NOVEL FINDINGS OF PHOENIX DACTYLIFERA, DATES FRUIT EARLY-STAGE HABABUK FOR PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND CYTOTOXIC POTENTIALS
DOI:
https://doi.org/10.22159/ijpps.2021v13i5.39469Keywords:
Date fruit, Ethanolic extract, Antioxidant, Hababuk, Brine shrimp, Phytochemical screeningAbstract
Objective: The present study aims to investigate the phytochemical constituents, antioxidant, and cytotoxic activity of the total ethanolic extract of the hababuk stage of date’s fruits Phoenix dactylifera (PDHE).
Methods: Extracts PDHE obtained with shade dried and a powdered sample of hababuk stage of date fruits Phoenix dactylifera and subjected to phytochemical screening, in vitro antioxidant activity study by DPPH assay and ABTS assay, in vitro cytotoxic studies carried out for three different cell lines Lung Cancer (A549), Human cervical carcinoma (HeLa) and Breast Cancer (MCF)-7 cells by MTT method and brine shrimp lethality assay using standard procedure.
Results: The phytochemical study revealed the presence of important constituents like alkaloids, glycosides, phenolic, flavonoids, carbohydrate, proteins, amino acids, terpenoids, steroids, and saponins. The antioxidant studies of the PDHE compared with standard showed very excellent results IC50 values for DPPH activity and nitric oxide. A good result was also shown by ABTS when compared with that of standard. The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. The extract showed excellent LC50 value, which correlates with cytotoxicity studies previously established. The MTT study results using the three different cell lines compared with the standard doxorubicin, promising results obtained for HeLa cell lines when compared with other cell lines.
Conclusion: Results obtained from the present work indicated that the hebabuk stage of date fruit is a promoising antioxidant and anticancer agent. Further study should be carried out to isolate the bioactive compound responsible for the activity.
Downloads
References
Raina H, Soni G, Jauhari N, Sharma N, Bharadvaja N. Phytochemical importance of medicinal plants as potential sources of anticancer agents. Turkish J Bot 2014;38:1027–35.
Shakya AK. Medicinal plants: future source of new drugs. Int J Herb Med 2016;4:59–64.
Pradeep A, Dinesh M, Govindraj A, Vinothkumar D, Ramesh Babu N. Phytochemical analysis of some important medicinal plants. Int J Biol Pharm Res 2014;5:48–50.
Al-Mamary M, Al-Habori M, Al-Zubairi AS. The in vitro antioxidant activity of different types of palm dates (Phoenix dactylifera) syrups. Arab J Chem 2014;7:964–71.
Ali A, Waly M, Essa MM, Devarajan S. Nutritional and medicinal value of date fruit. Dates Prod Process Food Med Values 2014;1:361–71.
Abdul Hamid NA, Mustaffer NH, Maulidiani M, Mediani A, Ismail IS, Tham CL, et al. Quality evaluation of the physical properties, phytochemicals, biological activities and proximate analysis of nine Saudi date palm fruit varieties. J Saudi Soc Agric Sci 2020;19:151–60.
Hadrami A El, Al-khayri JM. Socioeconomic and traditional importance of date palm. Emirates J Food Agric 2012;24:371–85.
Barzin G, Entezari M, Hashemi M, Hajiali S, Ghafoori M, Gholami M. Survey of antimutagenicity and anticancer effect of Phoenix dactylifera pollen grains. Adv Environ Biol 2011;5:3716–8.
Sarker SD, Nahar L. Chemistry for pharmacy students: general, organic, and natural product chemistry. John Wiley and Sons Ltd T. editor. Chemistry for Pharmacy Students: General, Organic, and Natural Product Chemistry. British Library Cataloguing in Publication Data; 2013. p. 1–383.
CK. Kokate PSBG. Pharmacognosy. 45th ed. Nirali Prakashan; 2010. p. 108–9.
Yusuf, Shakkeela KKS. GC-MS analysis of oil isolated and antioxidant activity of shorea robusta oleoresin. Am J Pharm Heal Res 2015;3:1–8.
Pappachen LK, Chacko A. In vitro antioxidant activity and determination of total phenolic, flavonoid contents of peperomia pellucida linn. Am J Pharm Heal Res 2013;1:99–101.
Noreen H, Semmar N, Farman M, McCullagh JSO. Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant coronopus didymus. Asian Pac J Trop Med 2017;10:792–801.
Sp D, Raj BA, Muruganantham N, Tk P, Ps R. Screening of wrightia tinctoria leaves for antipsoriatic activity screening of wrightia tinctoria leaves for antipsoriatic activity. J Drugs Med 2012;4:73–8.
Lestari MW, Fidrianny I, Yusuf AT, Soemardji AA. Brine shrimp teratogenic screening test on some selected plants. Asian J Pharm Clin Res 2017;10:96–9.
Sreejamole KL, Greeshma PM. Antioxidant and brine shrimp cytotoxic activities of ethanolic extract of red alga Cracilaria corticata. J Agardh Indian J Nat Prod Resour 2013;4:233–7.
Arung ET, Wicaksono BD, Handoko YA, Kusuma IW, Yulia D, Sandra F. Anti-cancer properties of diethylether extract of wood from Sukun (Artocarpus altilis) in human breast cancer (T47D) cells. Trop J Pharm Res 2009;8:317–24.
Zarei O, Yaghoobi MM. Cytotoxic and anti-proliferative effects of Rosa beggeriana Schrenk extracts on human liver and breast cancer cells. Avicenna J Phytomed 2019;9:386–95.
Khan F, Ahmed F, Pushparaj PN, Abuzenadah A. Ajwa date (Phoenix dactylifera L.) extract inhibits human breast adenocarcinoma (MCF7) cells in vitro by inducing apoptosis and cell cycle arrest. PLoS One 2016;11:1–17.
Umesh Khandekar, Anil Bobade RG. Evaluation of antioxidant activity, in vitro atimicrobial activity and phytoconstituents of schleichera oleosa (lour.) oken. Int J Biol Pharm Res. 2015;6:137–43.
SD Sanja, Sheth NR, Patel NK, Patel D, Patel B. Characterization and evaluation of the antioxidant activity of portulaca oleracea. Int J Pharm Pharm Sci 2009;1:74–84.
Baskar R, Rajeswari V, Kumar TS. In vitro antioxidant studies in leaves of annona species. Indian J Exp Biol 2007;45:480–5.
Chanda S, Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: an overview. Afr J Microbiol Res 2009;3:981–96.
Ck R, Kl R, Ks J, Joyce GS. Comparative evaluation of antioxidant property in methanol extracts of some common vegetables of India. Sch Res Libr 2011;2:86–94.
Krishnaraju AV, Rao TVN, Sundararaju D, Vanisree M. Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) Lethality Assay †. Int J Appl Sci Eng 2005;3:125–34.
Muruganandam LRM. Anticancer studies of selective mannich bases by in silico method. Int J Curr Pharm Res 2018;10:81.
Published
How to Cite
Issue
Section
