NEUROPROTECTIVE EFFECTS OF PIMPINELLA ANISUM ON NORMAL AND DIABEITC RATS
Keywords:
Bisphinol A, Streptozetocin, Pimpinella anisum, NeurotoxicityAbstract
Objective: Bisphenol A (BPA) was a polycarbonate plastic used in plastic containers, baby's bottle, water bottles, laptops, mobiles, and food canes, etc. It is associated with obesity and insulin resistance. The food and Drug Administration (FAD), The Environmental Protection Agency (EPA) and the Occupational Safety and Health Administration (OSHA) recommended more study on this material. Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. Diabetes can cause many complications like cardiovascular risk, chronic renal failure, nephropathy etc. The present study was done to know how to decrease the toxic effect of BPA by Pimpinella anisum (Anise) on the physiological and neurological parameter on normal and diabetic rats induced by Streptozetocin (STZ).
Methods: Rats involved in this study were divided into eight groups: 1st control group and received citrate buffer, 2nd diabetic group treated with STZ (50mg/kg. b. wt), 3rd BPA group treated with BPA (30mg/kgbwt), 4th Anise group treated with anise oil. (3g/kgb. wt),5th STZ + BPA group combination between STZ and BPA with the same dose, 6th group treated with (STZ+Anise), 7th group treated with (STZ + Anise + BPA) and 8th group received Anise and BPA. All control and treatment were subsisted one month ago. Norepinephrine (NE), Dopamine DA and Serotonin (5HT) were determined in homogenate brain tissue by HPLC.
Results: The results showed the significant increase in NE, DA and 5HT in diabetic rats treated with (STZ), and in diabetic rats with BPA (STZ + BPA) in comparison with vehicle and diabetic (STZ) group. The results also showed a significant increase in glucose, triglyceride, cholesterol, ALT and AST. After treatment with (Anis), a significant decrease in NE, DOP and 5HT in diabetic rats treated with Anise (STZ + Anise) and diabetic rats treated with (Anise + BPA) in comparison with vehicle and diabetic (STZ) group. Thus, from this study,
Conclusion: It is concluded that, (BPA) has neurotoxic and this neurotoxicity was increased with diabetes and decreased by the treatment with anise.
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