THE EFFECTS OF HOMOCYSTEINE ON PLASMA BIOCHEMICAL PARAMETERS AND AORTIC MATRIX METALLOPROTEINASES ACTIVITIES
Keywords:
Methionine, Homocysteine, Cholesterol, Biochemical parameters, MMPs, Extracellular matrixAbstract
Objective; Increased levels of physiological amino acid homocysteine (Hcy) in plasma is associated with the development of cardiovascular, neuronal, and liver diseases.[1] Damage of the vascular wall of aorta develops by exposure of vessel to the not yet degraded, toxically acting hcy, as occurs in the beginning of arteriosclerosis.[2]
Methods; Our investigation was performed in two parts on 12 male wistar rats, 3-4 months old in both. First we investigated the relation between homocysteine and other plasma biochemical parameters which is related with cardiovascular events: Total cholesterol (CT), HDL cholesterol (HDL-C), LDL cholesterol (LDL-C), triglyceride (TG). We employed 3 groups: (C) control. (Met) rats received methionine in high doses (200mg/Kg/day), (Met-C) in order to potentiate the atherogenic effect cholesterol was administred (1500mg/Kg/day). Lipid parameters were measured, and structural damage to an aorta was analyzed by histology.
In the second part, we used 3 groups: (C) control. (M) methionine (1g/Kg/day), (H) Hcy (20mg/day) to compare the effects of both methionine and homocysteine on aortic MMPs expression.
Results & Conclusion: The results show that elevated plasma homocysteine increase cholesterol synthesis, exerts an angiotoxic action direct to aorta (loss of endothelium, degeneration partly with dissolution of media cells), and induce expression of MMP-2, while MMP-9 was not expressed.
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References
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