• Nayan Pradhan Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Majhitar 737132, East Sikkim, India
  • Hiyashree Rajkhowa Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Majhitar 737132, East Sikkim, India
  • Hemant Giri Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Majhitar 737132, East Sikkim, India
  • Bhupendra Shrestha Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Majhitar 737132, East Sikkim, India


Moxifloxacin hydrochloride, Doxorubicin hydrochloride, Simultaneous estimation, Method development, Validation


Objective: To develop a simple, accurate, and precise spectrophotometric method for the simultaneous estimation of moxifloxacin hydrochloride (MOX) and doxorubicin hydrochloride (DXR).

Methods: MOX and DXR solution were simultaneously determined in 0.1M HCl at their respective λmax. The absorbance λmax of MOX and DXR was 295 nm and 480 nm, respectively. The developed method was validated according to ICH guidelines for parameters like linearity, accuracy, precision, ruggedness and robustness.

Results: Molar absorptivities of MOX and DXR were found to be more in 0.1M HCl with compared to water, methanol and 0.1M NaOH. Linearity was obtained over the range 0.5–20.0 μg/ml and 1.5-50 μg/ml with a lower limit of quantitation of 0.25 μg/ml and 1.5 μg/ml for MOX and DXR, respectively. For each level of samples, inter-and intra-day precision ( % RSD) was<1.3 % and<1.4 % for MOX and<2.5 and 2.4 % for DXR, respectively. The mean recovery of MOX and DXR was in the range 96.21 %-98.77 % and 97.13 %-99.64 %, respectively.

Conclusion: The method developed was validated as per ICH guidelines for parameters like linearity, accuracy, method precision, robustness and ruggedness. The results obtained were well within the acceptable criteria. The method can be used for routine analysis of MOX and DXR.


Download data is not yet available.

References [Last accessed on 24 Sep 2014]

Hauser AR. Antibiotic basics for clinicians: choosing the right antibacterial agent: Lippincott Williams and Wilkins; 2007.

Stewart J, Warren F, King D, Venkateshwaran T, Ponder G, Fox J. Stability of ondansetron hydrochloride, doxorubicin hydrochloride, and dacarbazine or vincristine sulfate in elastomeric portable infusion devices and polyvinyl chloride bags. Am J Health Syst Pharm 1997;54:915-20.

The Indian Pharmacopoeia Commission Ghaziabad. Indian Pharmacopeia; 2007. p. 441.

Trevor A, Katzung B, Masters S. Pharmacology: Examination and Board Review. Chapter 54, Anthracycilne Antibiotics; 2012. Available from: URL: [Last accessed on 25 Jan 2013]

Fujiwara A, Hoshino T, Westley JW. Anthracycline antibiotics. Crit Rev Biotech 1985;3:133.

Cutler SJ. Biologically active natural products: pharmaceuticals. CRC Press; 2000. p. 5.

Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 y. J Nat Prod 2007;70:461-77.

Richards AD, Rodgers A. Synthetic metallomolecules as agents for the control of DNA structure. Chem Soc Rev 2007;36:471–83.

Johansson S, Goldenberg D, Griffiths G. Elimination of HIV-1 infection by treatment with a doxorubicin-conjugated anti-envelope antibody. AIDS 2006;20:1911–5.

Pigram WJ, Fuller W, Hamilton LD. Stereochemistry of intercalation: interaction of daunomycin with DNA. Nature New Biol 1972;235:17–9.

Facts and Statistics. The Leukemia and Lymphoma Society; 2009.

Reuveni D, Halperin D, Shalit I, Priel E, Fabian I. Quinolones as enhancers of camptothecin-induced cytotoxic and anti-topoisomerase I effects. Biochem Pharmacol 2008;75:1272–81.

Reuveni D, Halperin D, Fabian I, Tsarfaty G, Askenasy N, Shalit I. Moxifloxacin increases anti-tumor and anti-angiogenic activity of irinotecan in human xenograft tumors. Biochem Pharmacol 2010;79:1100–7.

Barot M, Gokulgandhi MR, Pal D, Mitra AK. In vitro moxifloxacin drug interaction with chemotherapy: Implications for retinoblastoma management. Exp Eye Res 2014;118:61-71.

Lai ECS, Lo CM, Fan ST, Liu CL, Wong J. Postoperative adjuvant chemotherapy after curative resection of hepatocellular carcinoma: a randomized controlled trial. Arch Surg 1998:133:183-8.

Zoli W, Ricotti L, Tesei A, Barzanti F, Amadori D. In vitro preclinical models for a rational design of chemotherapy combinations in human tumors. Crit Rev Oncol Hematol 2001;37:69-82. [Last accessed on 24 Sep 2014] Last accessed on 24 Sep 2014]

Motwani SK, Chopra S, Ahmad FJ, Khar RK. Validated spectrophotometric methods for the estimation of moxifloxacin in bulk and pharmaceutical formulations. Spectrochim Acta Part A 2007;68:250-6.

Kaur K, Kumar A, Malik AK, Singh B, Rao A. Spectrophotometric methods for the determination of fluoroquinolones: a review. Crit Rev Anal Chem 2008;38:2-18.

Sturgeon RJ, Schulman SG. Electronic absorption spectra and protolytic equilibria of doxorubicin: direct spectrophotometric determination of microconstants. J Pharm Sci 1977;66:958-61.

