EXTRACTION, ISOLATION OF ACTIVE PRINCIPLES, ANTI-BACTERIAL AND WOUND HEALING ACTIVITY OF THE MARINE ALGAL SPECIES OEDOGONIUM GLOBOSUM AND OEDOGONIUM INTERMEDIUM
Keywords:Oedogonium Globosum, Oedogonium Intermedium, Extraction, Phytochemical analysis, Isolation, Dermal irritation test, Anti-microbial and Wound healing Activities
Objective: Algae is the undisputed treasures of the sea and are a valuable raw material, providing unlimited opportunities for new drug discoveries. Marine algal products are in demand in the international market in the form of standardized algal extracts or semi-finished products.
Methods: Aqueous and methanolic extracts of Oedogonium globosum and Oedogonium intermedium species were obtained maceration and hot percolation. The active principles from O. intermedium were isolated, purified by column chromatography, and characterized by spectral studies IR, λmax, 1HNMR and MS. The extracts of Oedogonium species were screened for their anti-microbial effects, acute dermal irritation and wound-healing activity studies.
Results: Comparing to Oedogonium intermedium (45 %, 90 %, 87 %), very low extractive yields were obtained for Oedogonium globosum (10.80 %, 37 %, 28 %). At phytochemical screening, Terpenoids, Flavanoids and, Glycans were found to be present in a significant amount and upon their isolation, it was found that a collection of fractions from cold extract with Rf value in the range 0.32-0.34 as Glycans and those from the hot extract with 0.40-0.72 as Flavanoids and those from methanolic extracts with 0.23, 0.44 and 0.71 as for Terpenoids. Anti-bacterial study revealed out the fact of Oedogonium species could give higher inhibition to gram-positive than for gram-negative bacteria at (10 μg/10μl/disc) concentration. No symptoms of systemic toxicity and mortality were observed. Silver sulfadiazine, more potent in wound closure, the effect of methanolic extracts of O. intermedium (87 %) was almost at par to the standard (95 %) in action and significantly greater than O. globosum (72 %, P<0.05).
Conclusion: Admittedly, Oedogonium type algal species can be known as medicinal algae with a plethora of a wide range of pharmacological activities. Thus, this research work may be considered further for extensive innovative discoveries of new lead molecules and any other pharmacological activities, in the future.
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