METHOD DEVELOPMENT FOR SIMULTANEOUS ANALYSIS OF MARKER SCOPOLETINE, ANDROGRAPHOLIDE, QUERCETIN, AND LUTEOLIN IN ANTIHYPERTENSION JAMU FORMULATION USING RP-HPLC
Keywords:
Antihypertension jamu formulation, Scopoletine, Andrographolide, Quercetin, Luteolin, HPLCAbstract
Objective: The aim of this research was to develop a method of simultaneous analysis marker compounds in antihypertension jamu formulation using reverse phase high performance liquid chromatography (RP-HPLC).
Methods: Stationary phase, mobile phase, sample preparation and instrument condition were optimized.
Results: Analysis of marker compounds in anti hypertension jamu formulation using HPLC with reverse phase ODS C18 column (LiChroCART®, 125 x 4.6 mm, particle size of 5 µm) and gradient mobile phase of formic acid 1% and methanol could separate markers in 35 min at flow rate 2 mL/min. Four markers in antihypertension jamu formulation have been successfully analyzed simultaneously, were scopoletine, andrographolide, quercetin, and luteolin. The RSD values demonstrated the intra and inter-day precisions were less than 4% and 6.5% respectively and the recoveries around 93.45-114.42%, respectively. All calibration curves showed good linear correlation coefficient which of R > 0.995.
Conclusion: RP-HPLC method has been developed for simultaneous analysis of four markers in antihypertension jamu. The methods can be used to analyze antihypertension jamu formulation in the market.
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References
Patel RK, Patel VR, Patel MG. Development and validation of a RP-HPLC method for the simultaneous determination of embelin, rottlerin and ellagic acid in vidangadi churna. J Pharm Anal 2012;2(5):366-71.
Shams G. Development of HPLC fingerprint analysis of traditional diabetes herbal jamu diabetes plants materials. J Teknologi (Sci Eng) 2014;68(1):83-8.
Zheng L, Dong D. Development and validation of an HPLC method for simultaneous determination of nine active components in da-chai-hu-tang. Chin Med 2011;2(1):20-8.
Wang GL, Wei M, Wang J, Lu Y, Mahady GB, Liu D. High-performance liquid chromatography with photodiode array (HPLC-PDA) quality control of menoprogen, a traditional Chinese medicine (TCM) formula used for the management of menopause. Int J Med Plants Res 2013;2(1):146-51.
Kemenkes: Farmakope herbal indonesia. 1st ed. Jakarta, Kemenkes; 2009.
Ahmed MF, Rao AS. HPLC simultaneous determination of phenolic compounds in brassica olerocea l. var capitata by high-performance liquid chromatography. Int J Pharm Sci 2014;6(1):534-7.
Subramanian G, Meyyanathan SN, Karthik Y, Karunakaranair A, Palanisamy DS. Development and validation of HPLC method for the simultaneous estimation of quercetin and rutin in aganosma dichotoma (Roth) K. Schum. Int J Phar Sci 2014;6(1):606-8.
Yang L, Yan QH, Ma JY, Wang Q, Zhang JW, Xi GX. High performance liquid chromatographic determination of phenolic compounds in propolis. Trop J Pharm Res 2013;12(5):771.
Li J, Jiang B, Liu X, Zhang J, Chen X, Bi K. Simultaneous determination of five bioactive flavonoids in Hypericum japonicum thunb by high-performance liquid chromatography. Asian J Tradit Med 2007;2(2):75-81.
Aryani T. Pengujian validasi analisis kadar andrografolid secara kromatografi cair kinerja tinggi (KCKT) dengan eluasi gradien terhadap ekstrak herba sambiloto (Andrographis paniculata Ness). Berkas Penelitian Hayati 2005;11:73–6.
West BJ, Deng S. Thin layer chromatography methods for rapid identity testing of morinda citrifolia l. (Noni) fruit and leaf. Adv J Food Sci Technol 2010;2(5):298-302.
International conference on harmonization: Validation of analytical procedures: Text and Methodology Q2(R1); 1995. p. 3,7-13.
AOAC: Guidelines for single laboratory validation of chemical methods for dietary supplements and botanicals; 2002.
FDA: Reviewer guidance validation of chromatographic methods; 1994.
Health sciences authority: ASEAN guildelines for validation analytical Prosedurer; 2012.
Wisnuwardhani HA, Fidrianny I, Ibrahim S. Method development for simultaneous analysis of steroid and non steroid antiinflamatory substances in jamu pegal linu using TLC spectrophotodensitometri. Int J Pharm Pharm Sci 2013;5(4):749-53.