• HARSAHAY MEENA Defence Institute of Bio-Energy Research (DIBER) DRDO, Goraparao, Haldwani 263139, Dist. Nainital, Uttarakhand (India)
  • V. K. JOSHI Defence Institute of Bio-Energy Research (DIBER) DRDO, Goraparao, Haldwani 263139, Dist. Nainital, Uttarakhand (India)
  • MADHU BALA Defence Institute of Bio-Energy Research (DIBER) DRDO, Goraparao, Haldwani 263139, Dist. Nainital, Uttarakhand (India)




Adrenergic, Antidepressant, Dopaminergic, Serotonergic Systems and V wallichii


Objective: Valeriana (V) wallichii DC is found among the ground flora of Himalayan regions and used as herbal medicine for CNS disorders in Indian systems of medicine. In the study, aimed to investigation of involvement of neuro-chemical-systems in V. wallichii induced antidepressant effects in mice.

Methods: The antidepressant activity of aqueous alcoholic extracts of V. wallichii was measured by using Forced Swim Test and Tail Suspension Test in mice, whereas locomotor activity was observed by Actophotometer. Involvement of adrenergic, dopaminergic and serotonergic receptors in V. wallichii induced antidepressant effects in mice were also observed.

Results: Sub-acute administration of V. wallichii in mice showed significant (P<0.05) increase in the antidepressant activity similar to the Imipramine, but did not show at lower doses in FST. Whereas, pretreatment of adrenergic receptor antagonist, dopaminergic receptor antagonist and serotonin synthesis inhibitor prevented V. wallichii induce anti-depressant effect in mice. Lower doses of hydoalcoholic extract of V. wallichii did not interfering the locomotor activity in mice during the sub-acute administration, but at a higher dose significantly (P<0.05) decreases the locomotor activity.

Conclusion: Hence, it is concluded that the antidepressant effect of V. wallichii in mice may mediate via adrenergic, dopaminergic and serotonergic systems.


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Nadkarni KM. Indian materia medica. 3rd ed. Bombay, Popular Prakashan; 1976. p. 260.

Sharma PV. Priya Nighuntu. 1st ed. Varanasi, Chaukhambha Surabharati Prakasan; 2001. p. 60.

Becker H, Chavadej S. Valepotriates: production by plant cell cultures. In biotechnology in agriculture and forestry, edited by Bajaj YPS. Medicinal and Aromatic Plants. Berlin Springer Verlag 1988;4:294-309.

Said HM. Hamdard pharmacopoeia of eastern medicine. Karachi, Times Press; 1970.

Marder M, Viola H, Wasowski C, Fernandez S, Medina JH, Paladini AC. 6-methylapigenin and hesperidin: new Valeriana flavonoids with activity on the CNS. Pharmacol Biochem Behav 2003;75:537-45.

Sharma R. Medicinal plants of India. In: An encyclopedia. New Delhi, Daya Publishing House; 2003.

Gilani AH, Khan AU, Jabeen Q, Subhan F, Ghafar R. Antispasmodic, and blood pressure lowering effects of Valeriana wallichii are mediated through K+channel activation. J Ethnopharmacol 2005;100:347-52.

Rao SN, Mittal S, Menghani E. Evaluation of antioxidant properties of Valeriana Wallichi to scavenge free radicals. Asian J Pharm Clin Res 2012;5:238-40.

Fernandez S, Wasowski C, Paladini AC, Marder M. Sedative and sleep-enhancing properties of linarin, a flavonoid-isolated from Valeriana officinalis. Pharmacol Biochem Behav 2004;77:399-404.

Bhattacharyya D, Jana U, Debnath PK, Suri TK. Initial exploratory observational pharmacology of Valeriana wallichii on stress management: a clinical report. Nepal Medical College J 2007;9:36-9.

Rehni AK, Pantlya HS, Shri R, Singh M. Effect of chlorophyll and aqueous extracts of Bacopa monniera and Valeriana wallichii on ischemia and reperfusion induced cerebral injury in mice. Ind J Exp Biol 2007;45:764-9.

Shalam M, Shantakumar SM, Narasu ML. Pharmacological, and biochemical evidence for the antidepressant effect of the herbal preparation Trans-01. Ind J Pharmacol 2007;39:231-4.

