IN-VIVO ANTI-PYRETIC, ANTI-NOCICEPTIVE, NEUROPHARMACOLOGICAL ACTIVITIES AND ACUTE TOXICITY INVESTIGATIONS OF BLUMEA LACERA
Keywords:
Blumea lacera, In-vivo antipyretic activities, Anti-nociceptive activity, Neuropharmacological activities, Acute toxicityAbstract
Objective: The present study was done to evaluate in vivo anti-pyretic, neuropharmacological activity including open field and swimming test, gastrointestinal motility, anti-nociceptive and acute toxicity effect of different leaf extracts of Blumea lacera in Swiss albino mice following oral administration.
Methods: In-vivo antipyretic test of methanol, ethanol and chloroform extracts of Blumea lacera leaf was done brewer's yeast method; neuropharmacological study was performed by open field test and swimming test, GI motility test was done by charcoal induced anti motility test, anti-nociceptive activity was tested by acetic acid induced writhing method and acute toxicity study was done by investigating mortality/morbidity status of test animal.
Results: In-vivo antipyretic activity shows methanol extracts at a dose of 400 mg/kg b. w., both the doses of ethanol extract and chloroform extract at a dose of 200 mg/kg b. w. produced significant (p<0.05) reduction of temperature in mice comparing to the standard drug diclofenac Na. In-vivo neuropharmacological activity study yields significant results when methanol 100 mg/kg, ethanol 200 mg/kg, chloroform 100 & 200 mg/kg extracts were administered to evaluate the rate of movement with time in a dose dependent manner when compared with the corresponding value of control group. In gastrointestinal motility test methanol extract at 250 mg/kg, ethanol extract at 250 & 500 mg/kg and chloroform 250 mg/kg doses significantly reduce GI motility when compared to standard drug loperamide. Statistically significant (p <0.001, p<0.02, p<0.05) results were found in in-vivo anti-nociceptive activity test for the 100 mg/kg chloroform, 100 mg/kg methanol and 200 mg/kg ethanol respectively when compared to standard diclofenac-Na. None of the extracts showed any significant in-vivo acute toxicity effect on mice.
Conclusion: This plants leaf extracts exhibit potent antipyretic acitivities; significant neuropharmacological activities and significant anti-nociceptive activity without inducing any discernible acute toxicity effect.
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