IN VITRO AND IN VIVO SCREENING OF ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC AND AQUEOUS EXTRACTS OF ANOGEISSUS LATIFOLIA LEAVES

SABA SHAIKH; BADRUDDEEN; MOHAMMAD IRFAN KHAN; AJAZ AHMED

doi:10.22159/ijpps.2022v14i11.45593

Authors

SABA SHAIKH Department of Pharmacology, Anjuman-I-Islam Kalsekar Technical Campus, New Panvel, Navi Mumbai 410206, Maharashtra, India https://orcid.org/0000-0003-0432-1285
BADRUDDEEN Herbal Bioactive Research Laboratory, Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh, India
MOHAMMAD IRFAN KHAN Herbal Bioactive Research Laboratory, Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh, India
AJAZ AHMED Department of Pharmacognosy, Anjuman-I-Islam Kalsekar Technical Campus, New Panvel, Navi Mumbai 410206, Maharashtra, India

DOI:

https://doi.org/10.22159/ijpps.2022v14i11.45593

Keywords:

Anogeissus Latifolia, In vitro antioxidant activity, DPPH, In vitro anti-inflammatory activity, Carrageenan rat paw edema

Abstract

Objective: To evaluate and compare anti-oxidant and anti-inflammatory activity of methanolic and aqueous extract of Anogeissus latifolia leaves

Methods: The in vitro antioxidant activity was investigated using nitric oxide radical inhibition activity assay, hydroxyl radical scavenging activity assay, DPPH free radical scavenging assay, and reducing power assay. The in vitro anti-inflammatory activity was investigated using erythrocyte membrane stabilization, inhibition of protein denaturation, and proteinase inhibitory activity the in vivo anti-inflammatory activity was investigated using carrageenan-induced rat paw edema. The biochemical parameters were evaluated in the blood, which included the determination of serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase values and in the liver, which includes the estimation of lipid peroxidation, reduced glutathione, and superoxide dismutase.

Results: The methanolic extract caused a significant dose depended on the reduction of inflammation when compared with the aqueous extract of Anogeissus latifolia. The anti-inflammatory activity of all groups was found to be comparable to the standard indomethacin group. The maximum percent inhibition in paw edema was found in methanolic extract of Anogeisuss latifolia at a dose of 500 mg/kg was 53.33%, with significant anti-inflammatory activity p<0.001.

Conclusion: The leaf extract of Anogeissus latifolia possesses anti-oxidant and anti-inflammatory activity. The therapeutic effect of Anogeisuss latifolia extracts will encourage its use in the treatment of inflammation.

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