Nandhini S, Narayan KB, Ilango K. Valeriana officinalis: a review of its traditional uses, phytochemistry and pharmacology. Asian J Pharm Clin Res 2018;11:36-41.

Subhan F, Karim N, Gilani AH, Sewell RDE. Terpenoid content of Valeriana wallichii extracts and antidepressant-like response profiles. Phytother Res 2009;24:686-91.

Thies PW. Linarin-isovalerianate, a currently unknown flavonoid from Valeriana wallichii. Planta Med 1968;16:363-71.

Bounthanh C, Bergmann C, Beck JP, Hagg Berrurier M, Anton R. Valpotriates, a new class of cytotoxic and antitumor agents. Planta Med 1981;41:21-8.

Willis RB, Bone K, Morgan M. Herbal products: active constituents, mode of action and quality control. Nutr Res Rev 2000;13:47-77.

Organization for Economic Cooperation and Development (OECD) Guidelines for Testing of Chemicals. Guidelines 423, Acute Oral Toxicity-Acute Toxic Class Method; 1996.

Porsolt RD, Bertin A, Jalfre M. Behaviour despair in mice: a primary screening test for antidepressant. Archives Internationes De Pharmacodynamie Et De Therapie 1977;229:327-36.

Brocardo PS, Budni J, Lobato KR, Kaster MP, Rodrigues AL. Antidepressant like effect of folic acid: involvement of NMDA receptors and L-arginine–nitric oxide–cyclic guanosine monophosphate pathway. Eur J Pharmacol 2008;598:37-42.

Rosa AO, Kaster MP, Binfare RW, Morales S, Martin Aparicio E, Navarro Rico ML et al. Antidepressant-like effect of the novel thiadiazolidinone NP031115 in mice. Progr Neuropsychopharmacol Biol Psychiatry 2008;32:1549-56.

Wesołowska A, Nikiforuk A, Stachowicz K, Tatarczyńska E. Effect of the selective 5-HT7 receptor antagonist SB 269970 in animal models of anxiety and depression. Neuropharmacology 2006;51:578–86.

Steru L, Chermat R, Thierry B, Simon P. The tail suspension test: a new method for screening antidepressants in mice. Psychopharmacol 1985;85:367-70.

Reddy DS, Kaur G, Kulkarni SK. Sigma (sigma1) receptor mediated antidepressant-like effects of neurosteroids in the porsolt swim test. Neuroreports 1998;9:3069-73.

Koh JH, Kim KM, Kim JM, Song JC, Suh HJ. Antifatigue and antistress effect of the hot-water fraction from mycelia of Cordyceps sinensis. Biol Pharmacol Bull 2003;26:691-4.

Nozawa Y, Yamada K, Okabe Y, Ishizaki T, Kuroda M. The antifatigue effects of the low-molecular-weight fraction of bonito extract in mice. Biol Pharmacol Bull 2009;32:468-74.

Detke MJ, Rickels M, Lucki I. Active behavior in the rat forced swimming test differentially produced by serotonergic and noradrenergic antidepressant. Psychopharmacology 1995;121: 66-72.

Whillner P. The validity of animal model of depression. Psychopharmacology 1984;83:1-16.

Subhan F, Karim N, Gilani AH, Sewell RD. Terpenoid content of Valeriana wallichii extracts and antidepressant-like response profiles. Phytother Res 2010;24:686-91.

Ahmad G, Amin KM, Khan NA, Tajuddin M. The anti-stress activity of a gem-containing Unani formulation against diverse stressors. J Ethnopharmacol 1998;59:187-93.

Sah SP, Mathela CS, Chopra K. Antidepressant effect of Valeriana wallichii patchouli alcohol chemotype in mice: behavioural and biochemical evidence. J Ethnopharmacol 2011;135:197-200.



How to Cite

MEENA, H., V. K. JOSHI, and M. BALA. “EVALUATION OF INVOLVEMENT OF NEURO-CHEMICAL MECHANISM IN VALERIANA WALLICHII INDUCED ANTIDEPRESSANT EFFECT IN MICE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 13, no. 5, May 2021, pp. 11-17, doi:10.22159/ijpps.2021v13i5.40615.



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