND FLUCONAZOLE

BHUMIKA THAKUR; INDER KUMAR

doi:10.22159/ijpps.2020v12i11.39344

Authors

BHUMIKA THAKUR Shiva Institute of Pharmacy, Bilaspur, HP, India
INDER KUMAR School of Pharmacy, Abhilashi University, Mandi, HP, India https://orcid.org/0000-0002-7295-5450

DOI:

https://doi.org/10.22159/ijpps.2020v12i11.39344

Keywords:

Fluconazole, Terbinafine hydrochloride, UV-visible spectroscopy, Simultaneous estimation

Abstract

Objective: A new, simple, precise, accurate, and reproducible method or simultaneous equation method was developed and validated for the simultaneous estimation of terbinafine hydrochloride (TH) and fluconazole (FLZ) in pure form.

Methods: Simultaneous estimation of terbinafine hydrochloride and fluconazole was estimated by the ultraviolet (UV) spectrophotometry method. The method was based on the measurement of absorbance at two wavelengths 222 nm and 239 nm, of terbinafine hydrochloride and fluconazole in 0.1N HCl respectively.

Results: Calibration curves terbinafine hydrochloride and fluconazole were found to be linear in the concentration ranges of 0.5-3.0 μg/ml and 80-400 μg/ml, respectively, with their correlation coefficient values (R²) 0.999 and 0.998. LOD and LOQ of TH were found to be 0.067, 0.203 at 222 nm and 0.175, 0.531 at 239 nm, similarly for FLZ; 31.089, 94.210 at 222 nm and 94.380, 286.00 at 239 nm respectively. In the precision study, the % RSD value was found within limits (%). The percentage recovery at various concentration levels varied from 98.50 % to 103.96 % for TH and 97.27 % to 103.83 % for FLZ confirming that the expected method is accurate.

Conclusion: It could be concluded from the results obtained in the present study that this method for simultaneous estimation of TH and FLZ in pure is simple, precise, and economical. The proposed method can be applied successfully for the simultaneous estimation of TH and FLZ in the pure and pharmaceutical dosage form.

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