SCREENING OF ANTIBIOFILM AND ANTI-QUORUM SENSING POTENTIAL OF VITEX TRIFOLIA IN PSEUDOMONAS AERUGINOSA
Keywords:
Vitex trifolia, Quorum sensing inhibition, Acyl homoserine lactone, Pseudomonas aeruginosa, Antibacterial assay, AntibiofilmAbstract
Objective: In this present study the methanolic leaf extract of Vitex trifolia was assessed for its anti quorum sensing activity against acyl-homoserine lactone (AHL)-dependent virulence factors in Pseudomonas aeruginosa.
Methods: In this study the methanolic leaf extract of Vitex trifolia was screened to control the virulence factors of Pseudomonas aeruginosa by antibacterial analysis, biofilm prevention assay, protease and elastase assay, pyocyanin production, EPS (Exopolysaccaride) assay, Swimming assay and Light microscopic analysis.
Results: The methanolic leaf extract obtained from Vitex trifolia exhibited a concentration dependent (50-100 μg/ml) reduction in anti quorum sensing activity in Pseudomonas aeruginosa. The extract inhibited 69% of protease activity, 48% of elastase activity and 96% of exopolysaccharide production in Pseudomonas aeruginosa. The pigment pyocyanin production and swimming motility of the bacteria were also controlled.
Conclusion: Thus in this study the methanolic leaf extract of Vitex trifolia was found to be potent against anti quorum sensing activity in Pseudomonas aeruginosa.
Â
Downloads
References
Annapoorani A, Jabar AKKA, Musthafa SK, Pandian SK, Ravi AV. Inhibition of Quorum sensing mediated virulence factors production in urinary pathogen Serratia marcescens PS1 by Marine sponges. Indian J Microbiol 2012;52:160-6.
Hejazi A, Falkiner FR. Serratia marcescens. J Med Microbiol 1997;46:903-12.
Khadar SM, Krishnaswamy B, Shunmugaiah KP, Arumugam VR. 2, 5-Piperazinedione inhibits quorum sensing–dependent factor in Pseudomonas aeruginosa. J Basic Microbiol 2012;52:1-8.
Kumar RJ, Zi-rong X. Biomedical compounds from marine organisms. Mar Drugs. 2004;2:123-46.
Nancy IMR, Meenashree B, Vasanthi J. Screening of antibacterial and qualitative and quantitative analysis of phytochemicals in vitex trifolia. Int J Curr Microbiol Appl Sci 2014;3(5):425-31.
Nancy IMR, Banu N. Inhibition of Antibiofilm mediated virulence factors production in urinary pathogen Serratia marcescens by Vitex trifolia. Int J Sci Res 2014;3(8):1972-4.
Packiavathy IASV, Agilandeswari P, Musthafa KS. Pandian SK, Ravi AV. Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against gram negative bacterial pathogens. Food Res Int 2012;45:85-92.
Packiavathy IASV, Agilandeswari P, Ramaswamy RP, Pandian SK, Ravi AV. Antiquorum sensing and antibiofilm potential of Capparis spinosa. Arch Med Res 2011;45:85-92.
Rice SA, Koh KS, Queck SY, Labbate M, Lam KW, Kjelleberg S. Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues. J Bacteriol 2005;187:477-85.
Sarah JC, Neil RW, Abigail KPH, David RS, George PCS. Metabolic and regulatory engineering of Serratia marcescens mimicking phage-mediated horizontal acquisition of antibiotic biosynthesis and quorum-sensing capacities. Microbiology 2006;152:899-911.
Skindersoe M, Epstein EP, Rasmussen TB, Bjarnsholt T, de Rocky Nys, Givskov M. Quorum sensing antagonism from marine organisms. Mar Biotechnol 2008;10:56-3.
Thenmozhi R, Nithyanand P, Rathna J, Pandian SK. Antibiofilm activity of coral-associated bacteria against different clinical M serotypes of Streptococcus pyogenes. FEMS Immunol Med Microbiol 2009;57:284-94.
Traub WH. Antibiotic susceptibility of Serratia marcescens and Serratia liquefaciens. Chemotherapy 2000;46:315-22.