GENETIC DIVERSITY ASSESSED THROUGH RAPD MARKERS IN SYZYGIUM ALTERNIFOLIUM (WT) WALP
Keywords:
Syzigium alternifolium, Endemic, Endangered, Genetic diversity, RAPD markers and PolymorphismAbstract
Objective: The present study has been carried out to evaluate the genetic diversity in a representative population of Syzigium alternifolium from six different accessions of chittoor district.
Methods: Genomic DNA isolation, Polymerase Chain Reaction (PCR) amplication by using RAPD (OPJ, OPM series) primers, Agarose gel Electrophoresis, Jaccard's similarity coefficients, and UPGMA Dendrogram.
Results: Initially, 20 decamer primers were screened, a total of 28 bands were obtained from seven reproducible primers, with a mean of four amplified bands per primer. The primers OPJ6 and OPM2 resulted 100 % polymorphism. The plant species showed an average of 70.99% polymorphism. Cluster analysis (UPGMA) was used to generate a dendrogram based on Jaccard's similarity coefficients. A maximum similarity value of 76.5% was observed two accessions of 3 and 2 followed by 2 and 1 i.e. 71.4%. Whereas 27.8% similarity value was observed between accessions 5 and 3 and, which were found to be genetically most diverse.
Conclusion: The results suggest that, S. alternifolium possess the high level of genetic diversity, as compared to other endangered plant species. Present study concludes, RAPD is a potential molecular marker to evaluate the genetic diversity of S. alternifolium species.
Â
Downloads
References
Mohan BA, Lakshmi BB. Brief note on Syzygium alternifolium (Wight) Walp, an endemic plant species found in sri venkateswara wildlife sanctuary of andhra Pradesh, with special reference to its fruiting. Zoos’ Print J 2000;15(2):210.
Sudhakar A, Ramesh C, Nagaraju N, Vedavathy S, Sri Rama Murthy K. Pharmacognostical studies on stem & fruit of syzygium alternifolium (Wight) Walp., An Endemic to South Eastern Ghats, India. Asian J Biochem Pharm Res 2012;1(2):127-38.
Reddy CS, Reddy KN, Pattanaik C, Raju VS. Ethnobotanical observations on some endemic plants of Eastern Ghats, India. Ethnobotanical Leafl 2006;10:82–91.
Nagaraju, Rao KN. A survey of plant crude drugs of Rayalaseema, Andhra Pradesh, India. J Ethnopharmacol 1990;29:137–58.
Rao BK, Rao CH. Hypoglycemic and antihyperglycemic activity of Syzygium alternifolium (Wt.) Walp. Seed extracts in normal and diabetic rats. PhD Thesis, Sri Venkateswara University, Tirupati; 2001. p. 88-93.
Bakshu LM. Ethnomedicobotanical and phytochemical evaluation of certain rare, endemic and endangered medicinal plants from Eastern Ghats, Andhra Pradesh, India. PhD Thesis. SK. University, Anantapur; 2002.
Appala raju VVSS, Ramesh M, Lakshmi narsau M, Muralikrishna kumar M. Antimicrobial activity of the plant Syzygium alternifolium. Asian J Chem 2007;19:4923-4.
Solomon Raju AJ, Radha Krishna J, Hareesh Chandra P. Reproductive ecology of Syzygium alternifolium (Myrtaceae), an endemic and endangered tropical tree species in the southern Eastern Ghats of India. J Threatened Taxa 2014;26(9):6153-71.
Nebauer SG, del Castillo-Agudo L, Segura J. RAPD variation within and among natural populations of outcrossing willow-leaved foxglove (Digitalis obscura L.). Theor Appl Genet 1999;98:985-94.
Nebauer SG, del Castillo-Agudo L, Segura J. An assessment of genetic relationships within the genus Digitalis based on PCR-generated RAPD markers. Theor Appl Genet 2000;100:1209-16.
Mulligan GA, Findlay JN. Sexual reproduction and agamospermy in the genus Draba. Can J Bot 1970;48:269-70.
Nybon N, Bartish I. Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspect Plant Ecol, Evol Syst 2000;3:93-114.
Sanjay Lal, Kinnari N Mistry, Parth B Vaidya, Smit D Shah, Riddhi A. Thaker genetic diversity among five economically important species of asparagus collected from central gujarat (India) Utilizing RAPD Markers (Random Amplification of Polymorphic DNA). Int J Adv Biotechnol Res 2011;2(4):414-21.
Rodica Catana, Monica Mitoi, Roxana Ion. The RAPD techniques used to assess the genetic diversity in Draba dorneri, a critically endangered plant species. Adv Biosci Biotechnol 2013;4:164-9.
Ranjan Kumar Shaw, Laxmikanta Acharya, Arup Kumar Mukherjee. Assessment of genetic diversity in a highly valuable medicinal plant Catharanthus roseus using molecular markers. Crop Breed Appl Biotechnol 2009;9:52-9.
Sebastian VA, D Cruz L, Braganza VJ. Assessment of genetic diversity within and among populations of Tylophora rotundifolia using RAPD markers. Gene Conserve 2010;36:94-117.
Rossetto M, Weaver PK, Dixon KW. Use of RAPD analysis in devising conservation strategies for the rare and endangered Grevillea scapigera (Proteaceae). Mol Ecol 1995;4:321-9.
Murray HG, Thompson WF. Rapid isolation of high molecular weight DNA. Nucleic Acids 1980;8:4321-5.
Xiao LQ, Ge XJ, Gong X, Hao G, Zheng SX. ISSR variation in the en endemic and endangered plant Cycas guizhouensis (Cycadaceae). Ann Bot 2004;94:133–8.
Jaccard P Nouvelles Recherches sur la distribution florale. Bull Soc Vaudoise Sci Nat 1908;44;223-70.
Li FG, Xia NH. Population structure and genetic diversity of an endangered species, Glyptostrobus pensilis (Cupressaceae). Bot Bull Acad Sin 2005;46:155–62.
Ge YQ, Qiu YX, Ding BY, Fu CX. A ISSR analysis on population genetic diversity of the relict plant Ginkgo biloba. Biodiversity Sci 2003;11:276-87.
Brauner SD, Crawford J, Stuessy TF. Ribosomal DNA and RAPD variation in the rare plant family Lactoridaceae. Am J Bot 1992;79:136-43.
Wang XQ, Zou YP, Zhang DM, Hong DY. RAPD analysis for genetic polymorphism in Cathaya argyrophylla. Sci Chin 1996;26:437-41.
Pei YL, Zou YP, Yin Z, Wang XQ, Zhang ZX, Hong DY. Preliminary report of RAPD analysis in Paeonia suffruticosa subsp. spontanea and P. rockii. Acta Phytotaxon Sin 1995;33:350-6.
Su YJ, Wnag T, Huang C, Zhu JM, Zhou Q. RAPD analysis of different population of Dacydium pierrei. Acta Sci Natl University of Sunyatseni 1999;38:99-101.