• Senthamarai Kannan Balakrishnan Supramolecular Research Laboratory, Department of Chemistry, Loyola College, Chennai-600034, India.
  • Suresh Kumar Dass Supramolecular Research Laboratory, Department of Chemistry, Loyola College, Chennai-600034, India.
  • Host Antony David Rajendran Department of Molecular Biology, Entomology Research Institute, Loyola College, Chennai-600034, India.
  • Ignacimuthu Savrimuthu Department of Molecular Biology, Entomology Research Institute, Loyola College, Chennai-600034, India.


HepG2 cancer cells, Cytotoxicity, MTT assay, Antibacterial activity, Antifungal activity, Ruthenium polypyridyl complexes


Objectives: To evaluate the in vitro cytotoxicity, antibacterial and antifungal activity of the synthesized mono-, di- and trinuclear ruthenium(II) polypyridine complexes.

Methods: A series of synthesized ruthenium(II) complexes, R1, R2 and R3, are screened for in vitro antiproliferative activity against HepG2 cancer cell line using 96-well plate method. An assay of antimicrobial activity was performed by disc diffusion method. In addition an assay of an antifungal was performed by broth micro-dilution method.

Results: The cytotoxicity of complexes revealed IC50 values of 14.52 (R3), 19.53 (R2) and 22.32 μM (R1) against HepG2 cell line in a dose dependent manner. All the complexes inhibited moderately the growth of Gram positive bacteria (G+) such as Staphylococcus aureus (MRSA), Eubacterium lentum, and Bacillus subtillis, quite meagerly the growth of the Gram negative bacterium (G─), Enterobacter aerogenes, but did not inhibit at all the growth of Erwinia amylovora (MTCC 2760) and showed a slight antifungal activity.

Conclusion: From this study, we could suggest that the systematic increase in number of imidazole moiety along with expanding cloud of conjugated π-electron system of ruthenium(II) polypyridine complexes is responsible for the antiproliferative activity which increases in the order, R1 < R2 < R3 against HepG2 cancer cells. Consecutively, the complexes show good antimicrobial activity against Gram positive bacteria, but show poor or no effect against Gram negative bacteria and exhibit a little antifungal activity.



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How to Cite

Balakrishnan, S. K., S. K. Dass, H. A. D. Rajendran, and I. Savrimuthu. “CYTOTOXICITY AND ANTIMICROBIAL ACTIVITY OF MONO-, DI- AND TRINUCLEAR RUTHENIUM(II) POLYPYRIDINE COMPLEXES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 317-21,



Original Article(s)