Patel RK, Parmar RR, Patel VM, Shah DA. Method development and validation of cefixime and moxifloxacin in pharmaceutical dosage form by UV spectrophotometric method. Int J Pharm Res Bio-Sci 2012;1:81-93.

Vyas P, Dave J, Patel C. Simultaneous estimation of Moxifloxacin Hcl and Bromfenac sodium in eye drops by spectrophotometric methods. Int J Pharmal Sci Res 2012;3:2137-42.

Abdellaziz LM, Hosny MM. Development and validation of spectrophotometric, atomic absorption and kinetic methods for determination of moxifloxacin hydrochloride. Anal Chem Insights 2011;6:67.

Sahu S, Azam MA, Sahu D, Banarjee M. Spectrophotometric estimation of moxifloxacin in bulk and its pharmaceutical formulations. Pharmacologyonline 2011;2:491-502.

Mishra M. Simple and validated UV-spectroscopic method for estimation of moxifloxacin. HCL in bulk and formulation. J Global Pharm Technol 2010;2:21-7.

Chintawar PP, Pawar PN, Harde MT, Joshi SV, Chaudhari PD. Spectrophotometric methods for simultaneous estimation of moxifloxacin HCl and ketorolac tromethamine. Asian J Res Chem 2010;3:767-71.

Srinivas N, Narasu L, Shankar BP, Mullangi R. Development and validation of a HPLC method for simultaneous quantitation of gatifloxacin, sparfloxacin and moxifloxacin using levofloxacin as internal standard in human plasma: application to a clinical pharmacokinetic study. Biomed Chromatogr 2008;22:1288-95.

Baietto L, D'Avolio A, De Rosa FG, Garazzino S, Patanella S, Siccardi M, et al. Simultaneous quantification of linezolid, rifampicin, levofloxacin, and moxifloxacin in human plasma using high-performance liquid chromatography with UV. Ther Drug Monit 2009;31:104-9.

Kamruzzaman M, Alam A-M, Lee SH, Suh YS, Kim YH, Kim GM, et al. Method for determination of fluoroquinolones based on the plasmonic interaction between their fluorescent terbium complexes and silver nanoparticles. Microchim Acta 2011;174:353-60.

Sultana N, Akhtar M, Shamim S, Gul S, Arayne MS. Simultaneous determination of moxifloxacin and H2 receptor antagonist in pharmaceutical dosage formulations by RP-HPLC: application to in vitro drug interactions. Quim Nova 2011;34:683-8.

Dewani A, Barik B, Kanungo S, Wattyani B, Chandewar A. Development and validation of RP-HPLC method for the determination of moxifloxacin in presence of its degradation products. Am Eurasian J Sci Res 2011;6:192-200.

Devika G, Sudhakar M, Rao JV. Simultaneous estimation of cefixime and moxifloxacin in bulk and its pharmaceutical dosage form by RP-HPLC. Orient J Chem 2012;28:1743-50.

Sousa J, Alves G, Fortuna A, Falcão A. Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review. Anal Bioanal Chem 2012;403:93-129.

Barth HG, Conner AZ. Determination of doxorubicin hydrochloride in pharmaceutical preparations using high-pressure liquid chromatography. J Chromatogr A 1977;131:375-81.

Sastry CS, Lingeswara Rao JS. Determination of doxorubicin hydrochloride by visible spectrophotometry. Talanta 1996;43:1827-35.

Shinozawa S, Oda T. Determination of adriamycin (doxorubicin) and related fluorescent compounds in rat lymph and gall by high-performance liquid chromatography. J Chromatogr A 1981;212:323-30.

Dubois J, Hanocq M, Atassi G. Determination of doxorubicin, Daunorubicin and some of their metabolites in mouse plasma by high-performance reversed-phase liquid chromatography with amperometric detection. Anal Lett 1987;20:1611-34.

King D, Stewart J. HPLC determination of dacarbazine, doxorubicin, and ondansetron mixture in 5 % dextrose injection on underivatized silica with an aqueous-organic mobile phase. J Liq Chromatogr Relat Technol 1993;16:2309-23.

King D, Venkateshwaran T, Stewart JT. HPLC determination of a vincristine, doxorubicin, and ondansetron mixture in 0.9 % sodium chloride injection. J Liq Chromatogr Relat Technol 1994;17:1399-411.

Fogli S, Danesi R, Innocenti F, Di Paolo A, Bocci G, Barbara C, et al. An improved HPLC method for therapeutic drug monitoring of daunorubicin, idarubicin, doxorubicin, epirubicin, and their 13-dihydro metabolites in human plasma. Ther Drug Monit 1999;21:367.

Gianni L, Munzone E, Capri G, Fulfaro F, Tarenzi E, Villani F, et al. Paclitaxel by 3h infusion in combination with bolus doxorubicin in women with untreated metastatic breast cancer: high antitumor efficacy and cardiac effects in a dose-finding and sequence-finding study. J Cereals Oilseeds 1995;13:2688-99.

Cullen M. Antibacterial prophylaxis after chemotherapy for solid tumors and lymphomas. N Engl J Med 2005;353:988-98.

Lebrecht D, Walker UA. Role of mtDNA lesions in anthracycline cardiotoxicity. Cardiovasc Toxicol 2007;7:108-13.



How to Cite

Pradhan, N., H. Rajkhowa, H. Giri, and B. Shrestha. “SIMULTANEOUS SPECTROPHOTOMETRIC ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DOXORUBICIN HYDROCHLORIDE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, Nov. 2015, pp. 21-26,



Original Article(s)